Tariquidar

Manufactured by MedChemExpress

Sourced in United States, Germany

Tariquidar is a laboratory product manufactured by MedChemExpress, a leading provider of high-quality research chemicals. It is a small molecule that functions as a potent and selective inhibitor of P-glycoprotein (P-gp), a membrane transporter protein involved in the efflux of various substances from cells. Tariquidar is commonly used in research applications to study the role of P-gp in biological processes and to investigate its potential as a therapeutic target.

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Lab products found in correlation

Methyl thiazolyl tetrazolium (mtt), by Merck Group (5 mentions) Complete easypacks protease inhibitors cocktail, by Roche (4 mentions) Mitoxantrone, by Merck Group (3 mentions) Calcein am, by Calibre Scientific (3 mentions) Annexin 5 fitc, by BD (2 mentions) Phosphostop easypacks phosphotase inhibitors cocktail, by Roche (2 mentions) Elacridar, by Selleck Chemicals (2 mentions) Pf 3758309, by Apexbio (2 mentions) Gedatolisib, by Apexbio (2 mentions) Ks176, by MedChemExpress (2 mentions) Mln4924, by Chemietek (2 mentions) Thz531, by Cayman Chemical (2 mentions) Cudc 101, by MedChemExpress (2 mentions) Ko143, by Cayman Chemical (2 mentions) Mln7243, by Chemietek (2 mentions) Bisantrene, by Merck Group (1 mentions) Vinblastine, by Merck Group (1 mentions) Tetramethylrhodamine ethyl ester, by Thermo Fisher Scientific (1 mentions) Romidepsin, by Selleck Chemicals (1 mentions) Doxorubicin, by Merck Group (1 mentions)

11 protocols using tariquidar

Tariquidar-mediated P-gp Inhibition for [11C]Verapamil PET

Cited 1 time

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Tariquidar (MedChemExpress, New
Jersey, USA) was used as a P-gp inhibitor.^{24 (link),29 (link),36 (link)} Tariquidar solution for intravenous
injection was prepared as previously described,^{37 (link)} with 11 mg of Tariquidar methanesulfonate hydrate suspended
in 3 mL of saline solution, and the injection volume was adjusted
to the weight of each monkey (8 mg/kg of body weight). Radiosynthesis
and quality control of (R)-[¹¹C]verapamil
were performed as described previously.^{21 (link)}

García-Varela L., García D.V., Kakiuchi T., Ohba H., Nishiyama S., Tago T., Elsinga P.H., Tsukada H., Colabufo N.A., Dierckx R.A., van Waarde A., Toyohara J., Boellaard R, & Luurtsema G. (2020). Pharmacokinetic Modeling of (R)-[11C]verapamil to Measure the P-Glycoprotein Function in Nonhuman Primates. Molecular Pharmaceutics, 18(1), 416-428.

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Evaluation of Multidrug Resistance Modulators

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Bisantrene, doxorubicin, mitoxantrone, paclitaxel, rhodamine 123, vinblastine and verapamil were purchased from Sigma-Aldrich (St. Louis, MO). BODIPY FL-ethylenediamine (EDA), BODIPY-prazosin, BODIPY-vinblastine, calcein-AM, and tetramethylrhodamine ethyl ester (TMRE) were obtained from Invitrogen/Life Technologies (Carlsbad, CA). Laser dye styryl 751 (LDS 751) was purchased from Santa Cruz Biotechnology (Dallas, TX). Flutax was from Tocris Bioscience (Minneapolis, MN). AT9283, KW2478 and valspodar were from Apex Biotechnology (Houston, TX). YM-155, VX-680, and 17-AAG were purchased from ChemieTek (Indianapolis, IN). Romidepsin was obtained from Selleck Chem (Houston, TX). Elacridar and tariquidar were from MedChemExpress (Monmouth Junction, NJ).

Robey R.W., Robinson A.N., Ali-Rahmani F., Huff L.M., Lusvarghi S., Vahedi S., Hotz J.M., Warner A.C., Butcher D., Matta J., Edmondson E.F., Lee T.D., Roth J.S., Lee O.W., Shen M., Tanner K., Hall M.D., Ambudkar S.V, & Gottesman M.M. (2021). Characterization and tissue localization of zebrafish homologs of the human ABCB1 multidrug transporter. Scientific Reports, 11, 24150.

