RiboTag mice were purchased from The Jackson Laboratory (Stock No. 011029). This mouse line, generated by the McKnight lab (33 (link)), carries a floxed WT C-terminal exon in the Rpl22 gene, followed by a mutant exon that has a triple haemagglutinin (HA) epitope inserted in front of the stop codon, leading to the generation of HA-tagged ribosomes when crossed with a Cre-expressing mouse strain. The RiboTag mice were first crossed with the E2a-Cre mouse line, also purchased from The Jackson Laboratory (Stock No. 003724), and bred to homozygous genotype, ensuring the excision of the WT C-terminal exon and the expression of HA-tagged Rpl22 in all tissues. These mice were then crossed with the Rpl3l−/− strain and bred to obtain a homozygous genotype.
Ribotag mice
Manufactured by Jackson ImmunoResearch
Sourced in United States, Montenegro
RiboTag mice are a genetically engineered mouse model that allows for the isolation of cell type-specific ribosomes and associated mRNA. The RiboTag system utilizes a Cre recombinase-dependent strategy to express an epitope-tagged ribosomal protein, enabling the immunoprecipitation of ribosomes and the recovery of cell type-specific mRNA transcripts.
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Lab products found in correlation
Alb cre mice, by Jackson ImmunoResearch (2 mentions)
Wt c57bl 6 mice, by Jackson ImmunoResearch (1 mentions)
Rictorflox flox, by Jackson ImmunoResearch (1 mentions)
Mtorflox flox, by Jackson ImmunoResearch (1 mentions)
Cmv cre mice, by Jackson ImmunoResearch (1 mentions)
Vevo 2100 imaging system, by Fujifilm (1 mentions)
Tdtomato reporter mice, by Jackson ImmunoResearch (1 mentions)
Pv cre mice, by Jackson ImmunoResearch (1 mentions)
13 protocols using ribotag mice
1
Generating HA-tagged Ribosomes in Mice
2
Transgenic Mouse Lines for Neuronal Labeling
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SST-Cre mice (013044), PV-Cre mice (012358), Rosa 26 reporter mice (006148), Ai14 reporter mice (007914), and Ribotag mice (011029) were purchased from The Jackson Laboratory (CA, USA). LhX6-EGFP mice (000246-MU) were purchased from Mutant Mouse Resource & Research Centers (MMRRC). These mice were bred to C57BL/6 J for more than 6 generations. SST-Cre::EYFP or PV-Cre::EYFP alleles were generated by crossing SST-Cre or PV-Cre mice with Rosa 26 reporter mice; SST-Cre::RPL22-HA or PV-Cre:: RPL22-HA alleles were generated by crossing SST-Cre or PV-Cre mice with Ribotag mice; SST-Cre::tdTomato alleles were generated by crossing SST-Cre mice with Ai14 mice; LhX6-EGFP/SST-Cre::tdTomato mice were generated by crossing LhX6-EGFP mice with SST-Cre::tdTomato mice; LhX6-EGFP/SST-Cre were generated by crossing LhX6-EGFP mice with SST-Cre mice. 6–10-week-old male offsprings were used in the experiments, and randomly assigned to groups. Mice used for the experiments were housed in groups on a 12 h light/dark cycle (light on from 8 a.m. to 8 p.m.) with access to food and water ad libitum. All experiment procedures were strictly in accordance with the National Institutes of Health Guide for the Care and Use of Laboratory Animals, and were approved by Animal Care and Use Committee of the animal facility at Fudan University.
3
Genetically Engineered Mouse Models
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Rictorflox/flox, Rptorflox/flox, Mtorflox/flox and RiboTagmice with C57BL/6 background and C57BL/6 WT mice were purchased from Jackson Laboratories (Bar Harbor, Maine). Gsk3bflox/flox and Gsk3aflox/flox mice with C57BL/6 background were originally developed by Dr. Jim Woodgett (Doble et al., 2007 (link); Patel et al., 2008 (link)) and were acquired from Dr. Thomas Force. We crossed them to generate Gsk3a/bflox/flox mice. All experimental procedures were performed in compliance with animal protocols approved by the IACUC at Temple University School of Medicine. For all surgical and treatment comparisons, control and treatment groups were prepared together in single cohorts, and the experiment repeated at least twice.
