Tnfr2ko

All the experimental procedures were approved by and performed in accordance with the guidelines and practices established by the Tulane University Animal Care and Use Committee.
Male mice in which the TNF‐α receptors TNFR1 (B6.129‐Tnfrsf1atm1Mak/J) and TNFR2 (B6.129S2‐Tnfrsf1btm1Mwm/J) had been genetically knocked out and their background WT (WT; C57BL/6) mice were used in these experiments (Jackson Laboratories). All these mice (n = 6 in each group; 9–10 week old with average body weights, WT, 25.7 ± 0.7 g; TNFR1KO, 24.2 ± 0.8 g; TNFR2KO, 24.9 ± 0.6 g) were purchased from Jackson Laboratories. Male mice were only used in this study as we have conducted similar acute experiments in our earlier studies which give us the advantage to compare these results with findings from other similar studies. (Castillo et al., 2012 (link); Shahid et al., 2008 (link)). These mice were housed in a temperature‐ and light‐controlled room in the Tulane Vivarium and allowed free access to standard diet (Ralston‐Purina) and tap water for ≥3 days before acute renal clearance studies were performed under anesthesia.

Eight-week-old female mice were used in this study. TNFR1-KO [6 (link)], TNFR2-KO [15 (link)], and lpr (Fas-KO) gld (FasL-KO) mice were from the Jackson Lab (Bar Harbor, ME). Perforin-deficient mice (perforin-KO) were kindly provided by Dr. H. Hengartner [23 (link)]. Wild type C57BL/6 mice were used as controls. Nuclear factor (NF)-κB deficient mice were a kind gift from Dr. Kubota, Kyushu University [24 (link)]. Because mice with a targeted disruption of the p50 subunit of NF-κB [25 (link)] were on a BALB/c background, wild type BALB/c were used as controls. In sterile food and water in an environmentally controlled room, these mice were bred.