The SSRI, FLX and Lipopolysaccharide (LPS) (obtained from E. coli, which carries serotype 055: B5) were obtained from Sigma-Aldrich (St. Louis, MO, USA). Enzyme-linked immunosorbent assay (ELISA) kits for corticosterone, IL-6, CRP and TNF-α, were purchased from Abcam Biotechnology, Inc. (Cambridge, UK). All the drugs were freshly prepared and injected intraperitoneally (i. p.) at a final injection volume of 5 mL/kg. The injections were administered between 8:00 AM and 10:00 AM, irrespective of the stress schedule. Rabbit polyclonal anti-phospho-JAK2 and goat anti-phospho-STAT3 (pSTAT3) were supplied by Santa Cruz Biotechnology, Inc. (Dallas, TX, USA). All the other chemicals were of analytical grade and were obtained from Sigma-Aldrich or Cell Signaling Technology (Beverly, MA, USA).
Goat anti phospho stat3 pstat3
Manufactured by Santa Cruz Biotechnology
Sourced in United States
Goat anti-phospho-STAT3 (pSTAT3) is a primary antibody used to detect the phosphorylated form of the Signal Transducer and Activator of Transcription 3 (STAT3) protein. STAT3 is a transcription factor that plays a crucial role in cellular signaling pathways. The phosphorylation of STAT3 is an important regulatory mechanism, and the Goat anti-phospho-STAT3 (pSTAT3) antibody can be used to monitor this post-translational modification.
Automatically generated - may contain errors
2 protocols using goat anti phospho stat3 pstat3
1
Investigating Stress-Induced Immune Responses
2
Investigating JAK2/STAT3 Signaling Pathway
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STZ and sitagliptin (Januvia) were purchased from Sigma-Aldrich (St Louis, MO, USA) and Merck & Co., Inc. (Whitehouse, NJ, USA), respectively. Rabbit polyclonal anti-phospho-JAK2 and goat anti-phospho-STAT3 (pSTAT3) were supplied by Santa Cruz Biotechnology Inc. (Dallas, TX, USA). All other chemicals were of analytical grade and were obtained from standard commercial supplies.
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