B6 jgptgsdmeem8cd566 gpt

All mice used in this study were purchased from GemPharmatech (Nanjing, Jiangsu, China) including C57BL/6JGpt mice (wild type, WT), B6/JGptGsdme^em8Cd566/Gpt (Gsdme^−/− mice), Krt14^Cre/+-Gsdme^fl/fl (keratinocyte-specific Gsdme cKO mice) and their littermate controls (Krt14 + /+-Gsdme^fl/fl). All mice in experiments were between 6-8 weeks of age and weighted 15-25 g. All mice in our experiment were male mice. All mice were randomly allocated to different groups. All mice were applied with imiquimod cream (Sichuan MingXin Pharmaceutical Co. China) on dorsal skin to establish a psoriasis-like dermatitis model. All mice were sacrificed after IMQ applied for 5 consecutive days, and their tissues were collected. No mice were excluded from the analysis. The severity of skin lesions in mice was evaluated by PASI scores, as detailed in our previous study [9 (link)]. Two investigators conducted a blind assessment of the severity of skin lesions in mice.

C57BL/6JGpt mice (WT), Gsdme^−/− (Gsdme KO) mice (B6/JGpt-Gsdme^em8Cd566/Gpt), B6/JGpt-Gsdme^em1Cflox/Gpt and B6/JGpt-H11^{em1Cin(hKRT14-iCre)}/Gpt mice were purchased from GemPharmatech (Nanjing, Jiangsu, China). Keratinocyte-specific Gsdme cKO mice (Krt14^Cre/+-Gsdme^flox/flox, GemPharmatech) and control mice (Krt14^+/+-Gsdme^flox/flox, GemPharmatech) were offspring mice through intercrossing from B6/JGpt-Gsdme^em1Cflox/Gpt and B6/JGpt-H11^{em1Cin(hKRT14-iCre)}/Gpt mice. All mice used were 5–8 weeks of age and of 15–25 g. Mice were randomly allocated to experimental groups. At 24 h before UVB exposure, we shaved the dorsal skin of all mice. For inducing acute skin inflammation, mice were exposed to 430 mJ/cm² UVB, as described previously [10 (link)]. All mice were sacrificed at indicated times after UVB exposure, and dorsal skin samples were collected. Skin samples were immersed into 2 mg/ml dispase II (Sigma-Aldrich, St. Louis, MO, USA) to separate the epidermis. We obtained approval of animal studies from Medical Ethics Committee in the Institute of Dermatology, Chinese Academy of Medical Sciences (2019-DW-002), and the Institutional Animal Care and Use Committee at Nanjing Medical University (IACUC-2101043).