Human endometrial epithelial Ishikawa cells (ATCC) and CRL4003 cells, kindly gifted from the laboratory of Professor Haeng-Seok Song of CHA University, were maintained in DMEM/F12 media (Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (FBS; Gibco, Grand Island, NY, USA) and 1% penicillin–streptomycin (Gibco, Grand Island, NY, USA) as previously described32 (link). For tube formation and sprouting assays, Human Umbilical Vein Cells (HUVEC, ATCC) and Human Endometrial Microvascular Endothelial Cells (HEMEC, PromoCell) were maintained in EGM-2 endometrial cell growth medium 2 (Lonza) and Endothelial Cell Growth Medium MV (PromoCell), respectively. Human recombinant CXCL12 (100 ng/ml, Peprotech, USA) was used for further analyses.
Hemec
Manufactured by PromoCell
HEMEC is a laboratory equipment designed for hematology analysis. It performs precise quantitative analysis of red blood cells, white blood cells, and platelets in blood samples.
Automatically generated - may contain errors
Lab products found in correlation
Endothelial cell growth medium mv, by PromoCell (2 mentions)
Recombinant human cxcl12, by Thermo Fisher Scientific (2 mentions)
Dmem f12 media, by Thermo Fisher Scientific (2 mentions)
Penicillin streptomycin, by Thermo Fisher Scientific (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Egm tm 2 endometrial cell growth medium 2, by Lonza (1 mentions)
2 protocols using hemec
1
Endometrial Cell Culture and Angiogenesis Assays
2
Endometrial Epithelial and Endothelial Cell Culture
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Human endometrial epithelial Ishikawa cells (ATCC) were maintained in DMEM/F12 media (Gibco, Grand Island, NY, USA) supplemented with 10% fetal bovine serum (Gibco, Grand Island, NY, USA) and 1% penicillin-streptomycin (Gibco, Grand Island, NY, USA) as previously described [32] . For tube formation and sprouting assays, Human Umbilical Vein Cells (HUVEC, ATCC) and Human Endometrial Microvascular Endothelial Cells (HEMEC, PromoCell) were maintained in EGM TM -2 endometrial cell growth medium 2 (Lonza) and Endothelial Cell Growth Medium MV (PromoCell), respectively. Human recombinant CXCL12 (100 ng/ml, Peprotech, USA) was used for further analyses.
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