Sc 24973a

Manufactured by Santa Cruz Biotechnology

Sourced in United States

Sc-24973A is a laboratory equipment product offered by Santa Cruz Biotechnology. It is a technical device designed for use in scientific research and development applications. No further details are available at this time.

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Lab products found in correlation

Paraformaldehyde (pfa), by Santa Cruz Biotechnology (3 mentions) Dm500, by Leica (2 mentions) Sc 281692, by Santa Cruz Biotechnology (1 mentions) Rm2245, by Leica (1 mentions) Prism 6, by GraphPad (1 mentions) C1210, by Applygen (1 mentions) Hoechst 33342, by Merck Group (1 mentions)

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Hematoxylin (sc-24973A (2 citations)

5 protocols using sc 24973a

Morphological Analysis of Ovarian Follicles

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For morphological analysis, the ovaries were fixed in 4% paraformaldehyde (PFA, Santa Cruz, 30525-89-4) for 8 h at 4 °C, dehydrated in ethanol and xylene, embedded in paraffin, then sectioned serially at 8 μm, deparaffinized and rehydrated. To observe tissue morphology and count the follicle number, sections were stained with hematoxylin (Santa Cruz, sc-24973A). The primordial follicles and primary follicles were counted in every fifth section and multiplied by five to calculate the total number in each ovary. The growing follicles after primary follicles were counted by scanning every section, and only the follicles with clear oocyte nuclei were counted in the ovaries. The total number of follicles was summed by the number of primordial follicles and growing follicles.
To compare the ovarian development in C57 and C3H females, the secondary follicle ratio was counted in 5 dpp ovaries (7 females for each strain), and the diameters and GCs layers of growing follicles were determined by counting the five largest follicles [49 (link)] in the 13 dpp ovaries (8 females for each strain).

Dai Y., Bo Y., Wang P., Xu X., Singh M., Jia L., Zhang S., Niu S., Cheng K., Liang J., Mu L., Geng K., Xia G., Wang C., Zhang Y, & Zhang H. (2022). Asynchronous embryonic germ cell development leads to a heterogeneity of postnatal ovarian follicle activation and may influence the timing of puberty onset in mice. BMC Biology, 20, 109.

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Ovarian Follicle Quantification Protocol

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The ovaries were fixed in 4% (w/v) paraformaldehyde (SC-281692, Santa Cruz) for 12 to 24 hours and then embedded in paraffin (39601095, Leica) after dehydration. Then, the ovaries were serially cut into 8-μm sections with a microtome (RM2245, Leica), and all sections were carefully analyzed under the microscope (DM500, Leica). To count the follicle number, tissue sections were stained with hematoxylin (SC-24973A, Santa Cruz). PFs were counted in every third section and multiplied by three to calculate the number of all PFs in each ovary. Every section was counted for the presence of GFs. The number of PFs and GFs was summed to the total number of follicles.
We studied the effect of inhibiting the vascularization of adult ovaries on the activation rate of PFs by counting the ratio of transient (59 (link)) follicles. The transient follicles were identified by a mixed flatten and cuboidal GCs of the follicles. The percentage of activated PFs was quantified as the number of transient follicles divided by the total number of PFs in the ovary.

Xu X., Mu L., Li L., Liang J., Zhang S., Jia L., Yang X., Dai Y., Zhang J., Wang Y., Niu S., Xia G., Yang Y., Zhang Y., Cao Y, & Zhang H. (2022). Imaging and tracing the pattern of adult ovarian angiogenesis implies a strategy against female reproductive aging. Science Advances, 8(2), eabi8683.

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Quantitative Ovarian Follicle Analysis

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Ovaries were collected at specific ages and then fixed in 4% Paraformaldehyde solution in PBS (PFA, sc-281692, Santa Cruz Biotechnology, United States). The embedded ovaries were dehydrated and then sectioned to obtain 8-µm thickness serial paraffin sections. The ovarian sections were processed to deparaffinize and rehydrate, the ovarian sections were stained with hematoxylin (sc-24973A, Santa Cruz Biotechnology, United States) to analyze the histology of the ovaries. The images were all analyzed under the microscope (DM500, Leica). Every five slices were counted for the number of primordial and primary follicles under 100 μm. Then, the counting numbers were multiplied by five to calculate the number of all primordial or primary follicles. Each slice was counted for the number of secondary follicles, antral follicles and the structure of the corpus luteum. The structures of the preovulatory follicle and oocyte-trapped CL were also counted in each slice. The number of follicles was statistically analyzed by using Graphpad prism 6.0 software.

Wang Y., Wang W., Cheng K., Geng K., Liang J., Wang P., Zhang J., Niu S., Jia L., Zhang S., Li L., Feng X., Wang C., Wang H., Zhang H, & Zhang Y. (2022). Polycomb subunit Pcgf2 mediates ovulation and fertility through transcriptional regulation progesterone receptor. Frontiers in Cell and Developmental Biology, 10, 1010601.

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Comprehensive Ovarian Follicle Analysis

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For morphological analysis and fluorescent detection at the tissue level, ovarian samples were fixed in 4% paraformaldehyde (PFA, Santa Cruz, 30525-89-4) for 8 h, embedded in paraffin, and sectioned serially at 5 μm. To count the follicle number, tissue sections were stained with hematoxylin (Santa Cruz, sc-24973A). Primordial follicles were counted in every fifth section and multiplied by five to calculate the number of all primordial follicles in each ovary. The growing follicles were counted by scanning all sections and only the follicles with clear oocyte nuclei were counted to exclude the effect of residual structures after oocyte ablation in the ovaries. The number of primordial follicles and growing follicles was summed to the total number of follicles. For fluorescent detection, after deparaffinization, sections were stained with different antibodies and the Hoechst 33342 (Sigma-Aldrich, 14533) was used as a counter-stain to check the cell nucleus. The sections were sealed with an anti-fade fluorescence mounting medium (Applygen, C1210) by coverslips.

Zhang Y., Wang Y., Feng X., Zhang S., Xu X., Li L., Niu S., Bo Y., Wang C., Li Z., Xia G, & Zhang H. (2021). Oocyte-derived microvilli control female fertility by optimizing ovarian follicle selection in mice. Nature Communications, 12, 2523.

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Histological Analysis of Ovarian Tissue

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For morphological analysis, ovaries and tumor tissues were fixed in 4% paraformaldehyde (Santa Cruz, 30525-89-4) for 8–24 hours (according to their sizes) at 4 °C, embedded in paraffin, and sectioned serially at 5–8 μm. Sections were stained with hematoxylin (Santa Cruz, sc-24973A) for further histological analysis.

Niu S., Cheng K., Jia L., Liang J., Mu L., Wang Y., Yang X., Yang C., Zhang Y., Wang C., Huang L., Wang H., Zhang S, & Zhang H. (2023). Lineage tracing of mutant granulosa cells reveals in vivo protective mechanisms that prevent granulosa cell tumorigenesis. Cell Death and Differentiation, 30(5), 1235-1246.

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