ELISA kits with colorimetric output were used to determine the plasma concentrations of PAI-1 (ab184863, Abcam), fibrinogen (ab241383, Abcam), plasminogen (ab108893, Abcam), tissue plasminogen activator (t-PA) (ab190812, Abcam), platelet factor 4 (PF4) (ab189573, Abcam), interleukin (IL) 1 receptor-like 1 (ST2) (ab254505, Abcam), von Willebrand factor (vWF) (ab223864, Abcam), and tryptase (EKU10581, Biomatik) according to the manufacturer’s instructions. The plates were read at 450 nm using an automated microplate reader (Biotek Epoch). For each subject, we report the mean value from at least two replicate determinations with a coefficient of variation (CV) lower than 20%. The mean intra-assay CV was lower than 10% for all biomarkers and the inter-assay CVs were 11% for PAI-1, 10% for fibrinogen, 7% for plasminogen, 8% for t-PA, 9% for PF4, 14% for ST2, 10% for vWF, and 12% for tryptase. The plasma concentrations of each biomarker were determined using a standard curve, constructed and fit using a 4P logistic regression equation, and the assay values were corrected by the appropriate dilution factor in each case.
Ab108893
Manufactured by Abcam
Ab108893 is a primary antibody produced by Abcam. It is designed for use in immunohistochemistry and western blotting applications.
Automatically generated - may contain errors
3 protocols using ab108893
1
Multiplex Biomarker Quantification in Plasma
2
Quantification of Human Plasminogen and PAI-1
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3
Quantifying Secreted Proteins in Cell Culture
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Human ELISA kits, including PAI‐1 (BMS2033, Thermo Fisher Scientific) and plasminogen (ab108893, Abcam), were used according to the manufacturer's instructions. Briefly, MSG‐treated cell culture media were harvested via centrifugation at 2122 g for 10 min at 4°C to remove debris. The resulting supernatants were collected and used in the experiments.
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