Single cells were re-suspended in 0.1% fatty acid-free BSA-HBSS with 100 μM CaCl2 and 1 mM MnCl2 unless otherwise specified. Buffer without divalent cations included 10 mM EDTA. The cells were stained with fluorochrome-conjugated antibodies against various cell surface markers or with different fluorochrome-conjugated proteins, which are listed in the resource and reagent tables in the supplement. LIVE/DEAD Fixable Aqua Dead Cell Stain Kit, LIVE/DEAD Fixable NearIR Dead Cell Stain Kit, or LIVE/DEAD Fixable Yellow Dead Cell Stain Kit (Thermo Fisher) were used in all experiments to exclude dead cells. Compensation was performed using AbC Total Antibody Compensation Bead Kit (Thermo Fisher) and ArCTM Amine Reactive Compensation Bead (Thermo Fisher) individually stained with each fluorochrome. Compensation matrices were calculated with FACSdiva software. Data acquisition including cell number count was done on a FACSCelesta SORP (BD) flow cytometer and analyzed with FlowJo 10.8.x software (Tree Star).
Arctm amine reactive compensation bead
Manufactured by Thermo Fisher Scientific
The ArC™ Amine Reactive Compensation Bead is a laboratory reagent designed for compensation in flow cytometry applications. The beads are coated with amine-reactive dyes, allowing them to be used for setting compensation controls when analyzing samples with fluorescently-labeled antibodies or other reagents.
Automatically generated - may contain errors
Lab products found in correlation
Live dead fixable near ir dead cell stain kit, by Thermo Fisher Scientific (2 mentions)
Live dead fixable yellow dead cell stain kit, by Thermo Fisher Scientific (2 mentions)
Abc total antibody compensation bead kit, by Thermo Fisher Scientific (2 mentions)
Facs celesta sorp flow cytometer, by BD (2 mentions)
Live dead fixable aqua dead cell stain kit, by Thermo Fisher Scientific (2 mentions)
2 protocols using arctm amine reactive compensation bead
1
Single-Cell Surface Marker Staining
2
Multiparametric Flow Cytometry
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Single cells were re-suspended in 0.1% fatty acid free BSA-Hanks’ Balanced Salt Solution (HBSS) with 100 μM CaCl2 and 1 mM MnCl2 unless otherwise specified. Buffer without divalent cations included 10 mM EDTA. The cells were stained with fluorochrome-conjugated antibodies against various cell surface markers or with different fluorochrome-conjugated proteins, which are listed in the resource and reagent tables in the supplement . LIVE/DEAD™ Fixable Aqua Dead Cell Stain Kit, LIVE/DEAD™ Fixable NearIR Dead Cell Stain Kit or LIVE/DEAD™ Fixable Yellow Dead Cell Stain Kit (Thermo Fisher) were used in all experiments to exclude dead cells. Compensation was performed using AbC™ Total Antibody Compensation Bead Kit (Thermo Fisher) and ArCTM Amine Reactive Compensation Bead (Thermo Fisher) individually stained with each fluorochrome. Compensation matrices were calculated with FACSdiva software. Data acquisition included cell number count was done on a FACSCelesta SORP (BD) flow cytometer and analyzed with FlowJo 10.8.x software (Treestar).
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