Nicolet is50 ftir spectrometer

Manufactured by Thermo Fisher Scientific

Sourced in United States, Germany, Italy, Japan, United Kingdom

The Nicolet iS50 FTIR spectrometer is a Fourier transform infrared spectrometer designed for laboratory use. It is capable of analyzing the infrared absorption spectrum of a sample to identify its chemical composition and molecular structure.

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310 protocols using nicolet is50 ftir spectrometer

FTIR and FT-Raman Analysis of E. cava

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The IR spectrum of E. cava was recorded in the 4000–400 cm⁻¹ range using a Thermo Scientific (Waltham, MA, USA) Nicolet iS50 FTIR spectrometer. FT-Raman spectra were recorded (512 scans/spectrum) on a Thermo Scientific Nicolet iS50 FTIR spectrometer equipped with an iS50 Raman Module using an Nd:YAG laser with an excitation wavelength of 1064 nm operating at 500 mW and an InGaAs detector.
The FTIR spectral analysis of E. cava samples was performed over a spectral range of 400–4000 cm⁻¹, with a resolution of 4 cm⁻¹. Each spectrum was constructed from 16 scans in the absorbance mode, recording over the 3500–500 cm⁻¹ region.

Kim Y.S., Kim K.A., Seo H.Y., Kim S.H, & Lee H.M. (2024). Antioxidant and anti-hepatitis A virus activities of Ecklonia cava Kjellman extracts. Heliyon, 10(3), e25600.

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FTIR Analysis of Gluten Protein Structure

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The secondary structure of gluten protein was studied using Fourier
transform infrared (FTIR) spectroscopy according to the method of
Nawrocka et al.^{42 (link)} FTIR spectra were recorded
in the wavelength range of 4000–400 cm^–1 using
a Nicolet IS50 FTIR spectrometer (Thermo Nicolet Corp.). Gluten protein
sample (1 mg) was mixed with 150 mg of KBr powder and compressed at
a force of 5 kN for 30 s. FTIR spectra were recorded with 64 scans.
The secondary structure contents of the samples were analyzed using
OMNIC software package and Peakfit software (Origin, version 9.1).

Cui T., Wu T., Liu R., Sui W., Wang S, & Zhang M. (2019). Effect of Degree of Konjac Glucomannan Enzymatic Hydrolysis on the Physicochemical Characteristic of Gluten and Dough. ACS Omega, 4(6), 9654-9663.

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FTIR Analysis of Gluten Protein Structure

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The study on secondary structure of gluten proteins with different proportions of KGM at different frozen storage times was operated with Fourier transform infrared (FTIR) spectroscopy. FTIR spectra were recorded over the wavelength range of 4000 cm⁻¹ to 400 cm⁻¹ using a Nicolet IS50 FTIR spectrometer (Thermo Nicolet Corp, Madison, WI, USA) equipped with a single-reflection diamond attenuated total reflection (ATR) crystal and a mercury-cadmium-telluride (MCT) detector. A total of 1.0 mg of gluten protein sample was mixed with 150 mg KBr powder and compressed into discs at a force of 5 KN for 30 s. FTIR spectra were recorded with 64 scans and a 4 cm⁻¹ resolution against the background. The secondary structure of the samples was analyzed by the OMNIC software package (version 8.0, Thermo Nicolet Corp, Madison, WI, USA) and Origin software (version 9.1).

Cui T., Liu R., Wu T., Sui W, & Zhang M. (2019). Influence of Konjac Glucomannan and Frozen Storage on Rheological and Tensile Properties of Frozen Dough. Polymers, 11(5), 794.

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FT-IR Analysis of β-Glucan Fermentation

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The Fourier transform infrared (FT-IR) spectra of β-glucan with different fermentation times were measured using the KBr disk method on a Nicolet IS50 FT-IR spectrometer (Thermo Nicolet Co., USA) in the frequency range of 450–4000 cm⁻¹ with KBr pellets and referenced against air.

Zhang J., Wang P., Tan C., Zhao Y., Zhu Y., Bai J., Xiao X., Zhang L., Teng D., Tian J., Liu L, & Zhang H. (2022). Effects of L.plantarum dy-1 fermentation time on the characteristic structure and antioxidant activity of barley β-glucan in vitro. Current Research in Food Science, 5, 125-130.

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FTIR Spectroscopy of Lyophilized Samples

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Fourier transform infrared spectroscopy was measured using a Nicolet iS50 FTIR spectrometer (Thermo Nicolet Co. ltd., Germany) according to the method of Wang et al. [26] (link). The lyophilized samples were mixed with KBr and pressed to a disk. The mixed samples were scanned in the wavenumber range of 4000–400 cm⁻¹, and with a resolution of 4 cm⁻¹.

Hu A, & Li L. (2022). Effects of ultrasound pretreatment on functional property, antioxidant activity, and digestibility of soy protein isolate nanofibrils. Ultrasonics Sonochemistry, 90, 106193.

