MiR-124 mimic were purchased from GenePharma. The following Flowcytometry antibodies were purchased from BD Biosciences (USA), such as FITC-CD4 (L3T4, 553729), APC-CD25 (PC61.5, 557192), PE-IL-17 (TC11-18H10, 559502), PE-cy7-IFN-γ (XMG1.2, 561040), PE-FOXP3 (FJK-16S, 560408) and isotype controls. Antibodies for RORγ (562197) were purchased from BD Bioscience. FITC Annexin V Apoptosis DetectionKit I (2293683) was purchased from BD Pharmingen. anti-T-bet (Invitrogen,14-5825-82), anti-STAT3(cell signaling technology, 4368), anti-pSTAT3 (Cell Signaling Technology, 4074), ago2 antibody (proteintech, 10686-1-AP), and anti-β-actin (Sigma, A5441) antibodies for western blotting were used according to the manufacturers’ instructions. Secondary antibodies were from Santa Cruz Biotechnology, Inc.
Ago2 antibody
Manufactured by Proteintech
Sourced in United States
The AGO2 antibody is a laboratory tool used to detect the presence and quantify the levels of the AGO2 protein in biological samples. AGO2 is a key component of the RNA-induced silencing complex (RISC), which plays a crucial role in gene expression regulation through RNA interference (RNAi) mechanisms. This antibody can be used in various experimental techniques, such as western blotting, immunoprecipitation, and immunohistochemistry, to study the expression and localization of AGO2 in different cell types and tissues.
Automatically generated - may contain errors
Lab products found in correlation
Magna rip rna binding protein immunoprecipitation kit, by Merck Group (2 mentions)
Anti p stat3, by Cell Signaling Technology (1 mentions)
Anti stat3, by Cell Signaling Technology (1 mentions)
Mir 124 mimic, by GenePharma (1 mentions)
Flow cytometry antibodies, by BD (1 mentions)
Anti t bet, by Thermo Fisher Scientific (1 mentions)
Anti β actin, by Merck Group (1 mentions)
Fitc annexin 5 apoptosis detection kit 1, by BD (1 mentions)
Rna immunoprecipitation rip kit, by BersinBio (1 mentions)
Magna rip kit, by Merck Group (1 mentions)
Magna riptm rna binding protein immunoprecipitation kit, by Merck Group (1 mentions)
7 protocols using ago2 antibody
1
Immune Response Regulation by miR-124
2
Investigate ZFAS1 Binding to AGO2
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RIP assay was performed with Magna RIPTM RNA-Binding Protein Immunoprecipation Kit (Millipore, Billerica, USA) and AGO2 antibody (proteintech, 10686–1-AP, China) in accordance with the manufacturer’s protocol. HCT116 cells were transfected with agomiR-150-5p or agomiR-150-5p negative control (agomiR-NC), after 48 h, cells were performed RIP using AGO2 antibody and then relative ZFAS1 expression was analyzed by qRT-PCR. The RIP fraction Ct value was normalized to the input RNA fraction Ct value.
3
Probing PD1-miR-15a-5p Interaction
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We performed RNA-binding protein immunoprecipitation (RIP) assay to determine the binding between PD1 and miR-15a-5p using Magna RIP™ RNA-Binding Protein Immunoprecipitation Kit (Millipore) and AGO2 antibody (66720-1-Ig, Proteintech) as previous study (20 (link)). Briefly, CD8+ T cells were transfected with PD1 or IgG, and the mRNA level of miR-15a-5p was detected using qRT-PCR.
4
Detecting circHAS2 and miR-944 Interaction
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The BersinBioTM RNA Immunoprecipitation (RIP) Kit (BersinBio, Guangzhou, China) was used to perform the RIP experiments. HGC-27 and MKN-45 cells overexpressing hsa-miR-944 were used. Cells were lysed in RNA lysis buffer and then incubated at 4 °C overnight with RIP buffer containing coupled Argonaute-2 (AGO2) antibody (Proteintech, Rosemont, IL, USA) or negative control IgG. Then the magnetic beads were washed three times. After proteinase K treatment, the immunoprecipitated RNA was extracted with phenol-chloroform-isopentyl alcohol (25:24:1). Finally, qPCR was used to detect the expression of circHAS2 and hsa-miR-944.
5
RNA Immunoprecipitation Protocol for AGO2
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According to the manufacturer's instructions for the Magna RIP kit (Millipore), about 2 × 107 cells were pelleted and resuspended in lysis buffer. Then, cell lysates were incubated with 5 μg of IgG (Abcam, Cambridge, UK) or AGO2 antibody (Proteintech, Wuhan, China) antibody-magnetic coated beads and rotated at 4 ℃ overnight. The purified co-precipitated RNAs were reverse-transcribed and detected by qRT-PCR.
6
RIP Assay for SNHG3 Detection
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The Magna RIPTM RNA Binding Protein Immunoprecipitation Kit (Millipore, USA) and AGO2 antibody (10686–1-AP, Proteintech, China) were utilized to perform RIP assays according to the instructions of the manufacturer. BC cells were subjected to RIP using the AGO2 antibody, and subsequent analysis involved measuring the relative expression of SNHG3 using qRT-PCR. The Ct value of the RIP fraction was normalized to that of the input RNA fraction.
7
Profiling RNA Binding Proteins in HUVECs
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RNA-binding protein immunoprecipitation (RIP) assays were performed with a Magna RIP RNA-binding protein immunoprecipitation kit, according to the manufacturer’s instruction (Millipore). HUVECs ( cells) were seeded in plates and held overnight, then the cells were exposed to PCB29-pQ for 24 h. Cells were lysed using of complete RIP lysis buffer and harvested. Magnetic beads were conjugated to the argonaute 2 (Ago2) antibody using the Magna RIP RNA-binding protein immunoprecipitation kit, according to the manufacturer’s protocol (Cat. No. 17-700). The cell lysates were then incubated with RIP buffer containing magnetic beads conjugated to Ago2 antibody (Proteintech Group, Inc.) at 4°C overnight. The removal of unbounded antibody was performed by centrifugation and washing, according to the manufacturer’s protocol (Cat. No. 17-700). The co-precipitated RNAs were isolated using TRNzol universal reagent, and the following RNAs were detected by RT-qPCR (HDAC7-AS1, RP3-416H24.1, LINC01547, TUG1, MCM3AP-AS1, and FGD5-AS1).
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