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Rabbit anti acetyl α tubulin

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Rabbit anti-acetyl-α-tubulin is a primary antibody that specifically recognizes acetylated α-tubulin, a post-translational modification of the α-tubulin subunit of microtubules.

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Lab products found in correlation

Mouse anti α tubulin, by Merck Group (3 mentions) Alexa fluor dye, by Thermo Fisher Scientific (3 mentions) Protein assay system, by Bio-Rad (2 mentions) Rabbit anti gapdh, by Cell Signaling Technology (2 mentions) Rabbit anti macf1, by Santa Cruz Biotechnology (2 mentions) Rabbit anti cux1, by Santa Cruz Biotechnology (2 mentions) Chicken anti gfp, by Thermo Fisher Scientific (2 mentions) Rabbit anti gfp, by Thermo Fisher Scientific (2 mentions) Ecl reagent, by Cytiva (1 mentions) Chicken anti nestin, by Neuromics (1 mentions) Rabbit anti tbr1, by Merck Group (1 mentions) Goat anti brn1, by Novus Biologicals (1 mentions) Mouse anti map2, by Fortrea (1 mentions) Ecl reagent, by Cell Signaling Technology (1 mentions) Mouse anti gsk3β, by BD (1 mentions) Alexa568 phalloidin, by Thermo Fisher Scientific (1 mentions) Rabbit anti phospho histone h3, by Cell Signaling Technology (1 mentions) Mab337, by Merck Group (1 mentions) Rabbit anti ki67, by Cell Signaling Technology (1 mentions) Mouse anti brdu, by BD (1 mentions)

5 protocols using rabbit anti acetyl α tubulin

1

Acetyl-α-Tubulin Expression Analysis

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Western blotting was performed as described previously [68 (link),69 (link)]. Lysates from E14.5 telencephalon were prepared using RIPA buffer and the protein content was determined by a Bio-Rad Protein Assay system. Proteins were separated on 4–12% SDS-PAGE gradient gel and transferred onto nitrocellulose membrane. Then the membrane was incubated with rabbit anti-acetyl-α-tubulin (Cell Signaling), mouse anti-α-tubulin (Sigma), or rabbit anti-GAPDH (Cell Signaling) at 4°C overnight. Appropriate secondary antibodies conjugated to HRP were used (Cell Signaling) and the ECL reagents (Amersham) were used for immunodetection. For quantification of band intensity, blots from 3 independent experiments for each molecule of interest were used. Signals were measured using ImageJ software and represented by relative intensity versus control. GAPDH was used as an internal control to normalize band intensity.
Ka M, & Kim W.Y. (2015). Microtubule-Actin Crosslinking Factor 1 is required for dendritic arborization and axon outgrowth in the developing brain. Molecular neurobiology, 53(9), 6018-6032.
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2

Immunolabeling of Embryonic Neural Cells

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Immunohistochemical labeling of embryonic brain sections or dissociated neural cells was performed as described previously (Kim et al., 2009 ). The following primary antibodies were used: rabbit anti-MACF1 (Wu et al., 2011 (link)), rabbit anti-MACF1 (Santa Cruz), rabbit anti-phospho-MACF1 (Wu et al., 2011 (link)), chicken anti-nestin (Neuromics), mouse anti-MAP2 (Covance), rabbit anti-Tbr1 (Chemicon), rabbit anti-Cux1 (Santa Cruz), goat anti-Brn1 (Novus Biologicals), rabbit anti-acetyl-α-tubulin (Cell Signaling), mouse anti-α-tubulin (Sigma), and chicken anti-actin (Millipore). Appropriate secondary antibodies conjugated with Alexa Fluor dyes (Invitrogen) were used to detect primary antibodies.
Ka M., Jung E.M., Mueller U, & Kim W.Y. (2014). MACF1 regulates the migration of pyramidal neurons via microtubule dynamics and GSK-3 signaling. Developmental biology, 395(1), 4-18.
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3

