N isopropylmethacrylamide nipmam

N-isopropylmethacrylamide (NIPMAM) and N,N’-bis(acryloyl)cystamine (BAC) were purchased from Sigma-Aldrich (St Louis, MO, USA). Methacrylic acid (MAA) was purchased from Sinopharm Chemical Reagent Co., Ltd. (Shanghai, China). Potassium persulfate (KPS) and sodium dodecyl sulfate (SDS) were purchased from Aladdin Reagent Inc. (Shanghai, China). Doxorubicin·HCl (DOX·HCl) was purchased from Meilun Biotech Co., Ltd. (Dalian, China). Indocyanine Green was purchased from J&K Scientific Ltd. (Beijing, China). Rhodamine B, 4-dimethylaminopyridine (DMAP), N,N’-Dicyclohexylcarbodiimide (DCC) and 2-hydroxyethyl methacrylate (HEMA) were purchased from Energy Chemical (Shanghai, China). Pitstop 2 was purchased from MedChemExpress (Shanghai, China). RNAprep FastPure, Goldenstar^TM RT6 cDNA Synthesis Mix and Master qPCR Mix were purchased from Tsingke Biotechnology Co., Ltd. (Beijing, China).

N-n-propylacrylamide (NNPAM) was synthesized via a Schotten–Baumann reaction published by Hirano et al. [61 (link)]. For this reaction, acryloylchloride (Sigma-Aldrich Chemie GmbH, Munich, Germany; purity 98%), n-propylamine (Fluka, Sigma-Aldrich Chemie GmbH, Munich, Germany; purity 99%), triethylamine (Grüssing GmbH Analytika, Filsum, Germany; purity 99%), and methylenechloride (p.a.) were used as received. The obtained monomer NNPAM was washed with $\mathrm{N}\mathrm{a}\mathrm{H}\mathrm{C}{\mathrm{O}}_3$ (10 wt%) and dried over $\mathrm{M}\mathrm{g}\mathrm{S}{\mathrm{O}}_4$ . After filtration, the solvent was evaporated and the product was distilled in vacuum (115 °C, 10 mbar). N-isopropylacrylamide (NIPAM; Sigma-Aldrich Chemie GmbH, Munich, Germany; purity 97%) and N-isopropylmethacrylamide (NIPMAM; Sigma-Aldrich Chemie GmbH, Munich, Germany; purity 97%) were purified by recrystallization from hexane. The cross-linker N,N’-methylenebisacrylamide (BIS; Sigma-Aldrich Chemie GmbH, Munich, Germany; purity 99%), the initiator ammonium persulfate (APS; Sigma-Aldrich Chemie GmbH, Munich, Germany; purity ≥98%), and pyrene (Sigma-Aldrich Chemie GmbH, Munich, Germany; purity ≥99%) were used without further purification. For all experiments, purified water from an Arium pro VF system (Sartorius AG, Göttingen, Germany) was used.