V 550 uv vis spectrophotometer

Manufactured by Jasco

Sourced in Japan, United States, Italy

The V-550 UV/VIS spectrophotometer is a laboratory instrument designed for the measurement of light absorption or transmission in the ultraviolet and visible light spectrum. It can be used to analyze and quantify the properties of various samples, such as solutions, suspensions, or solid materials.

Automatically generated - may contain errors

Lab products found in correlation

6210 esi tof mass spectrometer, by Agilent Technologies (4 mentions) P 1020 polarimeter, by Jasco (4 mentions) Av 400 spectrometer, by Bruker (4 mentions) Fp 6500 spectrofluorometer, by Jasco (3 mentions) Sephadex lh 20, by GE Healthcare (2 mentions) Silica gel, by Qingdao Marine Chemical (2 mentions) Uv lamp, by BioComp Instruments (2 mentions) Ft ir 480 plus ft ir spectrometer, by Jasco (2 mentions) J 810 spectropolarimeter, by Jasco (2 mentions) G1311c pump, by Agilent Technologies (1 mentions) J 810 spectrometer, by Jasco (1 mentions) Fp 6500 spectrofluorimeter, by Jasco (1 mentions) Phenolic compound standards, by Merck Group (1 mentions) Formic acid, by Carlo Erba (1 mentions) Mts cell proliferation assay kit, by Promega (1 mentions) Z 383 k refrigerated centrifuge, by Hermle (1 mentions) Cary eclipse spectrofluorometer, by Agilent Technologies (1 mentions) 3 mm pathlength quartz cuvette, by Hellma (1 mentions) Catechin, by Merck Group (1 mentions) 2 2 diphenyl 1 picrylhydrazyl (dpph), by Merck Group (1 mentions)

Spelling variants of this product

V-550 (111 citations) V-550 spectrophotometer (75 citations) UV-Vis spectrophotometer (66 citations) UV-550 (18 citations) V-550 UV/VIS (14 citations) UV spectrophotometer (11 citations) UV-550 spectrophotometer (4 citations) V-550 ultraviolet/visible (UV/Vis) spectrophotometer (2 citations) UV-Vis V-550 double-beam spectrophotometer (2 citations)

67 protocols using v 550 uv vis spectrophotometer

Spectrophotometric Evaluation of UV Protection

Check if the same lab product or an alternative is used in the 5 most similar protocols

Changes of the physical properties as transmittance (%T) of samples occurring during modifications were assessed using double beam Jasco V-550 UV-VIS spectrophotometer (Tokyo, Japan) with integrating sphere attachment in the range of 200–800 nm, analogously as we described earlier [65 (link)]. The same apparatus was used to determine the ultraviolet protection factor (UPF) of samples.
The UPF value of the samples was determined, according to EN 13758-1:2002 standard [93 ], as the arithmetic mean of the UPF values (Equation (2)) for each of the samples (a confidence interval of 95%), analogously, as described earlier [67 (link)].

U P F = \frac{\int_{290}^{400} E (λ) ε (λ) d (λ)}{\int_{290}^{400} E (λ) ε (λ) T (λ) d (λ)}

where:
E(λ)—the solar irradiance;
ε(λ)—the erythema action spectrum (measure of the harmfulness of UV radiation for human skin);
Δλ—the wavelength interval of the measurements;
T(λ)—the spectral transmittance at wavelength λ.

Kudzin M.H., Boguń M., Mrozińska Z, & Kaczmarek A. (2020). Physical Properties, Chemical Analysis, and Evaluation of Antimicrobial Response of New Polylactide/Alginate/Copper Composite Materials. Marine Drugs, 18(12), 660.

