Necrosulfonamide

Manufactured by R&D Systems

Sourced in United States

Necrosulfonamide is a small molecule inhibitor that targets and blocks the activity of the protein MLKL (Mixed Lineage Kinase Domain-Like Protein). MLKL is a key mediator of necroptosis, a form of programmed cell death.

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Lab products found in correlation

P mlkl, by Abcam (2 mentions) Anti mouse igg hrp linked 7076 s, by Cell Signaling Technology (1 mentions) Dimethyl sulfoxide (dmso), by Merck Group (1 mentions) S100a9 nb110 89726, by Novus Biologicals (1 mentions) Recombinant human tnf, by R&D Systems (1 mentions) Gapdh, by Merck Group (1 mentions) Gemcitabine, by Merck Group (1 mentions) Z vad fmk, by Merck Group (1 mentions) Necrostatin 1, by Merck Group (1 mentions) P mlkl, by Cell Signaling Technology (1 mentions) C parp, by Cell Signaling Technology (1 mentions) P gsk3β, by Cell Signaling Technology (1 mentions) Staurosporine, by Selleck Chemicals (1 mentions) Kinase inhibitor library, by Selleck Chemicals (1 mentions) β actin, by Merck Group (1 mentions) Aurka, by Cell Signaling Technology (1 mentions) Aurkb, by Cell Signaling Technology (1 mentions) P aurka, by Cell Signaling Technology (1 mentions) Togpx4, by Abcam (1 mentions) Cct137690, by Selleck Chemicals (1 mentions)

Spelling variants of this product

Necrosulfonamide (NSA) (2 citations)

2 protocols using necrosulfonamide

Investigating Cell Death Signaling Pathways

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TNF-α (210-TA-005), Smac (789-SM-100), z-VAD-fmk (FMK001), Necrostatin-1s (Nec-1s), and Necrosulfonamide (NSA) were purchased from R&D (Minneapolis, MN, USA). DMSO were obtained from Sigma. GSK’872 was obtained from MCE. Following primary antibodies were used: HRP-linked anti-mouse IgG (7076 S) and anti-rabbit IgG (7074 S), Cleaved Caspase-3 (9664 S) and RIPK1 (3493 S) (Cell Signaling Technology, Danvers, MA, USA). Caspase-8 (sc-5263), TLR4 (sc-293072) and RIPK3 (sc-374639) (Santa Cruz, CA, USA). HMGB1 (ab79823), RIPK3 (ab56164), pRIPK3(S227, ab209384), pRIPK3 (S232, ab195117), MLKL (ab194699), pMLKL (S345, ab196436) and pMLKL (S358, ab187091) (Abcam, Cambridge, UK). β-actin and GAPDH (Affinity Biosciences, USA), N-cadherin (13769-1-AP) and S100A8 (15792-1-AP) (Proteintech, Rosemont, IL, USA). S100A9 (NB110-89726, Novus Biologicals, USA). Cell Counting Kit-8 (CCK-8) and cytotoxicity LDH assay kit were purchased from Dojindo China Co., Ltd (Shanghai, China). Minute ^TM Plasma Membrane Protein Isolation and Cell Fractionation Kit was purchased from Invent Biotechnologies, Inc. (Minnesota, USA).

Duan X., Liu X., Liu N., Huang Y., Jin Z., Zhang S., Ming Z, & Chen H. (2020). Inhibition of keratinocyte necroptosis mediated by RIPK1/RIPK3/MLKL provides a protective effect against psoriatic inflammation. Cell Death & Disease, 11(2), 134.

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Antibody Panel for Cell Signaling Pathways

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The antibodies to c-PARP (#9544), p-MLKL (Ser358,
#91689), MLKL (human specific, #14993), MLKL (mouse specific,
#28640), p-H3 (Ser10, #3377), GSK3β (#9315),
p-GSK3β (Ser9, #9336), RIPK3 (#13526),
p-AURKA(Thr288)/AURKB(Thr232)/AURKC(Thr198) (#2914), AURKA (human
specific, #14475), Flag (#14793), and HA (#3724) were
purchased from Cell Signaling Technology. The antibodies to GAPDH
(#G8795) and β-actin (#A5441) were purchased from Sigma
Aldrich. The antibodies to RIPK1 (#NB100-56160), LC3
(#NB100-2220), HMGB1 (#H00003146-M08), and AURKA
(#NBP2-36737) were purchased from Novus Biologicals. The antibodies to
GPX4 (#ab125066) and p-MLKL (Ser345, # ab196436) were purchased
from Abcam. The antibody to p-AURKA (Thr288, # GTX55002) was purchased
from GeneTex Inc. The antibody to TNFR1 (#MAB225R) was purchased from
R&D Systems. CCT137690 (#S2744), ferrostatin-1 (#S7243),
AR-A014418 (#S7435), staurosporine (#S1421), erastin
(#S7242), and kinase inhibitor library (#L1200) were obtained
from Selleck Chemicals. Necrostatin-1 (#N9037) and gemcitabine
(#G6423) were purchased from Sigma Aldrich. Z-VAD-FMK (#219007)
and XXVI (#361569) were purchased from EMD Millipore. Chloroquine
(#193919) was purchased from MP Biomedicals. Necrosulfonamide
(#5025) and recombinant human TNF (#210-TA-005) were purchased
from R&D Systems.

Xie Y., Zhu S., Zhong M., Yang M., Sun X., Liu J., Kroemer G., Lotze M., Zeh HJ I.I.I., Kang R, & Tang D. (2017). Inhibition of Aurora Kinase A Induces Necroptosis in Pancreatic Carcinoma. Gastroenterology, 153(5), 1429-1443.e5.

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