All chemicals and anhydrous solvents were purchased from Sigma-Aldrich, Merck, Alfa Aesar and TCI and used without further purification. Melting points (mp) were determined with SMP30 melting point apparatus in open capillaries and are uncorrected. FT-IR spectra were recorded by using Perkin Elmer Spectrum 100 FT-IR spectrometer. Nuclear Magnetic Resonance (1H-NMR and 13 C-NMR) spectra of compounds were recorded using a Bruker Advance III 300 MHz spectrometer in DMSO-d6 and TMS as an internal standard operating at 300 MHz for 1H-NMR and 75 MHz for 13 C-NMR. Thin layer chromatography (TLC) was carried out on Merck silica gel 60 F254 plates.
Advance 3 300 mhz spectrometer
Manufactured by Bruker
The Bruker Advance III 300 MHz spectrometer is a high-performance nuclear magnetic resonance (NMR) spectrometer designed for analytical and research applications. It operates at a magnetic field strength of 7.05 Tesla, providing a 300 MHz proton resonance frequency. The Advance III 300 MHz spectrometer is capable of performing a range of NMR experiments to analyze the structure and properties of chemical compounds.
Automatically generated - may contain errors
8 protocols using advance 3 300 mhz spectrometer
1
Synthesis and Characterization of Novel Compounds
2
Deuterated TALP Powder Preparation
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Deuterated TALP powders were made by soaking normal TALP in D2O (70% D, Cambridge Isotope Laboratories, Inc.) for 1 week to substitute the interlayer H2O. Prior to ssNMR measurement, the deuterated POAFs were dried overnight under vacuum at 100 °C. The ssNMR was run on a Bruker Advance III 300 MHz spectrometer with a 7 Tesla superconducting magnet operating at frequencies of 300 MHz and 46 MHz for the 1H and 2H nuclei respectively. Approximately 80 mg of sample was packed in a 4 mm zirconia rotor fitted with a Kel-F cap and spun to 6 kHz at the magic angle in a 4 mm HX double-resonance MAS probe head. The 2H NMR signal was acquired with a solid echo sequence using optimized hard 1 μs radiofrequency pulses, with 60 kHz 1H SPINAL decoupling and 1 s recycle delays. A total of 30 k signal transients were co-added to yield sufficient signal to noise and the overall signal was simulated using the Dmfit software to extract the quadrupolar parameters.
3
Synthesis and Characterization of Novel Organic Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All chemicals and anhydrous solvents were purchased from Sigma-Aldrich, Merck, Alfa Aesar and TCI and used without further purification. Melting points (mp) were determined with SMP30 melting point apparatus in open capillaries and are uncorrected. Elemental analysis was carried out on a LEO CHNS model 932 elemental analyser. FT-IR spectra were recorded by using Perkin Elmer Spectrum 100 FT-IR spectrometer. Nuclear magnetic resonance (1H NMR and 13C NMR) spectra of compounds were recorded using a Bruker Advance III 300 MHz spectrometer in DMSO-d6 and TMS as an internal standard operating at 300 MHz for 1H NMR and 75 MHz for 13C NMR. Thin layer chromatography (TLC) was carried out on Merck silica gel 60 F254 plates.
4
Synthesis and Characterization of Chitosan Oligomers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Chitosan (15 g, 83.8 mmol of GlcN unit) was dissolved in 500 mL of deionized water with the addition of 7.6 mL HCl (37% w/w). Twelve milliliters of a freshly prepared aqueous solution of NaNO2 (723 mg, 10.5 mmol for a GlcN unit/NaNO2 molar ratio equal to 8) was added and the reaction was allowed to proceed for 12 h at room temperature. The solution was filtered through a cellulose membrane (1.2 µm) and the chitosan oligomers were then precipitated by the addition of an NH4OH solution (28% w/w) to pH 9–10. The precipitate was washed several times by centrifugation with water until neutral pH and was finally freeze-dried. COSamf (9.8 g, 65% mass yield) was obtained as a white powder and stored at −20 °C. 1H NMR spectrum was recorded on a Bruker Advance III 300 MHz spectrometer at 300 K according to an analysis method previously described [23 (link)]. 1H-NMR (300 MHz, D2O) of COSamf (Figure 1 ): (ppm) 5.10 (d, J = 5.4 Hz, 1H, HI), 4.90–4.80 (m, 19H, H1), 4.45 (t, J = 4.9 Hz, 1H, HIII), 4.23 (t, J = 4.9 Hz, 1H, HIV), 4.13 (m, 1H, HV), 4.05–3.45 (m, 98H, HII and HVI, H3 to H6), 3.25–3.08 (m, 19H, H2).