+ Open protocol

+ Expand

Isolation and Characterization of Natural Products

Check if the same lab product or an alternative is used in the 5 most similar protocols

Optical rotations were obtained on a JASCO P-1020 polarimeter. UV spectra were recorded using a JASCO V-550 UV/VIS spectrophotometer. CD spectra were measured on a JASCO J-810 spectrometer. 1D and 2D NMR spectra were recorded on Bruker AV-500 NMR spectrometers with TMS as an internal standard. HRESIMS analyses were recorded on an Agilent 6210 ESI/TOF mass spectrometer. Column chromatography (CC) was performed with Silica gel (Qingdao Marine Chemical Plant, Qingdao, P. R. China), ODS (50 μm, YMC, Kyoto, Japan) and Sephadex LH-20 (Pharmacia Biotech, Uppsala, Sweden). Preparative HPLC was conducted on a Cosmosil C₁₈ preparative column (5 μm, 20 × 250 mm) equipped with a G1311C pump and a G1315D photodiode array detector (Agilent Technologies, CA, USA). All chemical reagents were purchased from Tianjin Damao Chemical Company (Tianjin, P. R. China).

Wu Z.N., Niu Q.W., Zhang Y.B., Luo D., Li Q.G., Li Y.Y., Kuang G.K., He L.J., Wang G.C, & Li Y.L. (2019). Hyperpatulones A–F, polycyclic polyprenylated acylphloroglucinols from Hypericum patulum and their cytotoxic activities. RSC Advances, 9(14), 7961-7966.

+ Open protocol

+ Expand

Spectroscopic Characterization of Biomolecules

Check if the same lab product or an alternative is used in the 5 most similar protocols

The UV-VIS absorption spectra were recorded on a double-beam Jasco V550UV-VIS spectrophotometer in the 400-750 nm wavelengths range with a 2 nm resolution, using 1 mm length quartz cells. Baseline corrections were run successively in air and phosphate buffer saline. Fluorescence emission and excitation spectra were measured using a Jasco FP-6500 spectrofluorimeter, equipped with a Xe lamp of 150 W, 1800 lines/mm monochromator and 1 nm spectral resolution.

Fălămaș A., Porav S.A., & Toşa V. (2020). Investigations of the Energy Transfer in the Phycobilisome Antenna of Arthrospira platensis Using Femtosecond Spectroscopy. Applied Sciences, 10(11), 4045-4045.

+ Open protocol

+ Expand

Purification and Characterization of Plasmodium falciparum Aldolase

Check if the same lab product or an alternative is used in the 5 most similar protocols

The production of GST-tagged Pf aldolase from sequence inserted into pGEX-5X and transformed into BL21 competent cells (Stratagene) has been described previously [25] (link). Pf aldolase, with the GST tag removed, was purified and its correct folding confirmed by activity measurements in which fructose 1,6-bisphosphate (F1,6P) cleavage was coupled to the triose-phosphateisomerase/α-glycerophosphate dehydrogenase reaction with continuous measurement of NADH consumption monitored at 340 nm (JASCO V550 UV/VIS Spectrophotometer), using an established method [30] (link). Kinetic analysis was performed using the Lineweaver–Burk plot to calculate the Michaelis–Menten constant (K_m) and the maximum reaction velocity (V_max) for the reaction [31] .

Diaz S.A., Martin S.R., Grainger M., Howell S.A., Green J.L, & Holder A.A. (2014). Plasmodium falciparum aldolase and the C-terminal cytoplasmic domain of certain apical organellar proteins promote actin polymerization. Molecular and Biochemical Parasitology, 197(1-2), 9-14.

