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Mouse hematopoietic lineage biotin panel

Manufactured by Thermo Fisher Scientific

The Mouse Hematopoietic Lineage Biotin Panel is a laboratory equipment product designed for the identification and analysis of mouse hematopoietic lineage cells. It provides a comprehensive set of antibodies labeled with biotin, allowing for the detection and characterization of various cell populations in mouse samples.

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2 protocols using mouse hematopoietic lineage biotin panel

1

Multiparametric Immunophenotyping of Hematopoietic Cells

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Anti- mouse Ter119-BV421, Sca-1-APC, CD34-FITC, CD135-PE, IL-7Ra-BV605, CD48-FITC, CD150-PE Cy7, CD45.2-Alexa Fluor 700/APC Cy7, CD11b-BV605, CD3-APC, NK1.1-APC Cy7, CD19-BV605, IgM-Alexa Fluor 647, CD43-PE Cy7, CD16/32-PerCP Cy5.5, CD16/32; anti-human CD34-APC Cy7 (clone: 581), CD38-PE Cy7 (HB-7), CD45RA-BV 605 (HI100), CD90-APC (5E10), CD49f-PerCP Cy5.5 (GoH3), CD45-PE/Dazzle 594 (HI30), and CD33-APC (P67.6) antibodies; and Legendplex™ Mouse Inflammation Panel (13-plex) and Mouse IL-6 ELISA were purchased from Biolegend (San Diego, CA). IL6 depleting AB (clone MP5–20F3) from BioXCell (West Lebanon, NH).
Anti-mouse c-Kit-PE Cy7, Mouse Hematopoietic Lineage Biotin Panel, and anti-human CD19-Alexa Fluor 700 (HIB19) from eBioscience (San Diego, CA). Anti-B220-PE CF594 from BD biosciences (San Jose, CA). EasySep Human Progenitor Cell Enrichment Kit from Stem Cell Technologies Inc. (Vancouver, BC). Anti-human EPCR-FITC (RCR-49) from Santa Cruz Biotechnology®, inc. (Dallas, TX). Anti-human CD141-PE (AD5–14H12) from Miltenyi Biotec Inc. (Auburn, CA).
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2

Radiation-Induced Hematopoietic Changes

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Mice were irradiated with 3 Gy for reasons describes earlier; DAS was administered 2 hours after irradiation. Twenty-four hours post treatment bone marrow cells were taken out from mice of each treated and untreated groups. Cells were washed twice with PBS and then labeled with antibodies against specific surface markers like CD3 for T-cells, B220 for B-cells, Cb11b for monocytes, TER for erythrocytes, and Ly-6G for lymphocytes and analyzed using flow cytometer following the manufacturer's instructions (EBioscience Mouse Hematopoietic lineage Biotin Panel and catalog number 88-7774).
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