Gemini c18

Manufactured by Phenomenex

Sourced in United States, Italy, Japan, Germany

The Gemini C18 is a reversed-phase liquid chromatography column designed for the separation and analysis of a wide range of organic compounds. It features a fully porous silica-based stationary phase with a C18 alkyl bonded ligand. The column is capable of operating at high pressure and temperature conditions, making it suitable for a variety of analytical applications.

Automatically generated - may contain errors

Lab products found in correlation

Luna c18, by Phenomenex (9 mentions) Lc 20a hplc system, by Shimadzu (7 mentions) Agilent 1200 hplc, by Agilent Technologies (5 mentions) Ultimate 3000, by Thermo Fisher Scientific (4 mentions) Methanol, by Merck Group (4 mentions) Agilent hplc system, by Agilent Technologies (3 mentions) Securityguard, by Phenomenex (3 mentions) Prominence lc 20a series, by Shimadzu (3 mentions) Spd m20a, by Shimadzu (3 mentions) Flexar, by PerkinElmer (3 mentions) Dgu 20a3, by Shimadzu (3 mentions) Affinity 1 ft ir spectrometer, by Shimadzu (2 mentions) L 2455, by Phenomenex (2 mentions) U 2900 spectrophotometer, by Hitachi (2 mentions) Av 700 mhz nmr spectrometer, by Bruker (2 mentions) Maxis 4g, by Bruker (2 mentions) Hplc system, by Waters Corporation (2 mentions) Avidin agarose beads, by Merck Group (2 mentions) Trypsin, by Promega (2 mentions) Tmt 10 plex reagent, by Thermo Fisher Scientific (2 mentions)

326 protocols using gemini c18

Synthesis and Characterization of LMP2 Peptides

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 4

1.1 General Details

Peptides LMP2 S1 (DRHSDYQPLGTQDQSLYLGLQHDGNDGL, SEQ ID NO: 5) and LMP2 S2 (SLYLGLQHDGNDGLPPPPYSPRDDSSQHIYEEA, SEQ ID NO: 10) were synthesised essentially as described above. The peptides were then purified (>95%) by RP-HPLC and their identities confirmed by analytical RP-HPLC and mass spectrometry.

Results

FIG. 1 shows an RP-HPLC trace of LMP2 S1, under the following conditions: column: Phenomenex Gemini C18 (5μ 110 Å, 4.6×150 mm); gradient: 0-1 min, 5% B then 5% B to 65% B over 30 min., eluting at 1 mL/min., Rt 14.4 min.

ESI-MS trace m/z [M+2H]2+=1572.7.

FIG. 2 shows an RP-HPLC trace of LMP2 S2, under the following conditions: column: Phenomenex Gemini C18 (5μ 110 Å, 4.6×150 mm); gradient: 0-1 min, 1% B then 1% B to 61% B over 30 min., eluting at 1 mL/min., Rt 16.0 min.

ESI-MS trace m/z [M+2H]2+=1448.2.

US11014960B2. Amino acid and peptide conjugates and uses thereof (2021-05-25). AUCKLAND UNISERVICES LIMITED [NZ]. Inventors: Margaret Anne Brimble [NZ], Peter Roderick Dunbar [NZ], Geoffrey Martyn Williams [NZ], Daniel Verdon [NZ].

+ Open protocol

+ Expand

Synthesis of 1-Methyl-N-tetrahydropyran-3-yl-pyrazol-4-amine and Its Sulfonamide Derivative

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 22

[Figure (not displayed)]

Step 1: 1-methyl-N-tetrahydropyran-3-yl-pyrazol-4-amine. General procedure A was followed using 1-methylpyrazol-4-amine and tetrahydropyran-3-one. The crude product was purified by prep-HPLC (column: Phenomenex Gemini C18 10 μm 250×50 mm; mobile phase: [water (10 mM NH₄HCO₃)-ACN]; B: 0-30%, 21 min) to give the title compound as a red oil. ¹H NMR (400 MHz, chloroform-d) δ=7.12 (s, 1H), 6.93 (s, 1H), 3.92 (d, J=11 Hz, 1H), 3.81 (s, 3H), 3.77-3.68 (m, 1H), 3.59-3.48 (m, 1H), 3.38-3.30 (m, 1H), 3.12-3.01 (br. s, 1H), 2.04-1.92 (m, 1H), 1.80-1.68 (m, 1H), 1.67-1.48 (m, 2H).