Telencephalon Protein Analysis by Western Blot

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Lysates from E14.5 telencephalon were prepared using RIPA buffer and the protein content was determined by a Bio-Rad Protein Assay system. Proteins were separated on 3%-8% or 4%-12% SDS-PAGE gradient gel and transferred onto nitrocellulose membrane. Then the membrane was incubated with rabbit anti-MACF1 (Wu et al., 2011 (link)), rabbit anti-MACF1 (Santa Cruz), rabbit anti-phospho-MACF1 (Wu et al., 2011 (link)), mouse anti-GSK-3β (BD Biosciences), rabbit anti-acetyl-α-tubulin (Cell Signaling), mouse anti-α-tubulin (Sigma), or rabbit anti-GAPDH (Cell Signaling) at 4 °C overnight. Appropriate secondary antibodies conjugated to HRP were used (Cell Signaling) and the ECL reagents (Amersham) were used for immunodetection.
For quantification of band intensity, blots from 3 independent experiments for each molecule of interest were used. Signals were measured using ImageJ software and represented by relative intensity versus control. GAPDH was used as an internal control to normalize band intensity.
Ka M., Jung E.M., Mueller U, & Kim W.Y. (2014). MACF1 regulates the migration of pyramidal neurons via microtubule dynamics and GSK-3 signaling. Developmental biology, 395(1), 4-18.
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4

Immunohistochemical Labeling of Neural Cells

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Immunohistochemical labeling of embryonic brain sections or dissociated neural cells was performed as described previously [65 (link),66 (link)]. The following primary antibodies were used: Chicken anti-GFP (Invitrogen), Rabbit anti-GFP (Invitrogen), mouse anti-β-III Tubulin (Phosphosolutions), rabbit anti-acetyl-α-tubulin (Cell Signaling), and mouse anti-β-tubulin (Upstate). Appropriate secondary antibodies conjugated with Alexa Fluor dyes (Invitrogen) were used to detect primary antibodies. Polymerized F-actins were detected by labeling with phalloidin-Alexa 568 (Invitrogen).
Ka M, & Kim W.Y. (2015). Microtubule-Actin Crosslinking Factor 1 is required for dendritic arborization and axon outgrowth in the developing brain. Molecular neurobiology, 53(9), 6018-6032.
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5

Immunostaining of Brain Cells

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Immunostaining of brain sections or dissociated neural cells was performed as described previously (Kim et al. 2004 (link); Ka et al. 2016b (link)). The following primary antibodies were used: Mouse anti-NeuN (EMD Millipore, MAB337), rat anti-neural cell adhesion molecule L1 (EMD Millipore, MAB5272), rabbit anti-TBR1 (Abcam, ab31940), rabbit anti-CUX1 (Santa Cruz, sc-13024), chicken anti-MAP2 (Abcam, ab5392), rabbit anti-GFAP (Abcam, ab7260), rabbit anti-Ki67 (Cell Signaling, #9129), rabbit anti-phospho-Histone H3 (Cell Signaling, #9701S), mouse anti-BrdU (BD Biosciences, 555627), chicken anti-Tbr2 (EMD Millipore, ab15894), rabbit anti-BLBP (Abcam, ab32423), mouse anti-Nestin (PhosphoSolutions, 1435-NES), rabbit anti-ARL13B (Abcam, ab83879), rabbit anti-Acetyl-α-Tubulin (Cell Signaling, #5335), chicken anti-GFP (Thermo Fisher, A10262), rabbit anti-GFP (Thermo Fisher, A11122) and mouse anti-MACF1 (Santa Cruz, sc-377532). Alexa Fluor568-Phalloidin (Thermo Fisher, A12380) was used to stain polymerized actin. Appropriate secondary antibodies conjugated with Alexa Fluor dyes (Thermo Fisher) were used to detect primary antibodies. The purpose of using each antibody is listed in Table1.
Ka M., Moffat J.J, & Kim W.Y. (2021). MACF1 involved in the 1p34.2p34.3 microdeletion syndrome is essential in cortical progenitor polarity and brain integrity. Cellular and molecular neurobiology, 42(7), 2187-2204.
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