The presence of CD14, CD25, CD28, CD45, CD61, CD90, TLR1 and TLR4 were analyzed using a flow cytometer (CYTOMICS FC 500, Beckman Coulter, Miami, FL) according to manufacturer's instruction. Anti-CD14-PE-Cy7, CD25-FITC, TLR4-PE, CD28-PE, CD61-PE, CD90-FITC and CD45-APC were purchased from (BD Biosciences) ; anti-TLR1-PE was purchased from (eBioscience).
Anti tlr1 pe
Manufactured by Thermo Fisher Scientific
Anti-TLR1-PE is a monoclonal antibody conjugated to the fluorescent dye, R-Phycoerythrin (PE). It is designed for the detection and quantification of Toll-like Receptor 1 (TLR1) expression on the surface of cells using flow cytometry.
Automatically generated - may contain errors
Lab products found in correlation
Anti cd14 pe cy7, by BD (2 mentions)
Tlr4 pe, by BD (2 mentions)
Cd61 pe, by BD (2 mentions)
Cd25 fitc, by BD (2 mentions)
Cytomics fc500, by Beckman Coulter (2 mentions)
Cd45 apc, by BD (2 mentions)
Cd90 fitc, by BD (2 mentions)
Cd28 pe, by BD (1 mentions)
Pe cy5 anti cd4, by BD (1 mentions)
Cd3 fitc, by BD (1 mentions)
2 protocols using anti tlr1 pe
1
Multiparametric Flow Cytometry Analysis
2
Multiparametric Immune Cell Profiling
Check if the same lab product or an alternative is used in the 5 most similar protocols
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The presence of CD3, CD4, CD14, CD25, CD45, CD61, CD90, TLR1 and TLR4 were analyzed using a flow cytometer (CYTOMICS FC 500, Beckman Coulter, Miami, FL) according to manufacturer's instruction and the data was analyzed using Flow Jo analysis software. Anti-CD14-PE-Cy7, CD25-FITC, TLR4-PE, CD61-PE, CD90-FITC, CD45-APC, CD3-FITC and anti-CD4-PE-Cy5 were purchased from (BD Biosciences) ; anti-TLR1-PE was purchased from (eBioscience).
Cell cycle analysis and apoptosis analysis were performed according to the manufacturer's protocol as previously described 43 (link). All experiments were performed at least in triplicate.
Cell cycle analysis and apoptosis analysis were performed according to the manufacturer's protocol as previously described 43 (link). All experiments were performed at least in triplicate.
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