Genomic DNA was isolated from cell cultures using QIAGEN DNeasy protocol (QIAGEN, Hilden, Germany). PCR was performed using TaqMan Fast Master Mix (Invitrogen, Carlsbad, CA, USA) and 0.9 μM GAG forward (5′-GGAGCTAGAACGATTCGCAGTTA-3′) and reverse (5′-GGTTGTAGCTGTCCCAGTATTTGTC-3′) primers and GAG FAM probe [5′-(FAM)-ACAGCCTTCTGATGTCTCTAAAAGGCCAGG-(TAMRA)-3′] and RNASE-P-VIC control TaqMan assay (Invitrogen), and run using Fast program on Applied Biosystems StepOnePlus real-time thermocycler (Invitrogen). VCN was assessed relative to the Clone K3 cDNA, known to contain two copies of integrated viral DNA per cell.2 (link)
Rnase p vic control taqman assay
Manufactured by Thermo Fisher Scientific
Sourced in United States
The RNASE-P-VIC control TaqMan assay is a molecular biology tool designed for the detection and quantification of the RNase P gene, which serves as an internal control for real-time PCR experiments. The assay utilizes the TaqMan technology, which employs a fluorescent probe to monitor the amplification of the target sequence during the PCR reaction. The VIC dye is used to label the probe, providing a specific signal for the RNase P gene.
Automatically generated - may contain errors
2 protocols using rnase p vic control taqman assay
1
Genomic DNA Extraction and Viral Copy Number Quantification
2
Quantification of Lentiviral Vector Copy Number
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Genomic DNA was extracted using the QIAGEN DNeasy protocol (QIAGEN, Hilden, Germany). VCN was assessed by real-time polymerase chain reaction, performed using TaqMan Fast Master Mix (Invitrogen, Carlsbad, CA, USA) and 0.9 mM GAG forward (5′-GGAGCTAGAACGATTCGCAGTTA-3′) and reverse (5′-GGTTGTAGCTGTCCCAGTATTTGTC-3′) primers, GAG FAM probe (5′-[FAM]-ACAGCCTTCTGATGTCTCTAAAAGGCCAGG-[TAMRA]-3′), and RNASE-P-VIC control TaqMan assay (Invitrogen). PCR was run using Fast program on Applied Biosystems StepOnePlus real-time thermocycler (Invitrogen, Carlsbad, CA, USA). VCN was assessed relative to a reference known to contain one copy of integrated viral DNA per haploid genome.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!