The animals were euthanized after 21 days in the IC experiment and 30 days in both the SQ and IT experiments. Tumors, tissue specimens and bones were collected at necropsy. Tumor and tissue specimens were fixed in 10% neutral-buffered formalin at room temperature for 72 hr, embedded in paraffin, cut in 4 μm sections, and stained with hematoxylin and eosin (H&E). Bones were decalcified with Mild Decalcifier (formaldehyde, methanol and formic acid) (Leica Biosystems, Buffalo Grove, IL) at room temperature for 6 hr. Histological images of the slides were taken using an Olympus BX51 microscope equipped with a Nikon digital camera and analyzed using ImageScope software (version 11.2, Leica Biosystems, Buffalo Grove, IL).
Mild decalcifier
Manufactured by Leica
Sourced in United States
Mild Decalcifier is a laboratory solution designed to gently remove calcium deposits from samples without causing significant damage to the surrounding tissue. It is a key tool for preparing specimens for further analysis and examination.
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Lab products found in correlation
3 protocols using mild decalcifier
1
Histological Analysis of Tumor Samples
2
Metastatic Tumor Xenograft Analysis
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The mice bearing subcutaneous tumors were euthanized after 4 weeks of treatment with either SP600125 or vehicle. Organs and subcutaneous xenografts were collected and examined to confirm metastases and tumor growth. All xenografts were weighed and divided into 2 halves: 1 half was immediately snap-frozen in liquid nitrogen and kept at −80 °C, and the other half was fixed in 10% natural-buffered formalin for 48 h, embedded in paraffin, sectioned, and stained with hematoxylin and eosin (HE).
The mice bearing intratibial tumors were euthanized at day 15 after they were transplanted with the tumor cells. Tibial bones were decalcified in a mild decalcifier (formaldehyde, methanol, and formic acid) (Leica Biosystems Inc., Buffalo Grove, IL, USA, 60089) at 37 °C for 4 h before histopathological processing. Images were acquired using an Olympus BX51 microscope (Olympus America, Inc., Melville, NY, USA, 11747) equipped with a Nikon digital camera (Nikon Inc., Melville, NY, USA, 11747).
The mice bearing intratibial tumors were euthanized at day 15 after they were transplanted with the tumor cells. Tibial bones were decalcified in a mild decalcifier (formaldehyde, methanol, and formic acid) (Leica Biosystems Inc., Buffalo Grove, IL, USA, 60089) at 37 °C for 4 h before histopathological processing. Images were acquired using an Olympus BX51 microscope (Olympus America, Inc., Melville, NY, USA, 11747) equipped with a Nikon digital camera (Nikon Inc., Melville, NY, USA, 11747).
3
Histological Analysis of Tumor Necrosis
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