Anti c ebpα sc 61x

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Anti-C/EBPα (sc-61X) is a laboratory reagent produced by Santa Cruz Biotechnology. It is an antibody that recognizes the C/EBPα (CCAAT/enhancer-binding protein alpha) protein. C/EBPα is a transcription factor that plays a role in the regulation of genes involved in cell growth and differentiation.

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Lab products found in correlation

Anti pparγ sc 7196x, by Santa Cruz Biotechnology (3 mentions) Anti med1 a300 793a, by Fortis Life Sciences (2 mentions) Anti β actin a1978, by Merck Group (2 mentions) Anti med12 a300 774a, by Fortis Life Sciences (2 mentions) Anti s2p pol 2 ab5095, by Abcam (2 mentions) Z 4 hydroxytamoxifen 4oht h7904, by Merck Group (2 mentions) Anti pol 2, by Merck Group (1 mentions) Anti h3k4me3, by Merck Group (1 mentions) Anti h3k4me1, by Abcam (1 mentions) Anti h3k4me2, by Abcam (1 mentions) Anti h3k27me3, by Merck Group (1 mentions) Anti ctcf, by Merck Group (1 mentions) Anti h3k36me3, by Abcam (1 mentions) Anti c ebpβ sc150x, by Santa Cruz Biotechnology (1 mentions) Anti p300 sc 585x, by Santa Cruz Biotechnology (1 mentions) Anti h3k9me2, by Abcam (1 mentions) Anti h3k27ac ab4729, by Abcam (1 mentions)

Spelling variants of this product

C/EBPα (72 citations) Anti-C/EBPα (21 citations) C/EBPα (sc-61x) (2 citations) (sc-61X, (2 citations)

3 protocols using anti c ebpα sc 61x

Establishment of ChREBP and SREBP1a Overexpression Models

Cited 1 time

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A triple T7 (3×T7) double StrepII tag was N-terminally subcloned into retroviral vector pMSCVhyg to generate pMSCVhyg-3xT7 using a pTAG1-hygroTK-N-term 3xT7 double StrepII tag (Brown et al. 2017 (link)) as a template. Constitutive active form of mouse ChREBP or nuclear form of mouse SREBP1a was PCR-amplified using full-length clones as templates (Addgene 39235 and 32017) and was subcloned into pMSCVhyg-3xT7 to generate pMSCVhyg-3xT7-CA-ChREBP or pMSCVhyg-3xT7-nSREBP1a. All plasmids were confirmed by DNA sequencing. Anti-MED1 (A300-793A) and anti-MED12 (A300-774A) were from Bethyl Laboratories. Anti-FASN (no. 3810), anti-SCD1 (no. 2794), and anti-T7 (no. 132469) were from Cell Signaling Technology. Anti-C/EBPα (sc-61X) and anti-PPARγ (sc-7196X) were from Santa Cruz Biotechnology. Anti-H3K27ac (ab4729), anti-GFP (ab290), anti-S5P Pol II (ab5131), and anti-S2P Pol II (ab5095) were from Abcam. Anti-β-Actin (A1978) and (Z)-4-hydroxytamoxifen (4OHT) (H7904) were from Sigma.

Jang Y., Park Y.K., Lee J.E., Wan D., Tran N., Gavrilova O, & Ge K. (2021). MED1 is a lipogenesis coactivator required for postnatal adipose expansion. Genes & Development, 35(9-10), 713-728.

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Antibody Characterization for Epigenetic Research

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The following antibodies were used in this study. Anti-C/EBPα (sc-61X, lot: G1112), anti-C/EBPβ (sc-150X, lot: L2910), anti-PPARγ (sc-7196X, lot: H1512), anti-p300 (sc-585X, lot: E2209) were from Santa Cruz Biotechnology (Dallas, TX, USA). Anti-CBP (7389S, lot: 1) was from Cell Signaling Technology (Danvers, MA, USA). Anti-MED1/TRAP220 (A300-793A, lot: 2), anti-RbBP5 (AA300-109A), anti-Menin (A300-105A) were from Bethyl Laboratories (Montgomery, TX, USA). Anti-EBF2 (AF7006, lot: CEUA0113081) was from R&D Systems (Minneapolis, MN, USA). Anti-CTCF (07-729, lot: 2054523), Anti-Pol II (17-672, lot: 2015592), anti-H3K4me3 (07-473, lot: 1974075) and Anti-H3K27me3 (07-449, lot: DAM1387952) were from Millipore (Billerica, MA, USA). Anti-H3K4me1 (ab8895, lot: GR61280-1), anti-H3K4me2 (ab7766, lot: GR102810-1), anti-H3K9me2 (ab1220, lot: 957002), anti-H3K27ac (ab4729, lot: GR81163-1), anti-H3K36me3 (ab9050, lot: 947436) were from Abcam (Cambridge, MA, USA). Anti-UTX was described previously (21 (link)). Anti-MLL4 and anti-SET1A were described previously (22 ).

Lai B., Lee J.E., Jang Y., Wang L., Peng W, & Ge K. (2017). MLL3/MLL4 are required for CBP/p300 binding on enhancers and super-enhancer formation in brown adipogenesis. Nucleic Acids Research, 45(11), 6388-6403.

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Generating Retroviral Vector with 3xT7-CA-ChREBP

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Triple T7 (3xT7) double StrepII tag was N-terminally subcloned into retroviral vector pMSCVhyg to generate pMSCVhyg-3xT7 using pTAG1-hygroTK-N-term 3xT7 double StrepII tag (gift from Rob Klose) 37 (link) as a template. Constitutive active form of mouse ChREBP was subcloned into pMSCVhyg-3xT7 to generate pMSCVhyg-3xT7-CA-ChREBP using full length clone as templates (Addgene #39235). All plasmids were confirmed by DNA sequencing. Anti-MED1 (A300-793A) and anti-MED12 (A300-774A) were from Bethyl Laboratories. Anti-FASN (#3810), anti-SCD1(#2794) and anti-T7 (#132469) were from Cell Signaling Technology. Anti-C/EBPα (sc-61X) and anti-PPARγ (sc-7196X) were from Santa Cruz Biotechnology. Anti-H3K27ac (ab4729), anti-GFP (ab290), anti-S5P Pol II (ab5131) and anti-S2P Pol II (ab5095) were from Abcam. Anti-β-Actin (A1978) and (Z)-4-hydroxytamoxifen (4OHT) (H7904) were from Sigma.

Jang Y., Park Y., Lee J., Tran N., Gavrilova O., & Ge K. (2020). MED1 is a lipogenesis coactivator required for postnatal adipose expansion.

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