Step 2:3-(1,2,3,5,6,7-hexahydro-s-indacen-4-yl)-1-[(1-methyl-1H-pyrazol-4-yl)(oxan-3-yl)sulfamoyl]urea sodium salt. General procedure B was followed using {[(1,2,3,5,6,7-hexahydro-s-indacen-4-yl)carbamoyl]amino}sulfonyl chloride and 1-methyl-N-tetrahydropyran-3-yl-pyrazol-4-amine. The crude product was purified by prep-HPLC (column: Phenomenex Gemini C18 10 μm 250×50 mm; mobile phase: [water (10 mM NH₄HCO₃)-ACN]; B: 7-37%, 21 min) to give the title compound as a white solid. ¹H NMR (400 MHz, MeOD) δ=7.68 (s, 1H), 7.40 (s, 1H), 6.98 (s, 1H), 4.26-4.13 (m, 1H), 4.12-4.06 m, 1H), 3.88 (s, 3H), 3.79-3.71 (m, 1H), 3.13-3.04 (m, 2H), 2.88 (t, J=8 Hz, 4H), 2.80 (t, J=7 Hz, 4H), 2.12-2.04 (m, 5H), 1.79-1.60 (m, 2H), 1.39-1.26 (m, 1H).

LC-MS (ESI): m/z: [M+H]⁺=460.1

US11518757B2. Sulphonyl urea derivatives as NLRP3 inflammasome modulators (2022-12-06). NodThera Limited [GB]. Inventors: David Harrison [GB], Alan Paul Watt [GB], Mark G. Bock [US].

+ Open protocol

+ Expand

Synthesis and Characterization of LMP2 Peptides

Check if the same lab product or an alternative is used in the 5 most similar protocols

Example 4

1.1 General Details

Results

ESI-MS trace m/z [M+2H]2+=1572.7

ESI-MS trace m/z [M+2H]2+=1448.2

US10253062B2. Amino acid and peptide conjugates and uses thereof (2019-04-09). None [NZ], None [NZ], None [NZ], None [NZ]. Inventors: Margaret Anne Brimble [NZ], Peter Roderick Dunbar [NZ], Geoffrey Martyn Williams [NZ], Daniel Verdon [NZ].

+ Open protocol

+ Expand

Quantitative Analysis of Tomato Polyphenols and Carotenoids

Check if the same lab product or an alternative is used in the 5 most similar protocols

Quantitative analyses were carried out on a Ultimate3000 "UHPLC focussed" instrument equipped with a binary high pressure pump, a Photodiode Array detector, a Thermostatted Column Compartment, and an Automated Sample Injector (Thermo Scientific, Milano, Italy). Collected data were processed through a Chromeleon Chromatography Information Management System v. 6.80. Chromatographic runs were all performed using a reverse-phase column (Gemini C18, 250 × 4.6 mm, 5 µm particle size, Phenomenex, Bologna, Italy) equipped with a guard column (Gemini C18, 4 × 3.0 mm, 5 µm particle size, Phenomenex, Italy). The temperature was kept at 25 • C, the flow rate was 1 mL/min and the injector volume selected was 20 µL. Tomato polyphenols and carotenoids were eluted, identified and quantified according to Siracusa et al. [12] . All analyses were carried out in triplicate; results are reported in microgram (µg) of compound per gram (g) of vegetable material fresh weight (FW).

Patanè C., Siah S., Pellegrino A., Coséntino L., & Siracusa L. (2021). Fruit Yield, Polyphenols, and Carotenoids in Long Shelf-Life Tomatoes in Response to Drought Stress and Rewatering. Agronomy, 11(10), 1943-1943.

+ Open protocol

+ Expand

Analytical Standards for Steroid Hormone Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Analytical standards for 17α‐ethinylestradiol (EE2), 17β‐estradiol (E2β), diethylstilbestrol (DES), estrone (E1), estriol (E3), hexestrol (HEX), progesterone (PS), testosterone (TS), 4‐androstene‐3,17‐dione (AD), 17α‐methyltestosterone (MTS), testosterone propionate (TSP), as well as internal standards (IS) for β‐estradiol‐d2 (E2‐d2) and progesterone‐d9 (PS‐d9), were all obtained from Sigma Aldrich. Additionally, 17α‐estradiol (E2α) and 17α‐hydroxyprogesterone (HPS) were purchased from Dr. Ehrenstorfer. Dienestrol (DE) and α‐zearalanol (ZAL) were supplied through TCI EUROPE N.V., and Kanto Chemical Co., Inc, respectively. IS for diethylstilbestrol‐d8 (DES‐d8) and hexestrol‐d4 (HEX‐d4) were procured from Toronto Research Chemicals Inc. Bondesil C18 bulk sorbent and Bondesil PSA bulk sorbent were acquired from Agilent. HPLC‐grade acetonitrile and ammonia solvents were purchased from J. T. Baker, and Merck Millipore, respectively. Lastly, Waters Xbridge BEH C18, used to analyze negatively charged hormones, was obtained from Waters while Phenomenex Gemini C18, used to analyze positively charged hormones, was obtained from Phenomenex.

