Cells were harvested after treatments and RNA was isolated using TRIzol (Takara). cDNA for quantitative polymerase chain reaction (qPCR) assays was reversed transcribed from mRNA using a reverse transcription kit (AG11711) according to supplier’s protocols (Accurate Biotechnology Co.). All qPCR assays were conducted on a StepOnePlus device (Applied Biosystem) using a SYBR qPCR kit (AG11701, Accurate Biotechnology Co.) and the following primer pairs: antithrombin, 5′-GCTAAACCCCAACAGGGTGA-3′ (forward) and 5′-ACAAGGGTTGGCTACTCTGC-3′ (reverse); USP2,5′-GGGCTCCATAACGAGGTGAAC-3′ (forward) and 5′-CTCCACATCTGTCGGCCTTTC-3′ (reverse); and 18S RNA, 5′-AAACGGCTACCACATCCAAG-3′ (forward) and 5′-CCTCCAATGGATCCTCGTTA-3′ (reverse).
Sybr qpcr kit
Manufactured by Accurate Biology
The SYBR qPCR kit is a reagent system designed for quantitative real-time PCR (qPCR) analysis. It contains all the necessary components, including a DNA polymerase, SYBR Green dye, and buffers, to perform qPCR reactions. The SYBR Green dye binds to double-stranded DNA, allowing for the detection and quantification of target DNA sequences during the amplification process.
Automatically generated - may contain errors
3 protocols using sybr qpcr kit
1
Quantitative Analysis of Antithrombin and USP2
2
miR-203a-3p and MYD88 Expression Analysis
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Total RNA was extracted from rat primary chondrocytes using an RNAiso Plus Kit. Reverse transcription was performed with the PrimeScript RT kit. Both kits were purchased from Takara Biotechnology, Japan. The qPCR analyses were performed with the SYBR® qPCR Kit (Accurate Biotechnology) The downstream primers of miR-203a-3p were constructed using a universal downstream primer kit. The reaction conditions were set using a two-step PCR reaction program. The mRNA expression of miR-203a-3p and MYD88 were assessed using the 2−ΔΔCt method. The primer sequences were shown in Table 1 . The methods of Zhen Li et al. 2022 were followed [19 (link)].
3
Quantitative Analysis of Antithrombin and USP2
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were harvested after treatments and RNA was isolated using TRIzol (Takara). cDNA for quantitative polymerase chain reaction (qPCR) assays was reversed transcribed from mRNA using a reverse transcription kit (AG11711) according to supplier’s protocols (Accurate Biotechnology Co.). All qPCR assays were conducted on a StepOnePlus device (Applied Biosystem) using a SYBR qPCR kit (AG11701, Accurate Biotechnology Co.) and the following primer pairs: antithrombin, 5′-GCTAAACCCCAACAGGGTGA-3′ (forward) and 5′-ACAAGGGTTGGCTACTCTGC-3′ (reverse); USP2,5′-GGGCTCCATAACGAGGTGAAC-3′ (forward) and 5′-CTCCACATCTGTCGGCCTTTC-3′ (reverse); and 18S RNA, 5′-AAACGGCTACCACATCCAAG-3′ (forward) and 5′-CCTCCAATGGATCCTCGTTA-3′ (reverse).
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