+ Open protocol

+ Expand

Quantifying Chlorophylls and Carotenoids in Plant Leaves

Check if the same lab product or an alternative is used in the 5 most similar protocols

Chlorophylls and carotenoids were extracted from lyophilized leaves and homogenized using liquid nitrogen with 80% acetone at a ratio of 1:100 (w/v) under stirring for 30 min. The amounts of chlorophylls and carotenoids were calculated by applying the formula in [54 (link)] and the absorbance was read with a JASCO V-550 UV/VIS spectrophotometer (JASCO Corporation 2967-5, Ishikawa-machi, Hachioji-shi Tokyo, Japan). The results were expressed in mg·g⁻¹ dry weight (DW), and all measurements were performed in triplicate for each analyzed sample (n = 5, BN-positive and BN-negative plants, respectively).
The content of soluble sugars, expressed as mg·g⁻¹ DW, was calculated using a commercial enzymatic kit from Megazyme (Megazyme International Ltd., Ireland, cat. no. K-SUFRG 06/14), according to the manufacturer’s protocol.

Negro C., Sabella E., Nicolì F., Pierro R., Materazzi A., Panattoni A., Aprile A., Nutricati E., Vergine M., Miceli A., De Bellis L, & Luvisi A. (2020). Biochemical Changes in Leaves of Vitis vinifera cv. Sangiovese Infected by Bois Noir Phytoplasma. Pathogens, 9(4), 269.

+ Open protocol

+ Expand

Profiling Phytochemical Composition

Check if the same lab product or an alternative is used in the 5 most similar protocols

The collected leaves were lyophilized and ground with a mortar and pestle in liquid nitrogen to which the extraction buffer (methanol:water:formic acid, 60:39.9:0.1) at a ratio of 1:10 was added, and left to stir in the dark for 2 h. After centrifugation at a maximum speed (5000× g), the resulting solutions were filtered into glass vials using a 0.2 µm polytetrafluoroethylene (PFTE) membrane and analyzed as described below. Three replicates for each harvested sample (n = 5, BN-positive, and BN-negative plants, respectively) were carried out.
The total phenolic content (TPC) was determined using the spectrophotometric Folin–Ciocalteau method [55 ] and the data were expressed as mg of caffeic acid equivalent (CAE)·g⁻¹ DW.
The total flavonoid content (TFC) and amount of proanthocyanidins (PAs) were evaluated as reported by [56 ] and the absorbance was read with a JASCO V-550 UV/VIS spectrophotometer. The TFC amount was calculated by determining the absorbance at 280 and 540 nm and reported as mg of catechin equivalent (CE)·g⁻¹ DW. The proanthocyanidin (PA) quantification was measured before and after hydrolysis into cyanidins (HCl 12 N with 300 mg·L⁻¹ of FeSO₄x7H₂O for 50 min in a thermostatic bath at 100 °C at reflux). The results were expressed as mg·g⁻¹ DW.

+ Open protocol

+ Expand

Antioxidant Capacity Determination Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Phenolic compound standards, 6-hydroxy-2,5,7,8-tetramethylchroman-2-carboxylic acid (trolox), 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), 2,4,6-tri(2-pyridyl)-S-triazine (TPTZ), Folin-Ciocalteau phenol reagent were purchased from Sigma (Milan, Italy). Methanol and formic acid were obtained from Carlo Erba (Milan, Italy). All MS/MS reagents were from Bio-Rad (Hercules, CA, U.S.A.). Chemicals and enzymes for the digestion procedure were purchased from Sigma-Aldrich (Milan, Italy). All the materials and chemicals for cell culture were from Euroclone (Milan, Italy). MTS cell proliferation assay kit was purchased from Promega (Milan, Italy). Solid phase extraction (SPE) column (C18, 50 μm, 60 Å, 500 mg) were supplied by Waters (Milan, Italy).
The absorbance was read using a Jasco V-550 UV/Vis spectrophotometer.

Martini S., Conte A, & Tagliazucchi D. (2019). Bioactivity and cell metabolism of in vitro digested sweet cherry (Prunus avium) phenolic compounds. International journal of food sciences and nutrition, 70(3).

