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2 protocols using 1 m hydrochloric acid

1

Electrochemical Detection of Biomolecules

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Cortisol (C21H30O5, hydrocortisone), β-CD [(C6H10O5)7], potassium HCF(II)
trihydrate (K4[Fe(CN)6]·3H2O),
potassium HCF(III) (K4[Fe(CN)6]), graphite powder,
sulfuric acid (H2SO4, 98%), sodium nitrate (NaNO3), potassium permanganate (KMnO4), hydrogen peroxide
(H2O2, 30% in water), 1 M hydrochloric acid
(HCl), ethanol, dimethyl sulfoxide (DMSO), glucose, urea, sodium l-lactate, cholesterol, progesterone, testosterone, sodium chloride
(NaCl), disodium hydrogen phosphate (Na2HPO4), anhydrous calcium chloride (CaCl2), and mucin were
procured from Fujifilm WAKO (Japan). Phosphate buffer saline (PBS,
pH 7.4) and ultrapure water were obtained from Gibco (Thermofischer).
Pyrrole (C4H5N) was obtained from Tokyo Chemicals
(TCI, Japan). All chemicals were used as received. Rod-type glassy
carbon electrodes (GCE, surface area = 0.07 cm2) were obtained
from ALS Co., Ltd. (Japan).
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2

Quantitative Analysis of Risedronate

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Chemicals Risedronate and 1-octyltriethylammonium phosphate as an ion-pairing (IP) reagent were obtained from LKT Laboratories (St. Paul, MN, U.S.A.). Ammonium dihydrogen phosphate, calcium chloride, 1 M hydrochloric acid, and 1 M sodium chloride were obtained from Wako Pure Chemical Industries, Ltd. (Osaka, Japan). Ethylenediamine-N,N,N′,N′-tetraacetic acid, disodium salt (EDTA/2 Na), as a chelating agent, and tetrabutylammonium bromide were obtained from Sigma-Aldrich (St. Louis, MO, U.S.A.). Methanol was obtained from Kanto Chemical Industries (Tokyo, Japan). 2-Pyridylacetic acid hydrochloride, as an internal standard (IS), etidronate (ca. 60% in water, ca. 4.2 mol/L), and ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid (EGTA), used as a calcium scavenger, were obtained from Tokyo Chemical Industry (Tokyo, Japan). OASIS ® HLB cartridges (Waters Corporation, Milford, MA, U.S.A.) were used for solid-phase extraction. Ultrapure water was generated using a Millipore Milli-Q Plus system (Merck Millipore, Billerica, MA, U.S.A.).
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