10 kda microcon centrifugal filter devices

Manufactured by Merck Group

The 10-kDa Microcon centrifugal filter devices are a type of laboratory equipment designed for sample concentration and purification. These devices utilize a semi-permeable membrane with a molecular weight cutoff of 10 kilodaltons to separate and concentrate target molecules from a solution during centrifugation.

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Lab products found in correlation

Sequencing grade modified trypsin, by Promega (2 mentions) Endoproteinase glu c glu c, by Promega (2 mentions) Pngase f, by New England Biolabs (2 mentions) Human plasma fibronectin, by Prolytix (1 mentions) Empore c8 disk, by 3M (1 mentions) Dithiothreitol (dtt), by Thermo Fisher Scientific (1 mentions) Iodoacetamide, by Thermo Fisher Scientific (1 mentions) Ammonium hydrogen carbonate nh4hco3, by Merck Group (1 mentions)

Spelling variants of this product

Centrifugal filter (122 citations) Centrifugal filter devices (50 citations) Microcon centrifugal filters (40 citations) Microcon centrifugal filter device (27 citations) Microcon filter (20 citations) 10-kDa centrifugal filter device (2 citations) 10 kDa microcon (2 citations) Microcons (2 citations)

2 protocols using 10 kda microcon centrifugal filter devices

Fibronectin Proteome Characterization

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Reagents, solvents, and chemicals were all from Sigma-Aldrich (St. Louis, MO) unless otherwise stated. Human plasma fibronectin with a purity of a minimum 90% was purchased from Haematologic Technologies (Essex Junction, VT). Trypsin (sequencing grade modified) and endoproteinase Glu-C (Glu-C) were from Promega (Madison, WI). PNGase F was obtained from New England Biolabs (Beverly, MA). The 10-kDa Microcon centrifugal filter devices were purchased from Millipore (Bedford, MA). The ZIC-HILIC material was from SeQuant (Umea, Sweden). The OpeRATOR/SialEXO kit was from Genovis, Inc. (Cambridge, MA).

Liu D., Wang S., Zhang J., Xiao W., Miao C.H., Konkle B.A., Wan X.F, & Li L. (2021). Site-Specific N- and O-Glycosylation Analysis of Human Plasma Fibronectin. Frontiers in Chemistry, 9, 691217.

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N-Glycan Purification and Characterization

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Human pdFV was purchased from Haematologic Technologies. Trypsin (sequencing grade modified) and endoproteinase Glu-C (Glu-C) were supplied by Promega. PNGase F was obtained from New England Biolabs. Sodium cyanoborohydride (NaCNBH3), formic acid, ammonium hydrogen carbonate (NH₄HCO₃), and high-performance liquid chromatography grade acetonitrile (ACN) were purchased from Sigma-Aldrich. Dithiothreitol and iodoacetamide were obtained from Thermo Fisher. The 10-kDa Microcon centrifugal filter devices were purchased from Millipore. ZIC-HILIC material was bought from SeQuant; the 3M Empore C8 disk was purchased from 3M Bioanalytical Technologies; 10 μL extended pipet tips were from Axygen. The OpeRATOR/SialEXO kit was from Genovis, Inc. All chemicals used in the preparation of buffers and solutions were of analytical grade or better.

Ma C., Liu D., Li D., Zhang J., Xu X.Q., Zhu H., Wan X.F., Miao C.H., Konkle B.A., Onigman P., Xiao W, & Li L. (2020). Comprehensive N- and O-glycosylation mapping of human coagulation factor V. Journal of thrombosis and haemostasis : JTH, 18(8), 1884-1892.

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