Reverse-phase high-performance liquid chromatography (HPLC) was performed on a JASCO HPLC system (JASCO Corporation, Tokyo, Japan), consisting of a PU-1580 intelligent HPLC pump, an LG-1580-04 quaternary gradient unit, a UV-1575 intelligent UV/VIS detector, a PG-1580-54 4-line degasser, and a CO-1560 intelligent column thermostat. The BORWIN chromatographic system (JMBS Developments, Le Fontanil, France) was used for HPLC data analysis. Chromatographic separation was accomplished on a Phenomenex C18 reverse-phase column (4.6×250 cm, 5 μm, Phenomenex Inc., Torrance, CA, USA) at 30°C and was monitored at 280 nm. The linear gradient solvent system consisted of 0.5% acetic acid in water (solvent A) and 100% CH3CN (solvent B), and was adjusted from 75% (solvent A):25% (solvent B) to 0% (solvent A):100% (solvent B) over 60 min at a flow rate of 0.5 mL/min.
Lg 1580 04 quaternary gradient unit
Manufactured by Jasco
Sourced in Japan, United States
The LG-1580-04 quaternary gradient unit is a laboratory equipment designed for creating precise and controlled gradient mixtures of up to four different solvents or solutions. It is a core component of liquid chromatography systems, responsible for accurately blending and delivering the mobile phase for separation and analysis of samples.
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2 protocols using lg 1580 04 quaternary gradient unit
1
Reversed-Phase HPLC Separation of Compounds
2
Quantification of Dexamethasone Release
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A modified method from United States Pharmacopeia was used to quantify the amount of dexamethasone which has been released. A Jasco high-performance liquid chromatography (HPLC) system with a PU-1580 Pump equipped with LG-1580-04 quaternary gradient unit, AS 1559 Intelligent Auto Sampler, UV 1559 intelligent UV/VIS Detector (all Jasco, Oklahoma City, USA). Purospher® Star RP-18 endcapped (5 µm) column (Merck Millipore, Billerica, Massachusetts, USA), operated at 40 °C, was used. Acetonitrile and double distilled water in a ratio 30:70 V/V were used as the mobile phase at a flow of 1 mL min−1. 20 µL of the sample were injected and analyzed at λ = 258 nm. The retention time for dexamethasone was found to be 8 min. Data recording and processing were carried out with the software ChromNAV Ver.2 (Jasco, Oklahoma City, USA).
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