ChIP assays were described earlier [23 (link), 43 (link)]. The following antibodies were used to precipitate DREAM, MMB, and E2F complex components: E2F4 (C-20, sc-866; Santa Cruz Biotech.) [44 (link)], E2F1 (C-20, sc-193; Santa Cruz Biotech.), E2F2 (C-20, sc-633; Santa Cruz Biotech.), E2F3 (C-18, sc-878; Santa Cruz Biotech.), LIN37 (T1, custom-made at Pineda Antikörper-Service, Berlin, Germany), BMYB (N-19, Santa Cruz Biotech.), FOXM1 (C-20, sc-502, Santa Cruz Biotech.) [33 (link)], and NF-YA (G-2, sc-17753; Santa Cruz Biotech.). The E2F3 antibody was directed against the C terminus of the protein and did not distinguish between E2F3A and E2F3B, which differ in their N-terminal domain [45 (link)]. As a negative control for the ChIP experiments, rabbit IgG was used (Rabbit IgG Isotype control, No. 02-6102; ThermoFisher Scientific).
Lin37
Manufactured by Santa Cruz Biotechnology
Sourced in Germany
LIN37 is a laboratory equipment product offered by Santa Cruz Biotechnology. It is a device designed for specific laboratory applications. The core function of LIN37 is to facilitate certain experimental procedures, but further details on its intended use are not available in this factual and unbiased description.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using lin37
1
ChIP Assays for DREAM, MMB, and E2F Complexes
2
Antibody Panel for DNA Damage Signaling
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The following antibodies were used for western blot analysis: 53BP1 (Bethyl Laboratories, A300-272A, 1:3000), LIN37 (Santa Cruz Biotechnology, sc-515686, 1:200), BLM (Bethyl Laboratories, A300-572A, 1:2000), BRCA1 for mouse (R and D Systems, gift from Dr. Andre Nussenzweig, NCI, 1:1000) (Zong et al., 2019 (link)), BRCA1 for human (Millipore Sigma, 07-434, 1:1000), RAD51 (Millipore Sigma, ABE257, 1:2000), BARD1 (Thermo Fisher Scientific, PA5-85707, 1:1000), CtIP (gift from Dr. Richard Baer, [Columbia University, New York], 1:1000), MRE11 (Novus Biologicals, NB100-142, 1:2000), RIF1 (Abcam, ab13422, 1:500), SHLD1/C20orf196 (Thermo Fisher Scientific, PA5-559280, 1:200), GAPDH (Sigma, G8795, 1:10,000), KAP1 (Genetex, GTX102226, 1:2000), FANCD2 (R and D Systems, MAB93691, 1:1000), BRCA2 for human (Proteintech, 19791-1-AP, 1:500), Rb1 (Thermo Fisher Scientific, LF-MA0173, 1:1000), Phospho-Rb (Ser780) (Cell Signaling Technology, 8180T, 1:1000), Phospho-Rb (Ser807/811) (Cell Signaling Technology, 8516T, 1:1000), PCNA (Bethyl Laboratories, A300-276A, 1:3000), CDK4: (Novus Biologicals, NBP1-31308, 1:1000), CDK4 (phosphor Thr 172) (GeneTex, GTX00778, 1:1000), and RPA (Cell Signaling Technology, 2208S, 1:1000).
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