B6.129s7 ifngr1tm1agt j

All transgenic and WT mice presented here have a C57BL/6 background. C57BL/6J, Stat1^−/− [B6.129S(Cg)-Stat1^tm1Dlv/J], Ifngr1^−/− (B6.129S7-Ifngr1^tm1Agt/J), and CD45.1 (B6.SJL-Ptprc^a Pepc^b/ BoyJ) mice were purchased from Jackson Laboratories (Bar Harbor, Maine, USA) and housed under specific pathogen- and viral antibody-free conditions. All other strains used in this study were maintained under specific pathogen-free conditions. Cdcs1 Il10ra^−/− and Cdcs1 Il10ra^fl/flLyz2^Cre+ were generated as we have previously described⁷ . Cdcs1 (CD45.1) mice were generated by crossing Cdcs1 (B6.C3Bir-Cdcs1 (BC-R))^{46 (link)} and CD45.1 mice. Cdcs1 Il10ra^−/−Stat1^−/− mice were generated by crossing Cdcs1 Il10ra^−/− and Stat1^−/− [B6.129S(Cg)-Stat1^tm1Dlv/J] mice. Both male and female mice were used throughout the experiments, and wherever it was possible, we used littermate controls. At least 2 weeks before the initiation of an experiment bedding was mixed to standardize microbiota. The protocols for breeding, housing, and experiments were approved by the Animal Resources at Children’s Hospital, according to the Institutional Animal Care and Use Committees (Assurance number: A3303-01). Schematic diagrams describing experimental study designs were created with BioRender.com.

5- to 8-wk-old male and female, age-matched mice were used for all experiments. C57BL/6NCrl and B6.SJL-Ptprc^aPepc^b/BoyCrl were purchased from Charles River Laboratories. B6.129S2-Ifnar1^tm1Agt/Mmjax (Strain #:028288, Ifnar1^−/−), B6.129S(Cg)-Stat1^tm1Dlv/J (Strain #:012606, Stat1^−/−), B6.129S7-Ifngr1^tm1Agt/J (Strain #:003288, Ifngr1^−/−), B6.C(Cg)-Cd79a^tm1(cre)Reth/EhobJ (Strain #:020505, Mb1^Cre), B6.Cg-Tg(Cd4-cre)1Cwi/BfluJ (Strain #:022071, Cd4^Cre), B6.Cg-Foxp3tm2Tch/J (Strain #:006772, Foxp3^eGFP), and B6.129P2-Aicda^tm1(cre)Mnz/J (Strain #: 007770, Aicda^Cre/Cre) mice were purchased from The Jackson Laboratory. B6.Cg-Mx1^tm1.1Agsa/J (Mx1^gfp) were kindly provided by A. García-Sastre (Icahn School of Medicine at Mount Sinai) and have been described previously (32 (link)). Ifnlr1^tm1.1Svko (Ifnrl1^fl/fl) and Ifnlr1^tm1.2Svko (Ifnrl1^−/−) mice were kindly provided by Sergei V Kotenko (Rutgers New Jersey Medical School) and have been described previously (35 (link)). Tg(Tcrb51–11.5)AR1251Ayr (TClib^Tg) mice have been described previously (41 (link)). Mice were maintained and bred under specific pathogen-free conditions at the University of Minnesota. All animal experiments were approved by the Institutional Animal Care and Use Committee of the University of Minnesota. All animals were maintained under specific pathogen-free conditions at the University of Minnesota.

Female 7–12 week-old C57BL/6 mice (Jackson Laboratories, 000664), transgenic DsRed mice (Jackson Laboratories, 006051), and B6.129S7-Ifngr1^tm1Agt/J (referred to as IFNγR^−/− mice, Jackson Laboratories, 003288) were used for experiments. The investigators adhered to the ‘Guide for the Care and Use of Laboratory Animals’ as proposed by the committee on care of Laboratory Animal Resources Commission on Life Sciences, National Research Council. The facilities at the University of Florida Animal Care Services are fully accredited by the American Association for Accreditation of Laboratory Animal Care.