Cp 3800 saturn 2000 apparatus

HS-SPME analysis (30 min exposure to a 2 cm DVB/CAR/PDMS fiber, Supelco, Bellefonte, PA, USA, followed by analyte desorption at 220 °C for 3 min) was performed on Varian CP-3800/Saturn 2000 apparatus (Varian, Walnut Creek, CA, USA) equipped with a Zebron ZB-5 MSI (30 m × 0.25 mm × 0.25 µm) column (Phenomenex, Shim-Pol, Poland). About 0.100 g of fresh or 0.150 g of dried sample was put in to headspace vials and kept in laboratory water bath at 70 °C. 0.5 mg of 2-undecanone (Sigma Aldrich, Saint Louis, MO, USA) as an internal standard was added.

Nuclear magnetic resonance (NMR) spectra were recorded in CDCl₃ or in C₆D₆ solutions on a Bruker Avance II 300 MHz Bruker spectrometer (Bruker, Rheinstetten, Germany) with signals of residual solvents as references for chemical shifts.
IR spectra were recorded for liquid films on a Mattson IR 300 Thermo-Nicolet spectrophotometer (Waltham, MA, USA).
EI-MS spectrum: Gas chromatography of isolated compounds was performed on Varian CP-3800/Saturn 2000 apparatus (Varian, Walnut Creek, CA, USA) equipped with a Zebron ZB-5 MSI (30 m × 0.25 mm × 0.25 µm) column (Phenomenex, Shim-Pol, Poland). The GC oven temperature was programmed from 100 °C to 200 °C at rate 10.0 °C, then to 290 °C at rate 15.0 °C, and held for 5 min. Scanning was performed from 35 to 550 m/z in electronic impact (EI) mode at 70 eV. Samples were injected at a 1:10 split ratio, and helium gas was used as the carrier gas at a flow rate of 1.0 mL·min⁻¹. Spectra were recorded in electron impact mode.

HS-SPME analysis was applied with 2 cm DVB/CAR/PDMS fibre (Supeclo, Bellefonte, PA, USA). About 0.100 g of fresh flowers or 0.150 g of dried flowers were put into a headspace glass vial and 0.5 mg of 2-undecanone (Sigma-Aldrich, Saint Louis, MO, USA) as an internal standard was added. Then, the vial was placed in a laboratory water bath at 70 °C, and the fibre was exposed above the sample (headspace) for 30 min and followed by analyte desorption in GC injector for 3 min at 220 °C. A GC-MS analysis was performed on Varian CP-3800/Saturn 2000 apparatus (Varian, Walnut Creek, CA, USA) equipped with Zebron ZB-5 MSI (30 m × 0.25 mm × 0.25 µm) column (Phenomenex, Torrance, CA, USA). The GC oven temperature was programmed from 50°C to 130 °C at a rate of 4.0 °C, then to 180 °C at a rate of 10.0 °C, then to 280 °C at a rate of 20.0 °C. Scanning was performed from 35 to 550 m/z in electronic impact (EI) mode at 70 eV. Samples were injected at a 1:10 split ratio, and helium gas was used as the carrier gas at a flow rate of 1.0 mL·min⁻¹. Analyses were run in triplicate.