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Docetaxel Uptake and P-gp Inhibition

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Cells (2x10⁵) were plated in 6-well plates and left to adhere overnight in the appropriate medium. Cells were then incubated with either 2.5 nM tritium-labeled docetaxel (³H-TXT from American Radiolabeled Chemical, St. Louis, MO) or 100 nM Tariquidar (Med Chem Express, Monmouth Junction, NJ) or a combination thereof, with 5% CO₂ at 37°C. After 12 hours, the media were removed, while the adhered cells were rinsed with 1 ml PBS, treated with 0.25% trypsin, and then placed in 5 ml of scintillation fluid. The radioactivity associated with the cells was then quantified using a Beckman LS 6000 IC scintillation counter.

Edwardson D.W., Boudreau J., Mapletoft J., Lanner C., Kovala A.T, & Parissenti A.M. (2017). Inflammatory cytokine production in tumor cells upon chemotherapy drug exposure or upon selection for drug resistance. PLoS ONE, 12(9), e0183662.

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Cytotoxicity Assay with Cell Viability Markers

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MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) was purchased from Sigma (Taufkirchen, Germany), annexin-V-FITC from BD Bioscience (San Jose, CA, USA); tariquidar (p-glycoprotein inhibitor) from MedChemExpress (Monmouth Junction, NJ, USA); calcein-AM from BIOZOL (Eching, Germany); VE-822 (ATR inhibitor), LY2603618 (Chk1 inhibitor), KU-60019 (ATM inhibitor), and PV1019 (Chk2 inhibitor) from LC Laboratories (Woburn, MA, USA); Anisomycin—from NeoCorp (Weilheim, Germany). Docetaxel, and doxorubicin—from a Pharmacy of the University Hospital Hamburg-Eppendorf (Hamburg, Germany). Primary and secondary antibodies used are listed in Supplementary Table S1.

Dyshlovoy S.A., Mansour W.Y., Ramm N.A., Hauschild J., Zhidkov M.E., Kriegs M., Zielinski A., Hoffer K., Busenbender T., Glumakova K.A., Spirin P.V., Prassolov V.S., Tilki D., Graefen M., Bokemeyer C, & von Amsberg G. (2024). Synthesis and new DNA targeting activity of 6- and 7-tert-butylfascaplysins. Scientific Reports, 14, 11788.

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Cytotoxicity Evaluation Methodology

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Calcein-AM was purchased from BIOZOL (Eching, Germany); tariquidar - from MedChemExpress (Monmouth Junction, NJ, USA); MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) - from Sigma (Taufkirchen, Germany); cOmplete™ EASYpacks protease inhibitors cocktail and PhosphoSTOP™ EASYpacks - from Roche (Mannheim, Germany); docetaxel and cisplatin - from the Pharmaceutical Department of the University Hospital Hamburg-Eppendorf (Hamburg, Germany). Primary and secondary antibodies used are listed in Supplementary Table S1.

Dyshlovoy S.A., Shubina L.K., Makarieva T.N., Hauschild J., Strewinsky N., Guzii A.G., Menshov A.S., Popov R.S., Grebnev B.B., Busenbender T., Oh-Hohenhorst S.J., Maurer T., Tilki D., Graefen M., Bokemeyer C., Stonik V.A, & von Amsberg G. (2022). New diterpenes from the marine sponge Spongionella sp. overcome drug resistance in prostate cancer by inhibition of P-glycoprotein. Scientific Reports, 12, 13570.

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Investigating Cellular Signaling in Drug Response

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The following reagents and antibodies were used for biological experiments: anisomycin (NeoCorp, Weilheim, Germany); annexin-V-FITC (BD Bioscience, San Jose, CA, USA); docetaxel (Pharmacy of the University Hospital Hamburg-Eppendorf, Hamburg, Germany); SP600125 (JNK1/2 inhibitor) and SB203580 (p38 inhibitor) (LC Laboratories, Woburn, MA, USA); PD98059 (MEK inhibitor) (Merck Chemicals GmbH, Darmstadt, Germany); PhosphoSTOP™ EASYpacks phosphotase inhibitors cocktail and cOmplete™ EASYpacks protease inhibitors cocktail (Roche, Mannheim, Germany); propidium iodide (PI), MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (Sigma, Taufkirchen, Germany); RNase (Carl Roth, Karlsruhe, Germany); tariquidar (p-glycoprotein inhibitor) (MedChemExpress, Monmouth Junction, NJ, USA); primary and secondary antibodies used are listed in Table 2.

