Rnaiso plus

Total RNA from HSC-T6 cells was extracted using RNAiso Plus (Transgen Biotech, Beijing, China) following the manufacturer’s protocol, and the purity of the extracted RNA was determined. The primers of COL3A1 [collagen type III alpha 1 chain], COL1A1 (collagen type I alpha 1 chain), α-SMA (α-smooth muscle actin), SDC-4 (syndecan-4), and GAPDH are listed in Table 1. cDNA was synthesized using a PrimeScript^® RT reagent kit according to the manufacturer’s instructions (Transgen Biotech, Beijing, China). For real-time PCR assay, SYBR^® Premix Ex Taq^TM II (Transgen Biotech, Beijing, China) was used and subjected to quantitative PCR in an ABI 7500 Real Time PCR System, and the data was analyzed using System SDS software (Applied Biosystems, United States).

Total RNA was extracted from the lily petals at FS using RNAiso Plus (TransGen Biotech, Beijing, China). The quality and quantity of purified RNA were examined using a NanoDrop ND-1000 UV/Visible spectrophotometer (Wilmington, DE, USA). The RIN (RNA integrity number) values (>8.0) of these samples were assessed using an Agilent 2100 Bioanalyzer (Agilent Technologies, Santa Clara, CA, USA) for gel electrophoresis. High-quality RNA was used in cDNA library construction and Illumina deep sequencing.