Ham s f 12k

High performance liquid chromatography- (HPLC-) grade acetonitrile, HPLC-grade methanol, formic acid, sodium selenite, insulin, heparin, transferrin, ethylenediaminetetraacetic acid (EDTA), triphenylphosphine (TPP), sodium selenite, soybean lipoxidase type V, and linoleic acid were purchased from Sigma–Aldrich (St. Louis, MO). Diethyl ether and butylated hydroxy toluene (BHT) were purchased from ACROS Organics (Fair Lawn, NJ). YOPRO-1 and propidium iodide stains were from Thermo Fisher Scientific (Waltham, MA). Antibiotics/antimycotics, trypsin-EDTA, glutamine, and bovine collagen were from Life Technologies (Carlsbad, CA). All predesigned bovine TaqMan® primers were purchased from Applied Biosystems (Foster City, CA). Deuterated oxylipid standards, nondeuterated oxylipid standards, and indomethacin were purchased from Cayman Chemical (Ann Arbor, MI). Magnesium sulfate was purchased from Avantor Performance Materials, Inc. (Central Valley, PA), and sodium borate from Fisher Science Education (Nazareth, PA). Fetal bovine serum was purchased from Hyclone Laboratories, Inc. (Logan, Utah). The HEPES buffer, HAM's F-12k, and RPMI 1640 were from Corning Inc. (Corning, NY).

Fetal bovine serum was provided by Hyclone Laboratories, Inc. (Logan, UT). HAM's F-12K and HEPES buffer were from Corning Inc. (Corning, NY). Sodium selenite, insulin, heparin, and transferrin were from Sigma-Aldrich (St. Louis, MO). Antibiotics/antimycotics, glutamine, trypsin-EDTA, and bovine collagen were purchased from Life Technologies (Carlsbad, CA). Indomethacin, 15-F_2t-IsoP (formerly 8-isoPGF_2α), and 2,2'-azobis (2-amidinopropane) dihydrochloride (AAPH) were purchased from Cayman Chemical (Ann Arbor, MI). Lipopolysaccharide (LPS) purified from E. coli 0111:B4 was purchased from Invivogen (San Diego, CA).

HEK293FT (Invitrogen) cells were maintained in Dulbecco’s modified Eagle medium (DMEM, Corning) supplemented with 10% FBS (Corning), 1% Penicillin–Streptomycin-l-Glutamine (Corning), and 1% MEM Non-Essential Amino Acids (Gibco). CHO-K1 cells (ATCC) were maintained in Ham’s F-12K (Kaighn’s) medium supplemented with 10% FBS (Corning), 1% Penicillin–Streptomycin-l-Glutamine (Corning), and 1% MEM Non-Essential Amino Acids (Gibco). Doxycycline Hyclate (Dox, Sigma-Aldrich) was diluted in water at 10 mg/μL, stored at −20 ^∘C, and used at a final concentration of 4 μM for experiments. Trichostatin A (TSA, Sigma-Aldrich) was diluted in DMSO, stored at −20 ^∘C, and used at a final concentration of 100 nM for experiments.