Li I., Yang W., Chou C., Chen Y., Kuo S, & Wang S. (2019). Analysis of steroid hormones in shell eggs from layer breeds common to Taiwan by liquid chromatography–tandem mass spectrometry. Food Science & Nutrition, 7(7), 2319-2326.

+ Open protocol

+ Expand

Comprehensive Analytical Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

Optical rotations were measured using a 341 Polarimeter (Perkin-kinelmer, Inc., Norwalk, CT, USA). The CD spectra were measured on a Chirascan circular dichroism spectrometer (Applied Photophysics, Ltd., Surrey, UK). UV spectra were measured on a U-2900 spectrophotometer (Hitachi, Tokyo, Japan). IR spectra were recorded on an Affinity-1 FT-IR spectrometer (Shimadzu, Tokyo, Japan). The 1D and 2D NMR spectra were recorded on a Bruker AV-700 MHz NMR spectrometer (Bruker Biospin GmbH, Rheinstetten, Germany) with tetramethylsilane (TMS) as the internal standard. Mass spectrometric data were obtained on a quadrupole-time-of-flight mass spectrometry (Bruker Maxis 4G) for HRESIMS. Column chromatography was performed while using silica gel (100–200 mesh, 300–400 mesh; Jiangyou Silica gel development, Inc., Yantai, China), Sephadex LH-20 (GE Healthcare Bio-Sciences AB, Uppsala, Sweden). HPLC was carried out while using a reversed-phase column (Phenomenex Gemini C18, 250 mm × 4.6 mm, 5 μm; Phenomenex, Torrance, CA, USA) with UV detection at 270 nm and 320 nm. Semi-preparative HPLC was performed on a Hitachi HPLC station (Hitachi-L2130) with a Diode Array Detector (Hitachi L-2455) using a Phenomenex ODS column (250 mm × 10.0 mm, 5 mm; Phenomenex, Torrance, CA, USA) with UV detection at 320 nm.

Liu W., Zhang W., Jin H., Zhang Q., Chen Y., Jiang X., Zhang G., Zhang L., Zhang W., She Z, & Zhang C. (2019). Genome Mining of Marine-Derived Streptomyces sp. SCSIO 40010 Leads to Cytotoxic New Polycyclic Tetramate Macrolactams. Marine Drugs, 17(12), 663.

+ Open protocol

+ Expand

LC-MS/MS Analysis of Cardiovascular Drugs

Check if the same lab product or an alternative is used in the 5 most similar protocols

The LC-MS/MS system consisted of an Agilent 1260 Infinity LC system connected with an Agilent 6460 triple quadrupole mass spectrometer (Agilent Technologies, Santa Clara, CA, USA) with an electrospray ionization source. Cardiovascular drugs were separated using a Phenomenex Gemini C18 (50 × 2.00 mm, 3micron) column (Phenomenex, Torrance, CA, USA). The column oven temperature was set to 30 °C, and the sample injection volume was 5 μL. The sample was kept at 4 °C, and the injection needle was washed with water:-acetonitrile (1:1) solution after each injection. The mobile phases consisted of water containing 0.1% formic acid (mobile phase A) and acetonitrile containing 0.1% formic acid (mobile phase B) at a flow rate of 0.200 mL/min.
The mass spectrometer was operated in positive and negative multiple reaction monitoring (MRM) mode. The source conditions were set as follows: drying gas temperature: 300 °C; dry gas flow: 5 L/min; nebulizer: 45 psi; sheath gas temperature: 350 °C; sheath gas flow: 11 L/min. Optimized MRM transitions are listed in Table 1 with dwell time and cell accelerator voltage as 100 msec and 4 V, respectively.
MassHunter Workstation Acquisition software B.07.00 (Agilent Technologies, Santa Clara, CA, USA) was used to operate the LC/MS systems, and the data were processed using Qualitative Analysis B.06.00 (Agilent Technologies, Santa Clara, CA, USA).

Kim H.M., Park J.H., Long N.P., Kim D.D, & Kwon S.W. (2019). Simultaneous determination of cardiovascular drugs in dried blood spot by liquid chromatography-tandem mass spectrometry. Journal of Food and Drug Analysis, 27(4), 906-914.

