Nicolet is50 ft ir infrared spectrometer

IR spectra (PLCL fibers, PLCL/heparin fibers, and heparin) were acquired using a Thermo Nicolet iS50 FTIR infrared spectrometer. Each spectrum was obtained by averaging 64 scans, and the wavenumber range spanned from 400 to 4,000 cm⁻¹.

¹H NMR spectra were measured by Bruker 600 MHz nuclear magnetic resonance spectrometer. Mass spectra were obtained by Thermo Q Exactive field orbital well cyclotron resonance mass spectrometer. IR spectra (solid infrared, liquid infrared and time-resolved infrared) were carried out by Thermo Nicolet iS50 FT-IR infrared spectrometer. UV-vis spectra were measured by a Thermo Evolution-220 spectrometer. Elemental analysis was measured by a vario EL CUBE. XRD was conducted by a Bruker Smart Apex II diffractometer. EPR was measured by a Bruker EMXPLUS10/12 spectrometer. Fluorescence spectra were measured by a FluoroMax-4 spectrofluorometer. The molecular weight of protein was analyzed by Bruker Ultraflex MALDI-TOF-MS spectrometer. Agarose gel electrophoresis was performed by DYY-6C electrophoresis apparatus of Beijing Liuyi Biological Technology Co., Ltd (Beijing, China). A cytotoxicity test was measured by SpectraMax iD5 microplate reader. Confocal microscopy images were analyzed by a LSM-880 confocal laser scanning microscope.