Dyshlovoy S.A., Busenbender T., Hauschild J., Girich E.V., Kriegs M., Hoffer K., Graefen M., Yurchenko A.N., Bokemeyer C, & von Amsberg G. (2022). Cytotoxic N-Methylpretrichodermamide B Reveals Anticancer Activity and Inhibits P-Glycoprotein in Drug-Resistant Prostate Cancer Cells. Marine Drugs, 20(10), 597.

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Cytotoxicity Assay with Calcein-AM

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Calcein-AM was purchased from BIOZOL (Eching, Germany), Tariquidar was purchased from MedChemExpress (Monmouth Junction, NJ, USA), MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) was purchased from Sigma (Taufkirchen, Germany) and docetaxel was purchased from a Pharmacy of the University Medical Center Hamburg-Eppendorf (Hamburg, Germany).

Leshchenko E.V., Antonov A.S., Borkunov G.V., Hauschild J., Zhuravleva O.I., Khudyakova Y.V., Menshov A.S., Popov R.S., Kim N.Y., Graefen M., Bokemeyer C., von Amsberg G., Yurchenko A.N, & Dyshlovoy S.A. (2023). New Bioactive β-Resorcylic Acid Derivatives from the Alga-Derived Fungus Penicillium antarcticum KMM 4685. Marine Drugs, 21(3), 178.

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Reagents for Biological Experiments

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The following reagents were used for biological experiments: RNase (Carl Roth, Karlsruhe, Germany); PhosSTOP™ EASYpachosphatasease inhibitors cocktail and cOmplete™ EASYpacks protease inhibitors cocktail (Roche, Mannheim, Germany); MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) (Sigma, Taufkirchen, Germany); Enzalutamide (Hycultec GmbH, Beutelsbach, Germany); Tariquidar (MedChemExpress, Monmouth Junction, NJ, USA). Primary and secondary antibodies used are listed in Table 2.

Dyshlovoy S.A., Zhuravleva O.I., Hauschild J., Busenbender T., Pelageev D.N., Yurchenko A.N., Khudyakova Y.V., Antonov A.S., Graefen M., Bokemeyer C, & von Amsberg G. (2023). New Marine Fungal Deoxy-14,15-Dehydroisoaustamide Resensitizes Prostate Cancer Cells to Enzalutamide. Marine Drugs, 21(1), 54.

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Docetaxel-based chemotherapy protocol

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Docetaxel was purchased from Pharmacy of the University Hospital Hamburg-Eppendorf (Hamburg, Germany); PhosphoSTOP™ EASYpacks phosphotase inhibitors cocktail and cOmplete™ EASYpacks protease inhibitors cocktail were purchased from Roche (Mannheim, Germany); MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) was purchased from Sigma (Taufkirchen, Germany); 3-Methyladenine was purchased from Enzo Life Sciences (Farmingdale, NY, USA); Tariquidar was purchased from MedChemExpress (Monmouth Junction, NJ, USA); Primary and secondary antibodies used for Western blotting are listed in Table 2.

Dyshlovoy S.A., Shubina L.K., Makarieva T.N., Guzii A.G., Hauschild J., Strewinsky N., Berdyshev D.V., Kudryashova E.K., Menshov A.S., Popov R.S., Dmitrenok P.S., Graefen M., Bokemeyer C, & von Amsberg G. (2022). New Guanidine Alkaloids Batzelladines O and P from the Marine Sponge Monanchora pulchra Induce Apoptosis and Autophagy in Prostate Cancer Cells. Marine Drugs, 20(12), 738.

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Detailed Compound Sourcing Protocol

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Mitoxantrone was obtained from Sigma-Aldrich (St. Louis, MO). Pheophorbide a was from Frontier Specialty Chemicals (Logan, UT). MLN-7243 and MLN-4924 were purchased from ChemieTek (Indianapolis, IN). THZ531 and Ko143 were obtained from Cayman Chemical (AnnArbor, MI). PF-3758309 and Gedatolisib were from ApexBio Technology (Houston, TX). CUDC-101, KS176 and tariquidar were from MedChem Express (Monmouth Junction, NJ). Elacridar was from Selleck chemicals (Houston, TX).

Thomas J.R., Frye W.J., Robey R.W., Warner A.C., Butcher D., Matta J.L., Morgan T.C., Edmondson E.F., Salazar P.B., Ambudkar S.V, & Gottesman M.M. (2024). Abcg2a is the functional homolog of human ABCG2 expressed at the zebrafish blood–brain barrier. Fluids and Barriers of the CNS, 21, 27.

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