The following primary antibodies were used: rabbit anti-TH (1:1000, Pel-Freez Biologicals, #P40101-150), sheep anti-TH (1:2000, Novus Biologicals, #NB300-110), rabbit anti-serotonin (1:2000, ImmunoStar, #20080), chicken anti-GFP (1:600, Aves Labs Inc., #GFP-1020), goat anti-human SOX10 (1:800, R&D Systems, #AF2864), rabbit anti-KI67 (1:500, Thermo Scientific, #RM-9106), rabbit anti-Cleaved Caspase-3 (1:500, Cell signaling, Asp175), rat anti-BrdU (1:300, Abcam, BU1/75, also recognizes CldU).
For detection of the primary antibodies, secondary antibodies raised in donkey and conjugated with Alexa-405, -488, -555, and -647 fluorophores were used (1:1000, Molecular Probes, ThermoFisher Scientific). Goat anti-chicken conjugated to Alexa fluor-488 (1:600, Jackson ImmunoResearch, 703-545-155).
For detection of the primary antibodies, secondary antibodies raised in donkey and conjugated with Alexa-405, -488, -555, and -647 fluorophores were used (1:1000, Molecular Probes, ThermoFisher Scientific). Goat anti-chicken conjugated to Alexa fluor-488 (1:600, Jackson ImmunoResearch, 703-545-155).