All aortic tissue cross-sections from the above experiments were prepared with a Bond-Max autostainer (Leica Microsystems, Wetzlar, Germany). Slides were stained with primary antibody on a fully automated Bond-Max system and VBS Refine polymer detection system (Leica Microsystems) as previously described (Lo et al., 2017 (link)). Negative controls did not contain primary antibody. Positive immunoreactivity signals in the endothelial layers were measured by ImageJ software. Immunoreactivity signals in endothelial layers from TNF-α and the TNF-α+LSM, TNF-α+LSM+Ab, or TNF-α+rbFGF groups were quantified as previously described (Federici et al., 2002 (link)).
Vbs refine polymer detection system
Manufactured by Leica
The VBS Refine polymer detection system is a laboratory equipment product offered by Leica. It is designed to detect and analyze polymers in various samples. The system provides accurate and reliable results for polymer identification and characterization.
Automatically generated - may contain errors
3 protocols using vbs refine polymer detection system
1
Immunohistochemical analysis of aortic tissue
2
Aortic Tissue Immunohistochemistry in db/db Mice
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For aortic tissue sections from db/db mice, 3,3′-diaminobenzidine staining was performed using a Bond-Max autostainer (Leica Microsystems). Briefly, paraffin-embedded aortic tissue sections were placed in Tris buffered saline with Tween-20, then rehydrated through serial dilutions of alcohol, and washed with PBS (pH 7.2). Slides were then stained with primary antibodies against IRAK-1 (dilution 1:50, mouse monoclonal antibody, Santa Cruz), or ICAM-1 (dilution 1:50, mouse monoclonal antibody, Thermo Fisher), or incubated with PBS (as a negative control) on a fully automated Bond-Max system using onboard heat-induced antigen retrieval and a VBS Refine polymer detection system (Leica Microsystems).
3
Immunohistochemical Analysis of Neuroendocrine Markers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Hematoxylin and eosin staining results of all 21 samples were reviewed by two pathologists. The specimens were fixed in formalin and embedded in paraffin before they were archived. We used the archived specimens for immunohistochemical analysis. Immunohistochemical staining was performed with Bond-Max autostainer (Leica Microsystems, Wetzlar, Germany), using CD56 (NCL-CD56-1B6, clone 1B6; 1:15; Novocastra/Leica, Newcastle-upon-Tyne, UK), chromogranin (NCL-CHROM-430, clone 5H7; 1:50; Novocastra/Leica), synaptophysin (NCL-SYNAP-299, clone 27G12; 1:50; Novocastra/Leica), and neuron-specific enolase (BBS/NC/VI-H14; 1:200; Dako, Carpinteria, CA, USA). Briefly, specimens from the paraffin-embedded blocks were cut into 5-μm sections. The sections were dewaxed in a 60°C oven, deparaffinized in xylene, rehydrated through serial dilutions of alcohol, and washed in phosphate-buffered saline (pH 7.2). Immunohistochemical staining was performed in the fully automated Bond-Max autostainer using onboard, heat-induced antigen retrieval in citrate buffer according to the ER1 protocol for 20 minutes and a VBS Refine polymer detection system (Leica Microsystems). Diaminobenzidine was used as the chromogen (Leica Microsystems). The sections were then counterstained with hematoxylin.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!