Origin 7.5 sr6

Forty-five days after induction of SE, rats received IP injections of phenobarbital (15 mg/kg), bumetanide (30 mg/kg), or a vehicle (Co). The phenobarbital was diluted in 0.9% normal saline and the bumetanide was dissolved in NaOH 0.1 M and 0.9% normal saline. In addition to the vehicle group, three treatment groups were tested: Phenobarbital alone, bumetanide alone, and a combination of phenobarbital with bumetanide.
Rats were videotaped and their behavior was reviewed and scored by a study-blind investigator for severity, frequency, and duration of tonic-clonic seizures. Origin 7.5 SR6 (Microcal Software; USA) was used for data acquisition and analyses.

To evaluate the frequency and duration of spontaneous recurrent seizures (SRS), the rats were videotaped and then reviewed by an investigator blinded to treatment groups to score the number and cumulative duration of tonic-clonic seizures. Origin 7.5 SR6 (Microcal Software, Northampton, MA, USA) software was used for data recording and analysis. Power spectrum analysis was performed after applying a Hamming window function[39 (link)].

All statistical analyses on PCR results were performed with SSPS version 19.0 (SPSS; USA). Oneway Analysis of Variance (ANOVA) with Dunnett post hoc was used to examine significant differences between groups. All results were expressed as Mean±SD.
For monitoring the data, repeated measures and the post hoc Tukey test were used for hour-to-hour and point-to-point analysis of seizure duration and mean seizure score of hour-to-hour severity for the two groups. Two-way ANOVA and post hoc Tukey were used to examine means for seizure attack frequency and duration in 20 hours. All results were presented as Mean±Standard Error of Mean (SEM). For all tests, P<0.05 was considered statistically significant. Origin 7.5 SR6 (Microcal Software, USA) was used for data acquisition and analysis.