Mab5643

The following primary antibodies were used: anti-APP CT (A8717, Sigma-Aldrich, St. Louis, MO); anti-β-amyloid, 17–24 (clone 4G8) (800701, BioLegend, San Diego, CA); anti-PS1 NT raised against the N terminus of PS1 (APS11) (ab15456, Abcam, Cambridge, MA); anti-PS1 CT raised against the C-terminus of PS1 (mAb5643, Cell Signaling Technology, Danvers, MA); anti-PS1 loop raised against the loop domain between transmembrane domains 6 and 7 of PS1 (E2000Y) (ab76083, Abcam, Cambridge, MA); anti-Syt1 (AB5600, Millipore, Temecula, CA); anti-Gapdh (mAb2118, Cell Signaling Technology, Danvers, MA); anti-MAP2 (ab5392, Abcam, Cambridge, MA); anti-His (ab18184, Abcam, Cambridge, MA); anti-V5 (ab9116, Abcam, Cambridge) and anti-FLAG M2 (F1804, Sigma-Aldrich, St. Louis, MO). Alexa Fluor 488 (ThermoScientific, Waltham, MA) and Cy3-labeled corresponding secondary antibodies (Jackson ImmunoResearch, West Grove, PA) were used for confocal microscopy imaging, and IRDye680/800- (Li-COR, Lincoln, NE) or HRP- (Jackson ImmunoResearch, West Grove, PA) conjugated ones were used for western blotting.

The following primary antibodies were used: anti-GLT-1 raised against the peptide within 30 C-terminal amino acids from the canonical GLT-1 sequence (AB1783, EMD Millipore, Temecula, CA), and anti-GLT-1 raised against the peptide corresponding to residues 550 to the C-terminus of rat GLT-1 (ab41621, Abcam, Cambridge, MA), both recognizing GLT-1a. The GLT-1 antibody from Abcam was used in the co-IP experiments and the antibody from EMD Millipore for the immunofluorescent staining. Anti-GLAST (MABN794, Millipore, Temecula, CA); anti-EAAT3 (MAB1587, Millipore, Temecula, CA); anti-Na⁺/K⁺-ATPase (05-369, Millipore, Temecula, CA); anti-PS1 loop raised against the loop domain between transmembrane domains 6 and 7 of PS1, clone APS18 (ab15458, Abcam, Cambridge, MA) and anti-PS1 CT raised against the C-terminus of PS1 (mAb5643, Cell Signaling Technology, Danvers, MA and P7854, Sigma-Aldrich, St. Louis, MO). Alexa Fluor 488 (ThermoScientific, Waltham, MA) and Cy3-conjugated secondary antibodies (Jackson ImmunoResearch, West Grove, PA) were applied for the microscopy imaging and IRDye680/800- (Li-COR, Lincoln, NE) labeled ones were used for western blotting.