5
Spectroscopic Characterization of Organic Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All chemicals and anhydrous solvents were purchased from Sigma-Aldrich, Merck, Alfa Aesar and TCI and used without further purification. FT-IR spectra were obtained by using Perkin Elmer Spectrum 100 FT-IR spectrometer. Nuclear Magnetic Resonance (1H-NMR and 13 C-NMR) spectra of compounds were recorded using a Bruker Advance III 300 MHz spectrometer in DMSO-d6 and TMS as an internal standard operating at 300 MHz for 1H-NMR and 75 MHz for 13 C-NMR. Thin-layer chromatography (TLC) was carried out on Merck silica gel 60 F254 plates.
6
Synthesis and Characterization of Triazenes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All chemicals and anhydrous solvents were purchased from Sigma-Aldrich, Merck, Alfa Aesar and TCI and used without further purification. Melting points (mp) were determined with SMP30 melting point apparatus in open capillaries and are uncorrected. FT-IR spectra were recorded by using Perkin Elmer Spectrum 100 FT-IR spectrometer. Ultraviolet–visible (UV–vis) absorption spectra were recorded on Shimadzu UV-2101 spectrophotometer in DMSO. Nuclear Magnetic Resonance (1H-NMR and 13C-NMR) spectra of compounds were recorded using a Bruker Advance III 300 MHz spectrometer in DMSO-d6 and TMS as an internal standard operating at 300 MHz for 1H-NMR and 75 MHz for 13 C-NMR. Thin layer chromatography (TLC) was carried out on Merck silica gel 60 F254 plates.
The ligands 3–(3-(4-fluorophenyl)triaz-1-en-1-yl) benzenesulfonamide (L1 ), 3–(3-(4-methoxyphenyl)triaz-1-en-1-yl) (L2 ), benzenesulfonamide 4–(3–(3-sulfamoylphenyl)triaz-2-en-1-yl) benzoic acide (L3 ), 3–(3-(3,4-dimethoxyphenyl)triaz-1-en-1-yl) benzenesulfonamide (L4 ), and 3-(3-(3,5-dimethylphenyl)triaz-1-en-1-yl)benzenesulfonamide (L5 ) were synthesised and characterised as previously described by us20 .
The ligands 3–(3-(4-fluorophenyl)triaz-1-en-1-yl) benzenesulfonamide (
7
Synthesis and Characterization of Novel Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Unless otherwise noted, all the chemicals and anhydrous solvents were purchased from Sigma-Aldrich, Merck, Alfa Aesar and TCI and used without further purification. Melting points (mp) were determined with SMP20 melting point apparatus and are uncorrected. FT-IR spectra were obtained by using Perkin Elmer Spectrum 100 FT-IR spectrometer. Nuclear Magnetic Resonance (1H-NMR and 13C-NMR) spectra of compounds were recorded using a Bruker Advance III 300 Mhz spectrometer in DMSO-d6 as the solvent, and TMS as the internal standard operating at 300 Mhz for 1H-NMR and 75 Mhz for 13C-NMR. Chemical shifts are expressed in ppm relative to tetramethylsilane. Splitting patterns are designated as singlet (s), doublet (d), triplet (t), quartette (q), and multiplet (m). Thin layer chromatography (TLC) was carried out on Merck silica gel 60 F254 plates.
8
Characterization of Photoreactive Biopolymers
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The inclusion of the acrylate-based photoreactive moieties in the different biopolymers was initially characterized by proton nuclear magnetic resonance ( 1 H NMR) spectroscopy. All spectra were acquired on a Bruker Advance III 300 MHz spectrometer. For NMR analysis all samples were dissolved in deuterated water (D2O) and transferred to 300 MHz NMR glass tubes (Wilmad, Cortenet, France). Samples were acquired with 256 scans, 8 dummy scans and with 18 secs of relaxation delay. The acquired spectra were processed by using the MestReNova v6.0.2 software. 1 H NMR data was used to determine HA degree of methacrylation as described in the literature [38, (link)39] (link).
In addition, the chemically modified ECM-mimetic biopolymers were characterized by attenuated total reflectance Fourier Transformed Infrared spectroscopy (ATR-FTIR). For analysis, powdered samples were placed in a Brucker Tensor 27 spectrometer. A total of 256 scans, with a spectral resolution of 4 cm -1 were acquired in the spectral window spanning from 4000 to 350 cm -1 . The obtained data was processed in OPUS software and plotted in Origin software (v9.1, trial version).
In addition, the chemically modified ECM-mimetic biopolymers were characterized by attenuated total reflectance Fourier Transformed Infrared spectroscopy (ATR-FTIR). For analysis, powdered samples were placed in a Brucker Tensor 27 spectrometer. A total of 256 scans, with a spectral resolution of 4 cm -1 were acquired in the spectral window spanning from 4000 to 350 cm -1 . The obtained data was processed in OPUS software and plotted in Origin software (v9.1, trial version).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!