Gastric cancer cell lines (AGS and MGC80-3) were purchased from Nanjing KeyGen Biotech Co., Ltd. RPMI 1640 medium (Abcam) containing 10% FBS (Abcam) and 100 U/ml penicillin and 100 µg/ml streptomycin (Invitrogen; Thermo Fisher Scientific, Inc.) was used to cultivate gastric cancer cell lines at 37°C with 5% CO2.
Rpmi 1640 medium
Manufactured by Abcam
RPMI 1640 medium is a commonly used cell culture medium. It provides a balanced salt solution and essential nutrients to support the growth and maintenance of a variety of cell types in vitro.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin, by Thermo Fisher Scientific (1 mentions)
Streptomycin, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Abcam (1 mentions)
Mgc803, by Keygen Biotech (1 mentions)
Sgc 7901, by Genechem (1 mentions)
Mkn 28, by Genechem (1 mentions)
Mgc 803, by Genechem (1 mentions)
Epidermal growth factor (egf), by Abcam (1 mentions)
Mkn 45, by Genechem (1 mentions)
Ges 1, by Genechem (1 mentions)
Rpmi 1640 medium, by Corning (1 mentions)
2 protocols using rpmi 1640 medium
1
Gastric Cancer Cell Culture Protocol
2
EGF-Induced HCCR-1 Expression in GC Cells
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Six GC cell lines (AGS, SGC-7901, MKN-45, MKN-28, MGC-803, and BGC-83) and one normal gastric mucosa cell line (GES-1) were purchased from GeneChem (Shanghai, China) and cultured in RPMI-1640 medium (Corning, USA). The SGC-7901 cells were cultured in serum-free RPMI-1640 medium for 24 hours, followed by culturing in different EGF (Abcam) concentrations (0, 60, and 120 ng/mL) for 24 hours and culturing in 60 ng/mL of EGF for different periods (0, 12, and 24 hours). Changes in the expression of HCCR-1 were then detected in the cells by WB.
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