For the rotarod test, mice were trained three times by being placed in a neutral position in the stationary 3‐cm‐diameter cylinder of the rotarod apparatus (Singa RT‐01) with a constant rotating speed of 5 rpm for 30 s. The mice were provided 30 min breaks between the training and test phases. In the test phase, the constant rotating speed was set at 10, 15, and 20 rpm, and the time to falling was recorded when each mouse fell off the rod. For analysis of grip force, before experiments, mice were placed on the electronic balance (Denver Instrument, Bohemia, NY, USA) until they were stable to measure body weight for normalization with grip force strength. The mouse forepaws were placed on the grasping bar of the Grip‐Strength Meter (Somatco, Basile, Italy), and the mouse tail was then lifted and pulled backward until the mouse released the grasping bar. The data were collected from the peak amplifier.
Electronic balance
Manufactured by Sartorius
Sourced in Germany, China, Japan, United States
The Electronic Balance is a precision weighing instrument used to measure the mass of various objects or substances with a high degree of accuracy. It utilizes electronic sensors and digital displays to provide precise measurements, ensuring reliable and consistent results.
Automatically generated - may contain errors
Lab products found in correlation
Streptozotocin (stz), by Merck Group (2 mentions)
Optical microscope, by Nikon (1 mentions)
Stereomicroscope, by Nikon (1 mentions)
Eclipse ni u, by Nikon (1 mentions)
60 mm petri dish, by Corning (1 mentions)
Mesenchymal stem cell medium, by ScienCell (1 mentions)
Continuous wavelength multifunctional microplate reader, by Tecan (1 mentions)
Accu chek active glucometer, by Roche (1 mentions)
Whatman no 1 filter paper, by Cytiva (1 mentions)
Sl18 linear accelerator, by Philips (1 mentions)
Stereomicroscope, by Olympus (1 mentions)
Rna extraction kit, by CoWin Biotech (1 mentions)
Superscript 3 first strand kit, by Thermo Fisher Scientific (1 mentions)
Agilent 1200, by Agilent Technologies (1 mentions)
E1170, by Solarbio (1 mentions)
Ethylene diamine tetraacetic acid (edta), by Solarbio (1 mentions)
Accu chek active, by Roche (1 mentions)
Simca p 14, by Sartorius (1 mentions)
Ft nir spectrometer, by PerkinElmer (1 mentions)
Ultrapure water meter, by Merck Group (1 mentions)
70 protocols using electronic balance
1
Rotarod and Grip Strength Assessment
2
Dispersing Nanofillers in Acrylic Resin
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The ZrO 2 nanofillers (5 wt%) were added to the resin monomer, and then mixed through the extreme sonication of the fillers. The NPs were suspended in the liquid monomer and well dispersed in the liquid with the use of a sonication probe at 120 V and 60 kHz (Soniprep 150; MSE (UK) Ltd., London, UK), and then separated into individual nanocrystals for 3 min. 15 (link) To prevent the particle aggregation and segregation as much as possible, the liquid monomer of methyl methacrylate (MMA) with ZrO 2 NPs was blended with the acrylic powder instantly. All the proportions and the manipulations of the acrylic resin were in accordance with the manufacturer's instructions. The recommended mixing ratio was 10 mL of liquid monomer and 22 g of powder polymer, which represented a 3:1 volume ratio. The mixture was left aside until it reached the dough stage. According to the ISO 9001 standard, an electronic balance (the management system certified up to an accuracy of 0.0000 g; Denver Instrument, Göttingen, Germany) was used for measuring the weight of the material. For TiO 2 and Ag NPs, the same procedure was followed, whereas the usual procedure was applied for the control group -according to the manufacturer's instructions, but without any additions.
3
Pulmonary Edema Quantification Protocol
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The freshly harvested lung tissues were excised, blotted dry, and weighed using an electronic balance (Sartorius) to obtain the wet weight (W), and then dried in an oven at 60°C to obtain the dry weight (D) until the weight was not changed. To assess the degree of pulmonary edema, W/D ratio was determined by the following formula: W/D × 100%.
4
Extraction and Analysis Protocol
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Electronic balance (Sartorius), vortex oscillators, ultra-clean table (Boxum), ultra-pure water system, glass double tube, parafilm sealing film, shade cloth, Millipore disposable filter (0.45 μm), glass test tube, gauze, stereomicroscope (Nikon), dissecting forceps, grinding bead, grinding rod, optical microscope (Nikon), blood cell counting plate, HYG-full temperature shaker cabinet, THZ-D table thermostatic oscillator, autoclave, 250 mL conical flask, sterile drug sensitive tablets.
