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2 protocols using kif2a

1

Gene Expression Analysis of Adipose-Derived Stem Cells

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Total RNAs of ASCs were extracted with RNeasy Mini kit (QIAGEN, Hilden). Reverse transcription was performed using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Darmstadt), as instructed. All probes for gene analysis were obtained from Applied Biosystems: ADIPOQ (#Hs00605917_m1), AURKA (#Hs01582072_m1), AURKB (#Hs00945858_g1), CCP110 (#Hs00206922_m1), PLK1 (#Hs00153444_m1), PLK4 (#Hs00179514_m1), KIF2A (#Hs00189636_m1), KIF24 (#Hs00950248_m1), HDAC6 (#Hs00195869_m1), SMO (#Hs01090242_m1), GLI1 (#Hs00171790_m1), NANOG (#Hs04260366_g1), PTCH1 (#Hs00181117_m1), RUNX2 (#Hs01047973_m1), KLF4 (#Hs00358836_m1), c-MYC (#Hs00153408_m1), KLF6 (#Hs00810569_m1), PPARγ (#Hs01115513_m1), LEPTIN (#Hs00174877_m1), OCT4 (#Hs04260367_gH), SOX2 (#Hs01053049_s1), and GAPDH (#Hs02758991_g1). Real-time PCR was performed with a StepOnePlus Real-time PCR System (Applied Biosystems). The data were analyzed using StepOne Software v.2.3 (Applied Biosystems) as described previously [11 (link)].
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2

Gene expression analysis of ASCs

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Total RNAs of ASCs were extracted with RNeasy Mini kit (7Bioscience, Neuenburg, Germany). Reverse transcription was performed while using High-Capacity cDNA Reverse Transcription Kit (Promega, Mannheim, Germany), as instructed. All the probes for gene analysis were obtained from Applied Biosystems: ADIPOQ (#Hs00605917_m1), IL-6 (#Hs00985639_m1), IL-8 (#00175123_m1), IL-10 (#Hs00961622_m1), TNFα (Hs00174128_m1), PLK1 (#Hs00153444_m1), PLK4 (#Hs00179514_m1), KIF2A (#Hs00189636_m1), SMO (#Hs01090242_m1), GLI1 (#Hs00171790_m1), NANOG (#Hs04260366_g1), PTCH1 (#Hs00181117_m1), RUNX2 (#Hs01047973_m1), KLF4 (#Hs00358836_m1), c-MYC (#Hs00153408_m1), PPARγ (#Hs01115513_m1), LEPTIN (#Hs00174877_m1), SOX2 (#Hs01053049_s1), EpCAM (Hs0090188s_m1), VIM (#Hs00958111_m1), SNAIL1 (Hs00195591_m1), TWIST (Hs01675818_s1), ZEB1 (Hs01566408_m1), and GAPDH (#Hs02758991_g1). Real-time PCR was performed with a StepOnePlus Real-time PCR System (Applied Biosystems). The data were analyzed while using StepOne Software v.2.3 (Applied Biosystems), as described previously [23 (link)].
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