Microbiological agar

Manufactured by Thermo Fisher Scientific

Sourced in United States

Microbiological agar is a solidifying agent used in the preparation of culture media for the growth and isolation of microorganisms. It is a polysaccharide derived from red seaweed that forms a gel-like solid when mixed with water and heated. Microbiological agar provides a stable, nutrient-rich matrix for the cultivation of bacteria, fungi, and other microbes in laboratory settings.

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Lab products found in correlation

Plant agar, by Duchefa Biochemie (1 mentions) Escherichia coli k12, by American Type Culture Collection (1 mentions)

2 protocols using microbiological agar

Agar-based Transformation Selection for Symbiodinium

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1.5% microbiological agar (Fisher Scientific, New Hampshire, US) or 0.8% plant agar (Duchefa Biochemie, Haarlem, The Netherlands) made up with filtered, autoclaved seawater enriched with f/2 medium was used to make agar plates for transformation selection. 100 μg/ml chloramphenicol or 200 to 1000 ng/ml atrazine was used as the selection antibiotic/herbicide depending on the construct being tested. Control plates with no selection antibiotic/herbicide were also made to confirm that the transformation method was mild enough that some cells survived the treatment. The samples were inoculated onto the agar plate using 3 ml of top agar made from 0.8% plant agar (Duchefa Biochemie, Haarlem, Netherlands) in f/2 medium with no antibiotics. This was done due to the tendency of Symbiodinium cells to clump, which prevented the cells from being easily evenly spread across agar surfaces. The plates were then grown in a LMS vertical incubator set to 26 °C with a 14:10 hours day:night cycle and 40 μmol photons m^-2 s^-1 light intensity for up to four months.

Chen J.E., Barbrook A.C., Cui G., Howe C.J, & Aranda M. (2019). The genetic intractability of Symbiodinium microadriaticum to standard algal transformation methods. PLoS ONE, 14(2), e0211936.

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Cultivation of S. aureus and E. coli

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Staphylococcus aureus 8325-4 and Escherichia coli K-12 (ATCC, Manassas, VA) were employed. Planktonic bacteria cells were cultured in brain heart infusion broth (BHI) (Fisher Scientific) with shaking at 37°C. PBS Response was used to wash microbial cells and for serial dilution. Liquid growth media was prepared from 200 ml of distilled water and 6 g of BHI powder. All liquid media were autoclaved at 120° C for 15 min before use. Solid growth media was prepared from liquid growth media with the addition of 1,5% microbiological agar (Fisher Scientific). A suspension of microbial cells was prepared by refreshing an overnight stationary phase culture in fresh medium for about 1 h. Cell pellets were isolated by centrifugation (13,690 × g for 5 min) and resuspended the appropriate volume of sterile PBS to give the desired cell-density (OD at 600 nm equivalent to 10⁸ CFU/ml).

Kasimova K.R., Sadasivam M., Landi G., Sarna T, & Hamblin M.R. (2014). Potentiation of photoinactivation of Gram-positive and Gram-negative bacteria mediated by six phenothiazinium dyes by addition of azide ion. Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology, 13(11), 1541-1548.

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