SUVs were prepared by rehydrating a lipid film composed primarily of POPC (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine, Avanti Polar Lipids), doped with 0.5% of Biotinyl Cap PE (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(cap biotinyl), Avanti Polar Lipids), 1% DGS-Ni-NTA (1,2-dioleoyl-sn-glycero-3-[(N-(5-amino1-carboxypentyl)iminodiacetic acid)succinyl] with nickel salt, Avanti Polar Lipids), and 0.4% LISS-Rhodamine (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl), Avanti Polar Lipids) in pure deionized H2O. The rehydrated solution was vortexed briefly and then sonicated using a tip-sonicator at 20% power pulsing off and on for 3 minutes. SUVs were then filtered through a 200 nm PTFE filter (Millipore). SUV solutions were stored at 4°C and used within 48 hours of preparation to avoid phospholipid oxidization.
200 nm ptfe filter
Manufactured by Merck Group
The 200 nm PTFE filter is a laboratory filtration device designed to remove particles and contaminants from liquids or gases. It is constructed with a polytetrafluoroethylene (PTFE) membrane that has a nominal pore size of 200 nanometers, allowing it to effectively filter out small particulates while maintaining high flow rates. The filter can be used in a variety of laboratory applications where precise filtration is required.
Automatically generated - may contain errors
2 protocols using 200 nm ptfe filter
1
Preparation of Biotinylated and Fluorescent SUVs
2
Preparation of Biotinylated and Fluorescent SUVs
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SUVs were prepared by rehydrating a lipid film composed primarily of POPC (1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine, Avanti Polar Lipids), doped with 0.5% of Biotinyl Cap PE (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(cap biotinyl), Avanti Polar Lipids), 1% DGS-Ni-NTA (1,2-dioleoyl-sn-glycero-3-[(N-(5-amino1-carboxypentyl)iminodiacetic acid)succinyl] with nickel salt, Avanti Polar Lipids), and 0.4% LISS-Rhodamine (1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(lissamine rhodamine B sulfonyl), Avanti Polar Lipids) in pure deionized H2O. The rehydrated solution was vortexed briefly and then sonicated using a tip-sonicator at 20% power pulsing off and on for 3 minutes. SUVs were then filtered through a 200 nm PTFE filter (Millipore). SUV solutions were stored at 4°C and used within 48 hours of preparation to avoid phospholipid oxidization.
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