3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), bafilomycin A1 (Baf), wortmannin, monodansylcadaverine (MDC), 3-(4,5-dimethyl thiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT), triphenyltetrazolium chloride (TTC) were purchased from Sigma. Antibodies against beclin-1 (3495), LC3B (#2775), p62(5114S) and Bax were obtained from Cell Signaling Technology, Danvers, Massachusetts, USA, anti-Bcl-2(SC-7382) was from Santa Cruz Biotechnology, USA. β-actin (TA-09) was from ZSGB-BIO, Beijing, China. Antimyoactin antibody, was obtained from Wuhan Boster Biological Technology, LTD., China. Lipofectamine RNAiMAX kit was purchased from Invitrogen Company.
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide
Manufactured by Merck Group
Sourced in United States, Germany, India, China, Italy, Spain, Sao Tome and Principe, Mexico, United Kingdom, Macao, Canada, France, Australia, Portugal
3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide is a lab equipment product. It is a colorimetric assay used for measuring cell metabolic activity.
Automatically generated - may contain errors
Lab products found in correlation
Dimethyl sulfoxide (dmso), by Merck Group (107 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (67 mentions)
Propidium iodide, by Merck Group (33 mentions)
Bovine serum albumin (bsa), by Merck Group (28 mentions)
Triton x 100, by Merck Group (24 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (23 mentions)
Quercetin, by Merck Group (22 mentions)
N hydroxysuccinimide, by Merck Group (19 mentions)
Streptomycin, by Thermo Fisher Scientific (19 mentions)
2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (19 mentions)
Gallic acid, by Merck Group (18 mentions)
Fetal bovine serum (fbs), by Merck Group (17 mentions)
Penicillin, by Thermo Fisher Scientific (16 mentions)
Rpmi 1640, by Thermo Fisher Scientific (16 mentions)
Trypsin edta, by Thermo Fisher Scientific (16 mentions)
Phosphate buffered saline (pbs), by Merck Group (14 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (14 mentions)
Streptomycin, by Merck Group (14 mentions)
Acridine orange, by Merck Group (12 mentions)
β actin, by Cell Signaling Technology (12 mentions)
429 protocols using 3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide
1
Deciphering Autophagy Pathways: A Comprehensive Protocol
2
Functionalization of MoS2 Nanosheets
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We purchased monolayer MoS2 nanosheets (62 μg mL-1, dissolved in ethanol) from 2D-Semiconductors. mPEG-NH2 (5K) and NH2-PEG-Boc (5K) from Laysan Bio, Inc (USA). We purchased Lipoic acid (LA), Folic acid (FA), trifluoroacetic acid (TFA), dichloromethane, N,N'-dicyclohexylcarbodiimide (DCC), triethylamine (TEA), sodium bicarbonate, N-Hydroxysuccinimide (NHS), dimethyl sulfoxide (DMSO), diethyl ether, Poly(allylamine hydrochloride) (PAH, molecular weight 15,000), and 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromides (MTT) from Sigma Aldrich. Antibodies against HDAC1 (ab53091), KRAS (ab55391), and Actin (ab8227) were obtained from Abcam. We used ultrapure DI water for conducting all experiments (Milli-Q Integral 5).
3
Antioxidant Capacity Evaluation Protocol
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Bicinchoninic acid (BCA) solution, bovine serum albumin (BSA), t-butyl hydroperoxide (t-BHP), butylated hydroxytoluene (BHT), CCl4, 1,1-diphenyl-2-picrylhydrazyl (DPPH), formalin, sodium carboxymethylcellulose (CMC), 2′,7′-dichlorofluorescin diacetate (DCFDA), phosphoric acid, trichloroacetic acid (TCA), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromides (MTT), and Trolox were obtained from Sigma-Aldrich Co. 2-Thiobarbituric acid (TBA) was obtained from Tokyo Chemical Industry Co. Malondialdehyde (MDA) tetrabutylammonium salt was obtained from Fluka. Oligonol is commercially available (Amino Up Chemical Co., Ltd., Sapporo, Japan).
4
Synthesis and Characterization of Hybrid Nanoparticles
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Hexadecyltrimethylammonium bromide (CTAB, > 98.0%), L-Ascorbic acid (BioUltra, ≥99.5%), silver nitrate (AgNO3, >99%), sodium borohydride (NaBH4, 99%), Poly (3,4-ethylenedioxythiophene)/poly (styrenesulfate) (PEDT/PSS, molecular weight 240,000), Poly (allylamine hydrochloride) (PAH, molecular weight 15,000) doxorubicin hydrochloride (DOX, 98%) and 3-(4,5-dimethylthiazol-2-yl)-2,5 -diphenyltetrazolium bromides (MTT) were purchased from Sigma Aldrich. Gold (III) chloride trihydrate (HAuCl4· 3H2O) was purchased from Acros Organics. Sodium salicylate (99%) was purchased from Alfa Aesar. Ultrapure water produced with a Milli-Q Integral 5 system was used in all experiments.
5
Isolation and Identification of FC from Ferula assafoetida
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FC (Figure 1 A) was isolated and identified from Ferula assafoetida as previously described [12 (link)]. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), Bcl-2, and β-actin were purchased from Sigma-Aldrich (Sigma-Aldrich, St. Louis, MO, USA). Also, specific antibodies for Cyclin D1, Cyclin E, Cyclin B1 were bought from Santa Cruz Biotechnology (Santa Cruz Biotechnology, Dallas, TX, USA). PARP, HDAC1, and HDAC2 were purchased from Cell Signaling (Cell signaling Technology, Danvers, MA, USA) for Western blot analysis.