+ Open protocol

+ Expand

Purification and Analytical Techniques for Natural Products

Check if the same lab product or an alternative is used in the 5 most similar protocols

Data for specific rotation measurements were obtained on a Rudolph Research Autopol III automatic polarimeter. Column chromatography was performed using silica gel and HP20SS. Preparative HPLC was carried out on a Shimadzu system equipped with LC-6AD pumps, coupled to an SPD-M20A PDA detector, and a Phenomenex Luna C₁₈ column (21.2 × 250 mm and 10 × 250 mm, 5 μm). Analytical and semipreparative HPLC were conducted using a Waters HPLC system with 1525 binary pumps and a 2998 PDA detector using Phenomenex Gemini C₁₈ (250 × 4.6 mm, 1 mL/min, 5 μm) and Kinetex pentafluorophenyl (250 × 10 mm, 4 mL/min, 5 μm) columns. NMR data were collected on a Varian 600 MHz NMR spectrometer. Microcentrifuge-tube-based ultrafiltration filters (100 kDa) were obtained from Pall Corporation (Houston, TX, USA). Salmon sperm DNA and other chemicals were purchased from MilliporeSigma (St. Louis, MO, USA). Cephaeranthine was purchased from Cayman Chemical Company (Ann Arbor, MI, USA), and tetrandrine (16) and daurisoline (17) were obtained from MilliporeSigma. Distilled water was prepared with a Milli-Q water purification apparatus (Millipore, Bedford, MA, USA). All solvents were of ACS grade or better.

Ma H., Liang H., Cai S., O’Keefe B.R., Mooberry S.L, & Cichewicz R.H. (2020). An Integrated Strategy for the Detection, Dereplication, and Identification of DNA-Binding Biomolecules from Complex Natural Product Mixtures. Journal of natural products, 84(3), 750-761.

+ Open protocol

+ Expand

HPLC Analysis of Crude Extracts

Check if the same lab product or an alternative is used in the 5 most similar protocols

The analyses were carried out using a Thermo^® HPLC (Thermo Electron, Waltham, MA, USA), with a PDA (photo diode array) spectrophotometry detector module (Model Finnigan™ Surveyor PDA Plus Detector-Thermo Electron, Waltham, MA, USA), integral pumps, and degasser (Finnigan™ Surveyor LC Pump Plus - Thermo Electron, Waltham, MA, USA) and autosampler (Finnigan™ Surveyor Autosampler Plus-Thermo Electron, Waltham, MA, USA) equipped with a 10 µL loop and controller software (Chromquest™, version 4.2, Thermo Electron, Waltham, MA, USA), a Phenomenex^® Gemini C-18 (250 × 4.6 mm, 5 µm) (Phenomenex^®, Torrance, CA, USA), and a guard column (Phenomenex^® SecurityGuard™-RP C-18 cartridge) (Phenomenex^®, Torrance, CA, USA). It was used as a mobile phase water:formic acid (pH 2.5) (phase A) and acetonitrile:formic acid (pH 2.5) (phase B). It used a linear gradient of 0 min 18% B; 13 min 25% B; 16 min 34% B; 20 min 42% B; 23 min 65% B; 25 min 18% B, with a flow of 0.8 mL/min, and 100 µL injection volume. Detection was performed at 210 nm [29 (link)].
The sample preparation was carried out following a previous study using 1 g of each crude extract instead the pulverized leaves. The standard solution’s preparation and obtention of the calibration curve were conducted according to the previous study (Epicatechin calibration curve: y = 151,061x + 2,726,000) [29 (link)].

de Paula M.N., Ribeiro T.D., Isolani R., de Medeiros Araújo D.C., Borges A.S., Philippsen G.S., de Cássia Ribeiro Gonçalves R., Kitagawa R.R., Seixas F.A, & de Mello J.C. (2022). An In Vitro and In Silico Investigation about Monteverdia ilicifolia Activity against Helicobacter pylori. Antibiotics, 12(1), 46.

+ Open protocol

+ Expand

Panaxynol Extraction and UPLC Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Panaxynol was prepared at the concentration of 100 ppm in methanol (MeOH). A total of 150 mg of VG powder was extracted by sonication with 10 mL MeOH for 30 min at 40 °C. The solution was then filtered via 0.22 µm membrane filter prior to ultra performance liquid chromatography (UPLC) analysis. UPLC was performed on an ACQUITY UPLC H-Class system (Waters, Milford, MA, USA) equipped with photodiode array detector (PDA) detector (203 nm) and Phenomenex Gemini C18 (150 × 4.6 mm. i.d., 3 μm) (Phenomenex, Torrance, CA, USA) connected to Empower software. The separation was achieved with mobile phase of acetonitrile (A) and water (B) as follows: 0–8 min: 22–55% A; 8–20 min: 55–90% A; 22–27 min: 90–95% A; 27–30 min: 22% A; 30–35 min: 22% A. The column temperature was set at 30 °C. The HPLC flow rate was 0.65 mL/min. A total of 5 μL of sample solution was injected into the UPLC system.

Lee D., Lee J., Vu-Huynh K.L., Van Le T.H., Tuoi Do T.H., Hwang G.S., Park J.H., Kang K.S., Nguyen M.D, & Yamabe N. (2019). Protective Effect of Panaxynol Isolated from Panax vietnamensis against Cisplatin-Induced Renal Damage: In Vitro and In Vivo Studies. Biomolecules, 9(12), 890.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!