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Anhydrous acn

Manufactured by Merck Group

Anhydrous ACN is a laboratory reagent used as a solvent and chemical intermediate. It is a clear, volatile liquid with a characteristic odor. Anhydrous ACN has a high boiling point and is miscible with a wide range of organic solvents. It is commonly used in various chemical synthesis and analytical applications.

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3 protocols using anhydrous acn

1

Fabrication of Composite Solid Electrolytes

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PEO (Mw: 3×105 g mol−1, Sigma‐Aldrich) and LiTFSI (Sigma‐Aldrich) was dried under vacuum at 60 and 100 °C for 24 h prior to use. Anhydrous ACN (Sigma‐Aldrich) was used as received. LLZO with the composition of Li6.4La3Zr1.4Ta0.6O12 were synthesized by solid‐state reaction,[49
] and then high‐energy ball‐milled. PEO/LiTFSI and cast‐CSE were prepared by tape casting the PEO/LiTFSI/ACN solution and LLZO/PEO/LiTFSI/ACN solution with EO/Li ratio of 8 and then dried in a vacuum oven at 50 °C for 48 h. ES‐CSE (or AES‐CSE) was prepared by electrospinning with the LLZO/PEO/LiTFSI/ACN as core solution and PEO/LiTFSI/ACN as shell solution under 17 kV using a drum collector rotating at 60 rpm (or 800 rpm) and then dried in a vacuum oven at 50 °C for 48 h. The LLZO contents in the core solutions were 15 to 48 wt%.
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2

TMT Labeling for Quantitative Proteomics

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TMT‐10plex (Thermo Fisher: 90110) labels (0.8 mg) were resuspended in 100 μl anhydrous ACN (Sigma: 271004), vortexed and incubated for 5 min at room temperature. Freeze‐dried eluted peptides were resuspended in 80 μl 100 mM TEAB (Thermo Fisher: 90114) and incubated in an ultrasonic bath for 15 min. Seventy microlitres of each sample was combined with 30 μl of the respective TMT10plex label (see Table EV2). TMT reaction was carried out at room temperature for 2 h. An aliquot of 10 μl of each sample was taken, and the reaction was stopped by addition of 50% (w/v) hydroxylamine (Sigma: 467804) to a final concentration of 5% (v/v). Stopped TMT reactions were combined and freeze‐dried overnight.
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3

Cocaine and Metabolite Analysis Protocol

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Stock solutions at 1 mg mL -1 of BZE in MeOH and cocaine base were provided by Sigma-Aldrich (Saint-Quentin-Fallavier, France). The working solutions at 1 µg mL -1 were obtained by dilution of the stock solution (1 mg mL -1 ) in HPLC-grade ACN (Carlo Erba, Val-de-Reuil, France). These solutions were stored at -20°C. MeOH, EtOH, and isooctane were purchased from Carlo Erba. Acetic acid and anhydrous ACN were provided by Sigma-Aldrich. 1 M NaOH and 0.1 M HCl were obtained using deionized water (Milli-Q purification system, Millipore, Molsheim, France) and Titrisol solutions from Merck (Darmstadt, Germany). MAA, TRIM, and 3-(trimethoxysilyl)propyl methacrylate (γ-MAPS) were purchased from Sigma-Aldrich. Azo-N,N'-diisobutyronitrile (AIBN) was purchased from Acros Organics (Noisy-le-Grand, France). MAA was distilled under vacuum in order to remove inhibitors and stored at -20°C. TRIM, AIBN, and γ-MAPS were of satisfactory purity so they were used without further purification. Fused-silica capillaries with a UV transparent fluorocarbon polymer coating (PTFE-coated silica capillaries, 100 µm i.d. x 375 µm outer diameter (o.d.)) were purchased from Polymicro Technologies (Photon Lines, Saint Germain-en-Laye, France).
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