4
Cardiac Ribo-tag Mouse Model for TAC
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All experiments were performed in 9-week-old male mice. The Ribo-tag mice were purchased from Jackson Laboratory (JAX ID 011029). The Ribo-tag mouse was bred to the αMHC-Cre mice or to Cdh5-CreERT2 mouse lines to obtain cardiac myocyte or endothelial Rpl22HA homozygous mice, respectively. Transverse aortic constriction (TAC) surgery was performed as previously described.14 (link) Animals were randomly assigned to the experimental groups. Sham-operated mice were euthanized at time points matching to TAC surgery time points. Institutional Animal Care and Use Committee approval was obtained for all animal studies.
5
Ribosomal Tagging in Transgenic Mice
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Mice were maintained on a 12-h light/12-h dark cycle. The RiboTag mice (Stock No. 011029, Jackson Laboratory, Bar Harbor, ME) and CMV-Cre mice (Stock No. 006054, Jackson Laboratory, Bar Harbor, ME) were purchased from Jackson Laboratory. The RiboTag mice were bred to the CMV-Cre mice to obtain homozygous mice constitutively expressing Rpl22-HA. Once the model of Rpl22-HA-expressing homozygous mice was built successfully, we maintained the colony as a separate mouse line.
6
Ribosome Profiling in Liver Cells
7
Ribosome Profiling in Liver Cells
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The RiboTag mice and the Alb-Cre mice were purchased from Jackson Laboratory. Mice were housed in a temperature- and humidity- controlled facility with a 12-h light/dark cycle. The RiboTag mouse was bred to the Alb-Cre mouse line to obtain Rpl22HA-expressing homozygous mice. Mice at the age of 8-12 weeks were subjected to overnight fasting. All animal procedures were approved by Cornell IACUC (#2008-0167).
8
Cardiac Hypertrophy Model in Mice
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All experiments were performed in 10-wk-old male mice unless otherwise indicated. The RiboTag mice were purchased from the Jackson Laboratory (JAX ID 011029). The mice were housed in a temperature- and humidity-controlled facility with a 12-h light–dark cycle. The RiboTag mouse was bred to the αMHC-Cre mice to obtain Rpl22HA-expressing homozygous mice in cardiac myocytes. At 10 wk of age, male mice underwent TAC (27 gauge needle) or sham operation, as previously described (Rockman et al, 1991 (link)) (Doroudgar et al, 2015 (link)). For echocardiography, the mice were anesthetized with 2% isoflurane and scanned using a Vevo2100 imaging system (Visual Sonics) as previously described (Völkers et al, 2014 (link)). Institutional Animal Care and Use Committee approval was obtained for all animal studies.
9
Ribotagging Astrocytes in Mice
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RiboTag mice (9 (link)) were purchased from The Jackson Laboratory. Mice expressing HA-tagged ribosomal protein RPL22 in astrocytes were generated by crossing RiboTag mice with GFAP-Cre mice (13 (link)). All animal experiments were approved by the University of California, Los Angeles Animal Research Committee. Informed consent was attained for research involving human autopsy tissues.
10
Generating Adad2-Ribotag Mice
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All animal use protocols were approved by the Rutgers University animal care and use committees. Mouse procedures were conducted according to relevant national and international guidelines (AALAC and IACUC). Generation of Adad2M/M mice was as described by Chukrallah et al. (2020) (link). RiboTag mice (Sanz et al., 2009 (link)) were obtained from The Jackson Laboratory and Adad2-Ribotag mice were generated as described by Chukrallah et al. (2020) (link). Mice were housed in a sterile, climate-controlled facility on a 12 h light cycle. Mice were fed LabDiet 5058 irradiated rodent chow and had access to food and water ad libitum.
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