6
Ginseng Impact on Fibroblast Viability
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HDFs were purchased from the Korean Cell Line Bank (Seoul, South Korea). The fibroblasts were grown in Dulbecco's modified Eagle's medium supplemented with 10% fetal bovine serum, 2 mM glutamine, and 1% penicillin–streptomycin–amphotericin B (10,000 U/mL, 10 g/mL, and 25 mg/mL, respectively; Gibco, Waltham, MA, USA) at 37 °C in a humidified incubator containing 5% CO2. Cell viability was analyzed as a function of ginseng concentration and treatment time using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (Sigma-Aldrich, St. Louis, MO, USA) assay. The cells were plated into wells at a density of 2 × 104 cells/well. KRG was added to the wells at various concentrations (0, 0.2, 0.4, 0.8, 1.0, and 2.0 mg/mL) and incubated for increasing durations (0, 6, 12, and 24 h). After incubation, EZ-Cytox solution (DoGen Bio, Seoul, Kores) was added to each well at 10 μL/well, and the plates were incubated at 37 °C for 1 h. The absorbance was measured at 450 nm using a Multiskan EX ELISA microplate reader (Thermo Fisher Scientific, Rockford, IL, USA).
7
Cytotoxicity and Apoptosis Evaluation
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FD (NIES CRM No. 28) was obtained from the National Institute for Environmental Studies (Ibaraki, Japan). Dulbecco’s Modified Eagle Medium (DMEM), Ham’s F-12 nutrient mix, Fetal bovine serum (FBS), and penicillin-streptomycin mixture were purchased from GIBCO INC., NY, USA. Ethidium bromide, acridine orange, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), 2′ 7′-dichlorodihydrofluorescein diacetate (DCF-DA), Triton™ X-100, Hoechst 33342, and paraformaldehyde were obtained from Sigma-Aldrich Co (St. Louis, MO, USA). SuperSignal™ West Pico PLUS Chemiluminescent Substrate was purchased from Thermo Fisher Scientific (Rockford, IL, USA). Ace-α-® cDNA synthesis kit was purchased from ReverTra (Toyobo, Osaka, Japan). PCR primers were purchased from Bioneer Inc. (Daejeon, South Korea). Primary and secondary antibodies for western blot analysis, goat serum, Prolong® Gold AntiFade Reagent with DAPI, and DyLight™ 554 Phalloidin were bought from Cell Signaling Technology (Beverly, MA, USA).
8
Antioxidant and Enzyme Assays
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Butylated hydroxyanisole (BHA, ≥98.5%), chlorogenic acid (European Pharmacopoeia Reference Standard), diclofenac (≥98%), kojic acid (≥98.5%), (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide, collagenase from Clostridium histolyticum, hyaluronidase from bovine testes, mushroom tyrosinase and porcine pancreas elastase were purchased from Sigma-Aldrich (St. Louis, MO, USA). Soybean LOX was a product from TCI chemicals (Tokyo, Japan). Acetonitrile was HPLC grade. Other reagents and chemicals were of analytical grade.
9
Synthesis and Characterization of NQC Compound
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N′-(Pyridine-3-carbonyl)quinoxaline-2-carbohydrazide (NQC) was synthesised and purified to 99.2% purity in International Medical University (IMU). Structure of the quinoxaline-2-carbohydrazide derivative was confirmed by spectroscopic methods. All solvents used in this research were of high performance column chromatography (HPLC) grade. Chemical reagents including formic acid and dipotassium phosphate (K2HPO4) from Fisher Scientific, dimethyl sulfoxide (DMSO) and monopotassium phosphate (KH2PO4) from Merck, and acetonitrile (ACN) from Friedemann Schmidt were used. (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide), sulphanilamide, N-(1-naphthyl)ethylenediamine and biological grade DMSO were procured from Sigma-Aldrich Sdn. Bhd, Malaysia. β-NADPH was procured from Sigma Aldrich, USA. RAW 264.7 cells and LPS (Escherichia coli O111:b4) were procured from American Type Culture Collection, Manassas, USA. HLM, RLM and MLM (20 mg/mL, Catalog #HMMCPL, Gibco) was procured from Life Technologies, Singapore.
Synthesis of N′-(pyridine-3-carbonyl)quinoxaline-2-carbohydrazide is as shown in Fig.4 .![]()
Synthesis of N′-(pyridine-3-carbonyl)quinoxaline-2-carbohydrazide is as shown in Fig.
Synthetic route of N′-(pyridine-3-carbonyl) quinoxaline-2-carbohydrazide (NQC)
10
Melanogenesis Inhibition Protocol
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Dulbecco’s Modified Eagle’s Medium, fetal bovine serum and penicillin/streptomycin (100 IU/50 μg/mL) were obtained from Invitrogen (Grand Island, USA). Antibodies against tyrosinase, TRP-1, TRP-2 and β-actin were purchased from Abcam (Cambridge, USA). All other chemicals including 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide, α-MSH, dimethyl sulfoxide (DMSO), mushroom tyrosinase and L-DOPA were purchased from Sigma-Aldrich (USA).
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