Il 17a gfp

Inbred C57BL/6 (WT), Mir92a^–/–, Foxp3^gfp, Il17a^gfp, Ifng^yfp, B6.SJL-Ptprc^aPepc^b/BoyJ (CD45.1), and B6.129S7-Rag1^tm1Mom/J (Rag1^–/–) mice were obtained from The Jackson Laboratory. Mir92a^–/– mice had been backcrossed with mice on a C57BL/6 background for 10 or more generations before they were deposited with The Jackson Laboratory by the donating investigator. Mir92a^–/–Foxp3^gfp and Mir92a^–/–Il17a^gfp mice were generated in-house by crossing Mir92a^–/– mice with Foxp3^gfp and Il17a^gfp mice, respectively. All mice were age matched (8–12 weeks old at the start of the experiments) and sex matched. Littermate controls were used when appropriate. Mice were maintained in specific pathogen–free animal facilities at the Harvard Institutes of Medicine at Harvard Medical School and the Hale Building for Transformative Medicine at Brigham and Women’s Hospital. Mice were maintained in 20°C–25°C, 50%–70% humidity and on a 12-hour light cycle, with the light phase beginning at 7 am and ending at 7 pm. Mice were housed with ad libitum access to food and water.

DsRed B6, OT1 B6, Cd45.1 B6, OT-II B6, Thy1.1 B6, m-OVA B6, Rag1^−/− B6, Il17a-GFP, Aire^−/− B6, B6.lpr, MPJ, MRL.lpr mice were obtained from The Jackson Laboratories. Two-month-old female mice were used unless the age and sex were otherwise specified. All mice were maintained under specific pathogen-free conditions, and all procedures were approved by the Institutional Animal Care Committee of Beth Israel Deaconess Medical Center, Harvard Medical School.