Actin β polyclonal antibody

Quercetin, kaempferol, isorhamnetin, glycyrrhetinic acid, liquiritin, and glycyrrhizic acid ammonium salt were obtained from the National Institutes for Food and Drug Control (Beijing, China). Naringenin and licochalcone A were offered from Nanjing Spring and Autumn Biotech (Nanjing, China). Lipopolysaccharides (LPS; Escherichia coli 055: B5) were purchased from Solarbio LIFE SCIENCES (Beijing, China). The purity of each reference standard was above 98%. Cell Counting Kit 8 (CCK-8) was obtained from Dojindo Laboratory (Kumamoto, Japan). Fetal bovine serum (FBS) and RPMI 1640 medium were products of Biological Industries (Kibbutz Beit Haemek, Israel). Antibodies against Akt, Phospho-Akt (Ser473), Bcl-2, Bax and NF-κB p65 were supplied by Cell Signaling Technology (Beverly, MA, United States). Antibody for active + pro Caspase-3 was purchased from ABclonal Technology Co., Ltd. (Wuhan, China). Actin β Polyclonal Antibody was purchased from Immunoway (Suzhou, China). The Annexin V fluorescein isothiocyanate (FITC)/Propidium Iodide (PI) apoptosis kit was provided by the Beyotime Institute of Biotechnology (Shanghai, China). All other chemicals were provided by Sigma-Aldrich Co. (Shanghai, China), unless otherwise indicated.

PTER (SP9570, purity ≥98%) was purchased from Beijing Solarbio Science and Technology Co., Ltd. (Beijing, China). LPS (L2880, purity >97%) was purchased from the Sigma Chemical Co. (St. Louis, MO, United States). MPO (A044-1–1), SOD (A001-3–2), CAT (A007-1–1), GSH-Px (A005-1–2) and MDA (A003-1–2) Assay Kit and were purchased from Nanjing Jiancheng Bioengineering Institute (Nanjing, China). TNF-α, IL-6, IL-1β, COX-2 and iNOS primers were purchased from Sangon Biotech Co., Ltd. (Shanghai, China). p-p65 rabbit polyclonal antibody (A13599) was acquired from Boster Biological Technology co.Itd. p65 rabbit polyclonal antibody (BS9879M), p-IκB rabbit polyclonal antibody (BS4105), IκB rabbit polyclonal antibody (BS3601), Nrf2 rabbit polyclonal antibody (BS1258) and HO-1 rabbit polyclonal antibody (BS6626) were acquired from Bioworld Technology, Inc (Minnesota, United States). Actin-β polyclonal antibody (YT0099) was acquired from ImmunoWay Biotechnology Company (Plano, TX, United States). All other chemicals were at the reagent level.