Agilent 5100 synchronous vertical dual view icp oes

Prior to metal determination, brain tissues were digested according to the analytical method of APHA (2017 ). Brain contents of iron (Fe) and platinum (Pt) were quantified in brain tissues of different groups. After excision and dissection, brain samples were digested using a 1:3 volumetric mix of hydrochloric acid to nitric acid. Digestions were incubated at 70 °C for up to 12 h until all tissues had gone into solution and a clear liquid is obtained. Determination of Fe and Pt contents was quantified using an inductively coupled plasma optical emission spectrometer (ICP-OES) (Agilent 5100 Synchronous Vertical Dual View (SVDV) ICP-OES, with Agilent Vapor Generation Accessory VGA 77, Agilent Technologies). For each series of measurements, intensity calibration curve was constructed composed of a blank and three or more standards from Merck Company (Germany). Accuracy and precision of the metal measurements were confirmed using external reference standards from Merck, and standard reference material for trace elements in water and quality control sample from the National Institute of Standards and Technology (NIST) were used to confirm the instrument reading. The detection limit of the instrument was 0.01 ppb.