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Rabbit anti human sma

Manufactured by Abcam

Rabbit anti-human SMA is a primary antibody that specifically recognizes human smooth muscle actin (SMA). It is designed for use in immunohistochemistry, immunofluorescence, and Western blotting applications to identify and quantify SMA-expressing cells.

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2 protocols using rabbit anti human sma

1

Western Blot Analysis of EMT Markers

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Western blots were performed using an SDS–PAGE electrophoresis system as described previously [19 (link)], employing rabbit anti-human EREG (Abcam), rabbit anti-human SMA (Abcam), rabbit anti-human E-cadherin (CST), rabbit anti-human N-cadherin (CST), rabbit anti-human vimentin (Proteintech), rabbit anti-human p-JAK2 (CST), rabbit anti-human JAK2 (CST), rabbit anti-human p-STAT3 (CST), mouse anti-human STAT3 (CST), rabbit anti-human p-NF-kB (CST), rabbit anti-human NF-kB (CST), rabbit anti-human p-p38 (CST), rabbit anti-human p38 (CST), rabbit anti-human p-akt (CST), rabbit anti-human akt (CST), rabbit anti-human p-erk1/2 (CST), rabbit anti-human erk1/2 (CST), rabbit anti-human snail (CST), antibodies. The blots were re-probed for rabbit anti-human GAPDH (Bioworld) to control for protein loading and transfer.
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2

Immunofluorescence Analysis of EREG, SMA, and CK

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Immunofluorescence analyses were carried out as previously described [20 (link)]: primary antibodies, goat anti-human EREG (R&D), rabbit anti-human SMA (Abcam) and mouse anti-human CK (Abcam); secondary antibodies, AlexaFluor 647 donkey anti-goat IgG and AlexaFluor 488 goat anti-rabbit 594 goat anti-mouse IgG (Life Technologies).
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