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Analyzing Liposome Chemical Bonds by FTIR

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The study on chemical bond of liposomes was operated with Fourier transform infrared (FTIR) spectroscopy. FTIR spectra were recorded over the wavelength range of 4000 to 400 cm⁻¹, by using a Nicolet IS50 FTIR spectrometer (Thermo Nicolet Corp, Madison, WI, USA) equipped with a single reflection diamond attenuated total reflectance (ATR) crystal and a mercury–cadmium–telluride (MCT) detector. A total of 1.0 mg of liposomes (freeze-dried samples) was mixed with 150 mg KBr powder and compressed into discs at a force of 5 KN for 30 s. FTIR spectra were recorded with 64 scans and a 4 cm⁻¹ resolution against the background. The differences between chemical bond of liposomes samples were analyzed by the FTIR spectra.

Cui T., Jia A., Yao M., Zhang M., Sun C., Shi Y., Liu X., Sun J, & Liu C. (2021). Characterization and Caco-2 Cell Transport Assay of Chito-Oligosaccharides Nano-Liposomes Based on Layer-by-Layer Coated. Molecules, 26(14), 4144.

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ATR-FTIR Spectroscopy of Samples

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Attenuated total reflectance (ATR)-FTIR spectra were recorded with
a Thermo Fisher Scientific Nicolet iS50 FTIR Spectrometer in the mid-IR
range, subtracting the contribution from air (blank), and collecting
32 scans for each spectrum with a resolution of 4 cm^–1.

Seggio M., Arcadio F., Radicchi E., Cennamo N., Zeni L, & Bossi A.M. (2024). Toward Nano- and Microplastic Sensors: Identification of Nano- and Microplastic Particles via Artificial Intelligence Combined with a Plasmonic Probe Functionalized with an Estrogen Receptor. ACS Omega, 9(17), 18984-18994.

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Fourier Transform Infrared Analysis of Nanocomposite Films

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By using Fourier transform infrared spectroscopy (FTIR) in the wavenumber range 4000–400 cm⁻¹ we investigated the presence of certain functional groups and the interactions between some components of the nanocomposite films. A Nicolet iS50 FTIR spectrometer (Thermo Fisher Scientific Inc., Waltham, MA, USA), equipped with a DTGS detector, was used to record the spectra. We used a resolution of 4 cm⁻¹, and each spectra was obtained by averaging 32 scans.
FTIR 2D maps were recorded with a Nicolet iS50R FTIR microscope (Thermo Fisher Scientific Inc., Waltham, MA, USA), with DTGS detector, in the wavenumber range 4000–600 cm⁻¹. The 2D FTIR maps were used to obtain information about the spatial distribution of the components

Motelica L., Ficai D., Oprea O., Ficai A., Trusca R.D., Andronescu E, & Holban A.M. (2021). Biodegradable Alginate Films with ZnO Nanoparticles and Citronella Essential Oil—A Novel Antimicrobial Structure. Pharmaceutics, 13(7), 1020.

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Multimodal Characterization of Nanomaterials

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Transmission electron microscopy (TEM) images were acquired by a Hitachi HT7800 electron microscopy operating at 120.0 kV. Size distribution and zeta potential were evaluated by a Malvern Zetasizer Nano ZS90 equipment. Thermogravimetric analysis was performed on a STA 449C simultaneous thermal analyzer (Netzsch, Germany). X-ray powder diffraction (XRD) patterns were recorded on a D8 Advance diffractometer (Bruker, Germany). Fourier transform infrared (FTIR) spectra were collected on a Thermo Nicolet iS50 FTIR spectrometer. The X-ray photoelectronic spectroscopy (XPS) analysis was performed using a Thermo Scientific K-Alpha spectrophotometer. The contents of released Cu and Mn elements were measured by an inductively coupled plasma instrument (ICP-OES, PerkinElmer Optima 5300 DV). UV-vis absorption spectra were recorded by a spectrophotometer (UV-3600, Shimadzu, Japan). Electron spin resonance (ESR) spectra were performed by an EMXplus spectrometer (Bruker, Germany). The fluorescence experiments were performed with a Hitachi F-7000 fluorescence apparatus at room temperature.

Zhang Y., Hu H., Deng X., Song Q., Xing X., Liu W, & Zhang Y. (2022). Cascade-Enhanced Catalytic Nanocomposite with Glutathione Depletion and Respiration Inhibition for Effective Starving-Chemodynamic Therapy Against Hypoxic Tumor. International Journal of Nanomedicine, 17, 5491-5510.

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FTIR Spectral Analysis of Solid Samples

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Fourier-transform infrared (FTIR) spectra were measured with a Nicolet iS50 FT-IR spectrometer (Thermo Scientific, USA) equipped with a DTGS detector and Omnic 9.0 software. The spectrum was collected in the middle region from 4000 to 400 cm⁻¹ at a resolution of 4 cm⁻¹, with 64 scans. The Diamond ATR accessory for solid-state measurements was used.

Matulova M., Feckova L., Novakova R., Mingyar E., Csolleiova D., Zduriencikova M., Sedlak J., Patoprsty V., Sasinkova V., Uhliarikova I., Sevcikova B., Rezuchova B., Homerova D, & Kormanec J. (2019). A Structural Analysis of the Angucycline-Like Antibiotic Auricin from Streptomyces lavendulae Subsp. Lavendulae CCM 3239 Revealed Its High Similarity to Griseusins. Antibiotics, 8(3), 102.

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