Blood was collected at 4 weeks after treatment with sivelestat sodium hydrate while rats were under anesthesia (35 mg/kg pentobarbital) and centrifuged at 12,000 × g for 10 min at 4°C. The supernatant was collected and used to determine the levels of TNF-α (EM010-96) and IL-6 (EM004-96) production and using commercial enzyme-linked immunosorbent assay (ELISA; ExCell Bio, Taichang, China) kits and an ELISA reader (Bio-Rad Laboratories, Inc.) at 405 nm. Nitrite concentrations were measured using a commercial kit (A038; Nanjing Jiancheng Biology Engineering Institute, Nanjing, China) and an ELISA reader (Bio-Rad Laboratories, Inc.) at 540 nm. HMGB1 secretion were measured using a commercial kit (E-EL-R0505c; Elabscience Biotechnology Co., Ltd., Wuhan, China) at 450 nM.
Elisa reader
Manufactured by Bio-Rad
Sourced in United States, Germany, Japan, China, Canada, United Kingdom, Singapore, Switzerland
The ELISA reader is a laboratory instrument designed to measure the optical density of samples in enzyme-linked immunosorbent assay (ELISA) experiments. It is used to quantify the amount of a specific substance, such as a protein or antibody, present in a sample by detecting the intensity of a color change or luminescent signal.
Automatically generated - may contain errors
Lab products found in correlation
Methyl thiazolyl tetrazolium (mtt), by Merck Group (30 mentions)
Dimethyl sulfoxide (dmso), by Merck Group (23 mentions)
Mtt reagent, by Merck Group (8 mentions)
Xtt assay kit, by Roche (7 mentions)
Mtt solution, by Merck Group (6 mentions)
Bovine serum albumin (bsa), by Merck Group (4 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions)
Cell counting kit 8 cck 8, by Dojindo Laboratories (3 mentions)
Tmb solution, by Thermo Fisher Scientific (3 mentions)
96 well plate, by Corning (3 mentions)
Dulbecco modified eagle medium (dmem), by Euroclone (3 mentions)
Cck 8, by Dojindo Laboratories (2 mentions)
96 well elisa plate, by Corning (2 mentions)
Dimethyl sulfoxide (dmso), by Thermo Fisher Scientific (2 mentions)
Dimethyl sulfoxide solution, by Merck Group (2 mentions)
Mtt solution, by Boehringer Ingelheim (2 mentions)
Methyl thiazolyl tetrazolium (mtt), by Promega (2 mentions)
Methyl thiazolyl tetrazolium (mtt), by Bio-Rad (2 mentions)
Fibronectin, by Merck Group (2 mentions)
Cellquest software, by BD (2 mentions)
446 protocols using elisa reader
1
Assessing Inflammatory Markers in Rat Model
2
Optimizing Nisin Preconditioning of MSCs
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Three concentrations (100, 200, and 300 IU/mL) of Nisin were evaluated for the optimal Nisin dosage used for the preconditioning of MSCs on the 1st, 3rd, and 5th days of culture. In each well of a 48-well plate 1 × 104, MSCs were cultured and incubated at 37 °C and 5% CO2. After the 1st, 3rd, and 5th days of culture, 200 μl of MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) solution (5 mg/mL) added to each well, and the plate incubated for 3 h. Then, 100 μl of Dimethyl sulfoxide (DMSO) solution was added, and an ELISA reader (Biorad, USA) read the absorption at 540–630 nm by an ELISA reader (Biorad, USA). The experiment was performed three times for each dose [16 (link)].
3
VSMC Proliferation Analysis via BrdU and MTT
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VSMC proliferation was analyzed via measuring DNA synthesis with a colorimetric bromodeoxyuridine (BrdU) ELISA kit according to the manufacturer's instructions (Shanghai JinMa Laboratory and Equipment Company, Shanghai, China). Briefly, VSMCs were seeded in 24-well plates at a density of 5 × 10 4 cells/well. Three days after gene transfer, the cells were stimulated with recombinant mouse TNF-α (10 ng/ml) for 24 or 48 h, after which the cell culture supernatants were collected and centrifuged at 12 000 g for 15 min at 4 °C; the extracted supernatants were immediately frozen at -80 °C until use. Absorbance values at 450 nm were determined using an ELISA reader (Bio-Rad, CA, USA).
In a separate set of experiments, VSMC proliferation was evaluated using the methyl thiazolyl tetrazolium (MTT) assay. Briefly, VSMCs were plated in 96 multi-well plates at a density of 5 × 10 3 cells/well. Three days after gene transfer, the cells were stimulated with recombinant mouse TNF-α (10 ng/ml) for 24 or 48 h, and then 0.5 mg/ml of MTT in PBS was added to each well. After 4 h, the cell culture supernatants were removed and 150 μl of DMSO was added to each well to dissolve the crystals. The absorbance values at 490 nm were obtained using an ELISA reader (Bio-Rad).
