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Cd133 1 ac133 pe antibody

Manufactured by Miltenyi Biotec
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The CD133/1 (AC133) - PE antibody is a laboratory reagent used for the detection and analysis of CD133-positive cells. CD133 is a surface antigen that is expressed on various stem and progenitor cell populations. The PE (Phycoerythrin) fluorescent label allows for the identification and quantification of CD133-positive cells using flow cytometry or other analytical techniques.

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CD133 (122 citations) CD133-PE (52 citations) AC133 (16 citations) CD133 antibody (15 citations) CD133-PE antibody (14 citations) CD133/1-PE (10 citations) CD133/1(AC133)-PE (6 citations) PE-conjugated human CD133/1 antibody (clone AC133, (5 citations) Anti-CD133/1 (AC133)-PE antibody (3 citations) PE)-conjugated CD133/1 clone AC133 antibody (2 citations)

2 protocols using cd133 1 ac133 pe antibody

1

CD133-Based Cell Sorting Protocol

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Cells were labeled with CD133/1 (AC133) - PE antibody (Miltenyi Biotec), and high and low CD133 expressing cells were subjected to sterile sorting by flow cytometry.
Liu Q., Wang M., Kern A.M., Khaled S., Han J., Yeap B.Y., Hong T.S., Settleman J., Benes C.H., Held K.D., Efstathiou J.A, & Willers H. (2015). Adapting a Drug Screening Platform to Discover Associations of Molecular Targeted Radiosensitizers with Genomic Biomarkers. Molecular cancer research : MCR, 13(4), 713-720.
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2

CD133 Expression Sorting Protocol

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Cells were incubated with CD133/1 (AC133) - PE antibody (Miltenyi Biotec, Cambridge, MA) with the dilution of 1:11 for up to 107 cells/100 μL of buffer at 4 °C for 2 hours. High and low CD133 expressing cells were subjected to sterile sorting by flow cytometry with 4 Laser BD FACSAria II Cell Sorter at MGH Pathology Flow Cytometry Core.
Wang M., Han J., Marcar L., Black J., Liu Q., Li X., Nagulapalli K., Sequist L.V., Mak R.H., Benes C.H., Hong T.S., Gurtner K., Krause M., Baumann M., Kang J.X., Whetstine J.R, & Willers H. (2017). Radiation resistance in KRAS-mutated lung cancer is enabled by stem-like properties mediated by an osteopontin-EGFR pathway. Cancer research, 77(8), 2018-2028.
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