5
Comprehensive Plant Morphological Analysis
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We measured the height, stem diameter, internode length, leaf area and thickness, stomata, root system, and whole weight of sampled plants. We also determined their fresh weight using an electronic balance (Sartorius, China), and their dry weight after drying to a constant weight at 65°C. Leaf thickness was analyzed using a digital caliper (Meinaite, China). We measure the size of mature leaves with a CL-203 laser area meter (CID, USA). The root system, plant height, and stem diameter were scanned using a WinRhizo Pro LA2400 system (Regent, Canada). Stomatal areas were measured in fresh fully developed and healthy adult leaf portions and observed under a microscope (Nikon Eclipse Ni-U, Japan).
6
Organ Weight and Index Calculation
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At the end of the treatment, the body weight was recorded. And all the organs were removed from the attached adipose and weighted with an electronic balance (Sartorius, Germany). The organ index (mg/g) was calculated as the following formula:
7
Rat Weight Monitoring Protocol
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Each rat was measured on days 1, 3, 7, and 14 using an electronic balance (Sartorius Co., Gottingen, Lower Saxony, Germany).
8
Sensorimotor Assessment of Exosome Effects in Rats
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To evaluate neurological behavioral function in rats including the sensory and motor function, corner tests and forelimb placement tests were performed on days 1, 3, and 7 after the exosomes were administered. Briefly, for the corner test, rats were approached in a 30 ° corner and had to turn to the left or right to exit the corner. We recorded the choice of turning side for ten trials per rat. Forelimb placement tests were performed as described in the previous study (Xi et al., 2018). Each rat was tested for ten trials. The percentage of trials in which the rat placed the appropriate forelimb on the edge of the countertop in response to vibrissae stimulation was calculated. Rats were anesthetized using 50 mg/kg 2% sodium pentobarbital (Cat# P3761, Sigma) via intraperitoneal injection, and they were then sacrificed by decapitation. Ipsilateral hemisphere tissue was dissected on day 3 and weighed immediately on an electronic balance (Sartorius, Gottingen, Niedersachsen, Germany) to determine the brain wet weight. The tissue was then heated at 100°C for 1 day to determine the dry weight. The water content (%) was obtained as (wet weight – dry weight) / wet weight × 100.
9
Measuring Thousand Seed Weight
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Thousand seed weight (TSW) was determined by weighing eight replicates of 1,000 seeds to the nearest 0.0001 g using a Sartorius electronic balance.
10
Isolation of Alveolar BMSCs from Implant Debris
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In this study, human alveolar BMSCs were isolated from wasted bone debris from the implant sockets of patients who underwent oral implantation. An informed consent form for the study was signed by each patient before surgery. The study was approved by the Ethics Committee of Beijing Stomatological Hospital, Capital Medical University (ethics approval: CMUSH-IRB-KJ-PJ-2017-01), and was performed in accordance with the ethical standards laid down in the 1964 Declaration of Helsinki and its later amendments.
The primary BMSC culture method used in this study was similar to that described in our previous study [22 (link)]. During oral implant surgery, the implant sockets were prepared using a low-speed drilling technique (50 rpm, without irrigation), and the bone debris was then collected from the drill and placed in sterile tubes containing 0.5 ml phosphate-buffered saline (PBS) (HyClone, USA). An electronic balance (Sartorius, Germany) was used to weigh the tubes before and after the addition of the bone debris to enable quantification. After centrifugation, the bone debris was transferred into a 60 mm Petri dish (Corning, USA) with 5 ml of mesenchymal stem cell medium (MSCM) (ScienCell, USA) and placed in a 37°C and 5% CO2 incubator for 7 d. Thereafter, the medium was replaced every 3 d.
The primary BMSC culture method used in this study was similar to that described in our previous study [22 (link)]. During oral implant surgery, the implant sockets were prepared using a low-speed drilling technique (50 rpm, without irrigation), and the bone debris was then collected from the drill and placed in sterile tubes containing 0.5 ml phosphate-buffered saline (PBS) (HyClone, USA). An electronic balance (Sartorius, Germany) was used to weigh the tubes before and after the addition of the bone debris to enable quantification. After centrifugation, the bone debris was transferred into a 60 mm Petri dish (Corning, USA) with 5 ml of mesenchymal stem cell medium (MSCM) (ScienCell, USA) and placed in a 37°C and 5% CO2 incubator for 7 d. Thereafter, the medium was replaced every 3 d.
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