In a separate set of experiments, VSMC proliferation was evaluated using the methyl thiazolyl tetrazolium (MTT) assay. Briefly, VSMCs were plated in 96 multi-well plates at a density of 5 × 10 3 cells/well. Three days after gene transfer, the cells were stimulated with recombinant mouse TNF-α (10 ng/ml) for 24 or 48 h, and then 0.5 mg/ml of MTT in PBS was added to each well. After 4 h, the cell culture supernatants were removed and 150 μl of DMSO was added to each well to dissolve the crystals. The absorbance values at 490 nm were obtained using an ELISA reader (Bio-Rad).
4
Cytotoxicity Evaluation of Adriamycin
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Effects of a chemotherapy agent on the high clone and low clone cells were evaluated using CCK‐8 (Dojindo) according to the manufacturer’s protocol. The clone cells were plated at 15,000 cells/well in a 96‐well plate with 100 μL culture medium. After 24 hours, the culture medium was replaced and Adriamycin was directly added at various concentrations. After 24‐h treatment, the cells were incubated with 10 μL CCK‐8 reagent for 4 hours at 37°C in a 5% CO2 incubator. Absorbance at 450 nm was measured with a Bio‐Rad ELISA reader (Bio‐Rad).
5
Quantification of Cytokine and Chemokine Levels
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The supernatants of the lung homogenates were assayed for the determination of cytokines and chemokine. IL-33 and CCL17 were measured via the package of DuoSet ELISA (R&D Systems) and IL-13, IL-5, and total IgE were measured with a Ready-SET-Go! ® ELISA (eBioscienceTM (IL13, total IgE) and InvitrogenTM IL-5, USA) kit. All ELISA assays were performed according to the manufacturer's protocol. The concentrations of the measured cytokines and chemokines were expressed as pg/mg protein in lung homogenates and pg/mL in serum. The ELISA plates were read at 450 nm using a Bio-Rad ELISA Reader (Bio-Rad, Hercules, CA, USA) (3 (link), 4 (link)).
6
Cytotoxicity of HVMEE in HT-29 and SW620 Cells
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HT-29 and SW620 cells were seeded in 96-well plates at a density of 2×104 cells/well for 24 h, then cells were treated with 0.01, 0.03, 0.1, 0.3, 1, and 3 mg/ml HVMEE for 24 h in complete medium. Following treatment, 20 µl of MTT solution (5 mg/ml) was added to each well for 4 h. The cells were then washed three times with PBS, and the resulting formazan was resuspended in 150 µl dimethyl sulfoxide. Absorbance was measured at 570 nm with a Bio-Rad ELISA reader (Bio-Rad Laboratories, Inc., Hercules, CA, USA). The experiment was repeated independently three times.
7
Temporal Serum Biomarker Profiling
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Blood samples were collected at 4 time points: pre-bypass, post-bypass, 24, and 48 hours after surgery. Serum was stored at À80 C and the final biochemical analysis was made using ELISA with the Bio-Rad ELISA reader (Bio-Rad Laboratories Inc, Hercules, Calif).
8
Cell Viability Assay of SW480 Cells
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SW480 cells were plated in 96-well plates at a density of 2,000 cells/well. The cell viability was assessed on d 1, 2, 3, 4 and 5. Cell counting Kit-8 (CCK8) (10 µL, Dojindo Laboratories, Japan) was added into each well. After 4 h of incubation, the optical density of each well was measured at 450 nm with an enzyme linked immunosorbent assay (ELISA) reader (Bio-Rad).
9
Antioxidant Enzyme Activity in Mice
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At the end of the experiment (14 days), all mice were anesthetized with dimethyl ether and sacrificed by neck dissection. Blood and liver tissue samples were collected. Liver samples were incubated in 0.1 m phosphate buffer (10% w/v) [26 (link)] and then centrifuged with the supernatant being frozen at −70°C.The Bio-Rad ELISA reader was used following the manufacturer’s instructions to determine MDA [27 (link)], SOD [28 (link)] and CAT [29 (link)] activity levels in liver homogenate. The experiment was modeled after similar previous studies.
10
Klf4 Modulates Progesterone Secretion
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GCs (1 × 105 cells/well) were transfected with Klf4 expression plasmid (0.1 and 0.3 μg/well) or Klf4 siRNA (200 nM/well). After 6 h, they were transferred to LH (200 ng/mL) or control medium for 24–36 h, and conditioned media were harvested for the hormone assay. Progesterone levels were measured using an enzyme-linked immunosorbent assay (ELISA) kit (CSB-E07282r, Cusabio Biotech Co., Ltd., Wuhan, China) following the manufacturer’s protocol. Intra- and inter-assay coefficients of variation were less than 15%, and the limit of detection was 0.8 ng/mL under our conditions. To achieve concentrations within the assay range, the conditioned media were diluted, and serial dilutions were found to behave in a linear fashion using progesterone standards. Absorbance was read against a blanking well at 450 nm within 15 min in an ELISA Reader (BioRad, Hercules, CA, USA). All samples were run in duplicate. Data were collected from three independent experiments.
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