RIP assay was performed using the Magna RIP RNA-Binding Protein Immunoprecipitation Kit (Millipore Corp., USA). Cell extracts were harvested with RIP lysis buffer and mixed with magnetic beads and antibodies against EZH2, LSD1 and SUZ12 (Abcam, UK). With anti-IgG antibody as control, the co-precipitated RNA was purified and analyzed by qRT-PCR.
Antibodies against ezh2
Manufactured by Abcam
Sourced in United Kingdom
Antibodies against EZH2 are reagents used in research applications to detect and study the expression of the EZH2 protein. EZH2 is a histone methyltransferase that plays a role in the epigenetic regulation of gene expression. These antibodies can be used in techniques such as Western blotting, immunohistochemistry, and immunofluorescence to analyze the presence and distribution of EZH2 in various cell and tissue samples.
Automatically generated - may contain errors
Lab products found in correlation
Suz12, by Abcam (1 mentions)
Magna rip rna binding protein immunoprecipitation kit, by Merck Group (1 mentions)
Phenylmethylsulfonyl fluoride, by Roche (1 mentions)
Ripa protein extraction reagent, by Beyotime (1 mentions)
Gapdh, by Bio-Rad (1 mentions)
0.22 μm nc membranes, by Merck Group (1 mentions)
Protease inhibitor cocktail, by Roche (1 mentions)
Quantity one software, by Bio-Rad (1 mentions)
2 protocols using antibodies against ezh2
1
RIP Assay for RNA-Binding Proteins
2
Protein Expression Analysis by Western Blot
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Cells were collected after 48 hours of transfection and lysed using RIPA protein extraction reagent (Beyotime, Beijing, China) supplemented with a protease inhibitor cocktail (Roche, CA, USA) and phenylmethylsulfonyl fluoride (Roche). Cell protein lysates were separated by 10% SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and transferred onto 0.22-μm NC membranes (Sigma). The membranes were incubated with specific antibodies. Autoradiograms were quantified by densitometry (Quantity One software; Bio-Rad), and GAPDH was used as a control. The specific antibodies for GAPDH, p21 and p57 were purchased from Cell Signaling Technology, Inc. Antibodies against EZH2 were purchased from Abcam.
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