+ Open protocol

+ Expand

Preparation of S. marcescens CH-1 for Microscopy

Check if the same lab product or an alternative is used in the 5 most similar protocols

Stock cultures of S. marcescens CH-1 were maintained at -80 °C. S. marcescens CH-1 used in this work was grown to mid-log phase in Terrific Broth (Difco Laboratories; 10 mL) at 37 °C for 7 hours in the presence of 50 μg/mL kanamycin A. Bacterial concentrations were determined by measuring the absorbance of the culture at 600 nm (0.1 × O.D.₆₀₀ = 10⁸ cells/mL) in a spectrophotometer (Jasco V-550 UV-VIS spectrophotometer). The final bacterial concentration is approximately (5–9) × 10⁹ cells/mL. Before the experiment, the bacteria culture was centrifuged at 2050 × g (4510 rpm) for 5 min at 4 °C. The wet pellet was then re-suspended in distilled water, and recentrifuged (2x) to remove the growth medium. Final pellet was suspended in PBS solution. A drop of 0.01 % (wt/vol) poly-L-lysine hydrobromide solution (a positively charged compound), was added on to the mica and incubated for 30 min followed by washing with Milli-Q water before the introduction of the specimens. After washing once with PBS, the S. marcescens CH-1 cells were then placed in contact with the mica that had been coated with poly-L-lysine hydrobromide. S. marcescens CH-1 cells were attached to the mica through electrostatic interactions (physical adsorption).

Lin Y.C., Huang C, & Lai H.C. (2019). Revealing the ultrastructure of the membrane pores of intact Serratia marcescens cells by atomic force microscopy. Heliyon, 5(10), e02636.

+ Open protocol

+ Expand

ACE-inhibitory Activity Measurement Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

ACE-inhibitory activity was measured by the spectrophotometric assay of Ronca-Testoni (1983) using the tripeptide, N-[3-(2-furyl)acryloyl]-L-phenylalanyl-glycyl-glycine (FAPGG) as substrate.
The reaction was monitored at 345 nm for 10 min. For the calculation of the IC50 value, the ACE assay was carried out in presence of different amounts of the un-fractionated samples collected during the gastric and pancreatic phases of the digestion and of the lower and higher of 3 kDa fractions of the post-pancreatic sample. IC50 was defined as the concentration of peptides required to inhibit 50% of the enzymatic activity.
The absorbance was read using the same Jasco V-550 UV/Vis spectrophotometer as reported in paragraph 2.4.

Tagliazucchi D., Shamsia S., Helal A, & Conte A. (2017). Angiotensin-converting enzyme inhibitory peptides from goats' milk released by in vitro gastro-intestinal digestion. International Dairy Journal., 71.

+ Open protocol

+ Expand

Quantification of Peptide Content in Goat Milk

Check if the same lab product or an alternative is used in the 5 most similar protocols

Where h is the hydrolysis equivalent, defined as the concentration in milliequivalents/g of protein of α-amino groups formed at the different stages of the simulated digestion, and htot is the hydrolysis equivalent (total number of amino groups) at complete hydrolysis to amino acids. The total number of amino groups was determined by hydrolysing skimmed goat milk in 6 mol L -1 HCl at 110°C for 24 h. The htot value was calculated resulting in 8.6 milliequivalents per gram of protein. DH data were subtracted with the data obtained in the control digestion.
Low molecular weight peptides were extracted by ultrafiltration from the post-pancreatic digested samples (corresponding to the aliquots collected after 120 min of pancreatic digestion). Briefly, 4 mL of sample were loaded on an Amicon Ultra-4 nominal filter (cut-off 3 kDa) and centrifuged at 7500g for 120 min at 4°C using a Hermle Z383K refrigerated centrifuge (HERMLE Labortechnik GmbH, Wehingen, Germany). The filtrates, containing low molecular weight peptides, were collected and freeze-dried. The peptide content of the filtrates was determined by using the TNBS method as described above and expressing the results as mg of leucine equivalent mL -1 .
The absorbance was read using a Jasco V-550 UV/Vis spectrophotometer (Orlando FL, U.S.A.).

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!